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High-nitrogen yeast and preparation method thereof

A yeast and high-nitrogen technology, applied in the field of Saccharomyces cerevisiae and its preparation, can solve the problems of high technical difficulty, complicated operation process, short mRNA storage time, etc., and achieve improved screening efficiency, high mutation rate, and easy radiation mutagenesis Effect

Active Publication Date: 2014-07-16
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantages are technical difficulty, short storage time of mRNA, and complicated operation process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: a kind of high nitrogen yeast, its main feature is Saccharomyces cerevisiae Saccharomyces cerevisiae, preserved by the General Microorganism Center of China Microbiological Culture Collection Management Committee, with the preservation number CGMCC No 5004;

[0038] The preparation method of high-nitrogen yeast is characterized in that it comprises the following steps:

[0039] (1) Prepare strains: Saccharomyces cerevisiae Saccharomyces cerevisiae: Saccharomyces cerevisiae 2399, which was purchased from the Institute of Microbiology, Chinese Academy of Sciences.

[0040] (2) Prepare solid medium by mass percentage glucose 3.0%-5.0%, peptone 0.5%-1.0%, yeast extract powder 0.2-1.0%, NH 4 SO 4 0.2%-3%, MgSO 4 0.03%-1.5%, KH 2 PO 4 0.2%-1.5%, agar 0.5%-1.5%, the rest is water;

[0041] (3) Preparation of liquid medium: by mass percentage, glucose 3.0%-5.0%, peptone 0.5%-1.0%, yeast extract powder 0.2-1.0%, NH 4 SO 4 0.2%-3%, MgSO 4 0.03%-1.5%, KH ...

Embodiment 2

[0064] Example 2: A high-nitrogen yeast, except for step (6) heavy ion irradiation treatment: when the bacterial suspension is observed to be turbid with the naked eye, it is diluted 1 to 100, and the bacterial suspension is subjected to heavy ion irradiation mutagenesis, and the irradiation dose is Be: 60Gy; All the other are the same as embodiment 1.

Embodiment 3

[0065] Example 3: A high-nitrogen yeast, except for step (6) heavy ion irradiation treatment: the bacterial suspension is observed to be turbid with the naked eye, and then diluted 1 to 102, and the bacterial suspension is subjected to heavy ion irradiation mutagenesis, and the irradiation dose is Be: 20Gy; All the other are the same as embodiment 1.

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Abstract

The invention relates to to a high-nitrogen yeast (particularly referring to Saccharomyces cerevisiae) and a preparation method thereof. Saccharomyces cerevisiae is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.5004; and Saccharomyces cerevisiae is obtained by heavy ion radiation mutation, the content of nitrogen in Saccharomyces cerevisiae is 40-60%. A heavy ion mutation technology has the following main advantages that (1) variation rate is high, generally 1000 times higher than natural variation rate; (2) variation spectrum is wide, namely type of variation is diverse, and a new type of microbe strain can be obtained; (3) variation stability is strong, relatively stable strains (coexistence of positive and negative mutations) can be obtained, and screening period can be shortened; and (4) mutation operability is strong, and the radiation mutation of the microbe can be easily completed.

Description

technical field [0001] The present invention relates to Saccharomyces cerevisiae and its preparation method. Background technique [0002] Baker's yeast belongs to Saccharomyces cerevisiae taxonomically. Yeast is a single-celled microorganism, its shape is round, oval, oval, and its size varies with the difference of strains. [0003] Yeast cells contain 65%-70% water, of which about 15% are free water; carbohydrates are 25%-35%, mostly in the form of polysaccharides; protein is 40%-60%, and yeast contains a complete amino acid group , including 8 kinds of amino acids necessary for the human body, especially lysine, which is less in grain protein, and higher in yeast. In addition, the amino acid ratio in yeast is close to the ideal amino acid composition value recommended by the Food and Agriculture Organization of the United Nations (FAO), so its nutritional value is high. The ash content is 5%-10%, and most of them contain more phosphorus and potassium. Yeast is also a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12N13/00C12N15/01C12R1/865
Inventor 马良肖国青王菊芳李文建
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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