Simple method for preparing golden mushroom liquid strain

A technology of liquid strains and Flammulina velutipes, applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problems of expensive cultivation equipment and complicated operation, shorten the time for germination, meet a large number of needs, and reduce production costs Effect

Inactive Publication Date: 2012-09-05
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by this invention is: aim at the above-mentioned deficiencies in the prior art, provide a kind of simple and easy method for preparing the liquid spawn of Flammulina velutipes, a kind of method that utilizes the conventional equipment of cultivating microorganisms to carry out the liquid spawn of Flammulina velutipes preparation, can overcome microbial liquid The defects of expensive deep culture equipment and complicated operation are convenient for mushroom farmers to carry out mass production of Flammulina velutipes liquid strains

Method used

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  • Simple method for preparing golden mushroom liquid strain
  • Simple method for preparing golden mushroom liquid strain
  • Simple method for preparing golden mushroom liquid strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Adopt aseptic operation method to inoculate the prepared mycelium suspension in the Erlenmeyer flask that sterilized liquid culture medium is housed, the culture medium filling capacity is 30% in the Erlenmeyer flask, and the suspension inoculum amount is 10%, Then place the triangular flask on a magnetic stirrer placed in a constant temperature incubator at 25°C, adjust the rotating speed to 180 rpm, and cultivate at a constant temperature of 25°C for 9 days, and the small yellow bacterial balls formed are liquid strains.

Embodiment 2

[0027] Adopt aseptic operation method to inoculate the prepared mycelium suspension in the triangular flask that the sterilized liquid culture medium is housed, the medium loading in the triangular flask is 40%, and the suspension inoculum size is 12%, Then place the triangular flask on a magnetic stirrer placed in a constant temperature incubator at 25°C, adjust the rotating speed to 190 rpm, and cultivate at a constant temperature of 25°C for 8 days, and the small yellow bacterial balls formed are liquid strains.

Embodiment 3

[0029] Adopt aseptic operation method to inoculate the prepared mycelium suspension in the Erlenmeyer flask that sterilized liquid culture medium is housed, the culture medium loading is 50% in the Erlenmeyer flask, and the suspension inoculation amount is 15%, Then place the triangular flask on a magnetic stirrer placed in a constant temperature incubator at 26°C, adjust the rotating speed to 200 rpm, and culture at a constant temperature of 26°C for 7 days, and the small yellow bacterial balls formed are liquid strains.

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Abstract

The invention relates to a simple method for preparing liquid needle mushroom spawns, which is characterized in that a sterile operation method is adopted to inoculate prepared mycelium suspension into a triangular flask in which sterilized liquid spawn culture medium is contained, and then the triangular flask is placed on a magnetic stirrer in a constant-temperature incubator for constant-temperature incubation to form yellow small fungus balls, i.e. the liquid spawns. By using conventional microorganism culture equipment for preparing the liquid needle mushroom spawns, the mass production of the liquid needle mushroom spawns can be conveniently conducted by mushroom farmers; and by using the liquid spawns to substitute for solid spawns to realize the culture of needle mushroom fruit bodies, the spawn running time can be shortened, the production cost can be reduced, the production efficiency can be improved and the great market demand can be satisfied.

Description

technical field [0001] The invention relates to the preparation of a liquid strain of Flammulina velutipes, in particular discloses a method for quickly cultivating liquid strains of Flammulina velutipes using simple equipment, which is suitable for mushroom farmers to mass-produce liquid strains of Flammulina velutipes. Background technique [0002] Flammulina velutipes not only contains protein, amino acids, vitamins, mineral elements and other nutrients, but also has nutritional functions, and is rich in polysaccharides, polypeptides, alkaloids, glycosides, allicin, enoki mushroom elements, shiitake mushrooms, etc. A variety of physiologically active substances have the functions of health care, disease prevention and anti-cancer, and can also promote the healthy growth and intellectual development of children. The use of Flammulina velutipes to produce health food and functional food has attracted worldwide attention. [0003] With the increasing demand for Flammulina v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 陈力力刘红靳磊陈冬纯
Owner HUNAN AGRICULTURAL UNIV
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