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Method for preparing high-purity ursolic acid and oleanolic acid from ligustrum lucidum ait

A technology for the joint preparation of oleanolic acid, which is applied in the field of natural medicines to achieve the effects of small processing volume, simple method and easy processing volume

Inactive Publication Date: 2012-02-15
曾超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported extraction method is mainly based on organic solution leaching in combination with certain separation means. For example, CN1358733 obtains ursolic acid in Ligustrum privet leaves by combining methods such as ethanol reflux extraction, activated carbon decolorization, drying, etc., although this invention is more convenient, But only crude ursolic acid can be obtained

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take 1Kg of dried privet leaves, add 8L of 5% sodium hydroxide aqueous solution, boil for 30 minutes, filter out the alkaline solution while hot, wash the medicinal residue with hot water, add the residue to 10L of water and boil for 1.5 hours, pour off the boiling liquid, The residue was refluxed and extracted with 95% ethanol for 3 times, and the amount of ethanol was 6L each time; the alcoholic extract was combined, and 0.3% activated carbon was added to the alcoholic extract, and stirred at 50-60°C for 1 hour for decolorization; The extract was adjusted to a pH of about 11 with 1mol / L sodium hydroxide aqueous solution, and filtered after standing for 12 hours to remove the precipitate, and the filtrate was concentrated under reduced pressure to about 1 / 3 (5L) of the volume before concentration; a large amount of insoluble matter was precipitated , this insoluble matter is the crude product of oleanolic acid, filtered, and the filtrate is adjusted to pH between 3-4 wi...

Embodiment 2

[0019] Take 1Kg of dried Ligustrum lucidum, add 8L of 5% sodium hydroxide aqueous solution, boil for 30 minutes, filter out the alkaline solution while hot, wash the medicinal residue with hot water, add the residue to 10L of water and boil for 2 hours, pour off the water and boil solution, the residue was refluxed with 90% ethanol for 3 times and the amount of ethanol was 6L each time; the alcohol extract was combined, and 0.3% of the volume of the alcohol extract was added with activated carbon, and stirred and decolorized at 50-60°C for 1 hour; the activated carbon was filtered off, The decolorized alcoholic extract is adjusted to a pH of about 11 with 1mol / L sodium hydroxide aqueous solution, filtered after standing for 12 hours, and the precipitate is removed, and the filtrate is concentrated under reduced pressure to about 1 / 3 (5L) of the volume before concentration; there is a large amount of insoluble The insoluble matter is the crude product of oleanolic acid, which is...

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Abstract

The invention discloses a method for preparing high-purity ursolic acid and oleanolic acid from ligustrum lucidum ait. The ligustrum lucidum ait is ligustrum lucidum ait leaf or ligustrum lucidum ait fruit. The ligustrum lucidum ait leaf or the ligustrum lucidum ait fruit is used as a raw material in the preparation method. The preparation method comprises the following steps of: removing impurities from the ligustrum lucidum ait leaf or the ligustrum lucidum ait fruit by using alkali water, boiling, extracting with ethanol, decolorizing with active carbon, regulating alkali, removing impurities, performing reduced pressure concentration to separate out a crude oleanolic acid product, regulating acid to separate out a crude ursolic acid product, and purifying the oleanolic acid and the ursolic acid by washing, re-crystallization and the like. The cheap and easily obtained ligustrum lucidum ait leaf or ligustrum lucidum ait fruit is used as a raw material, and the ethanol is used for reflux and extraction. The treatment amount of 'three wastes' is low, the 'three wastes' are easily treated, the ethanol can be recycled, and the waste residue left after extraction can be used as a fertilizer. Therefore, the method basically does not produce environmental pollution, the yield is high, and the products are pure. In addition, the method is simple, convenient, fast and suitable for factory large-scale production.

Description

technical field [0001] The invention belongs to the technical field of natural medicines. Specifically, it relates to extracting and purifying the main active ingredients ursolic acid and oleanolic acid from privet privet. technical background [0002] Ligustrum is a plant of the genus Ligustrum of the Oleaceae family. The scientific name is Ligustrum Lucidum Ait. It is a large evergreen shrub or small tree in all seasons. There are about 50 species in the world, and there are 38 species in my country. They are widely distributed in East China, South China, Southwest China and Central China is rich in plant resources and widely distributed. [0003] With the deepening of pharmacological research, it is found that privet plants have various physiological activities, but the research on the chemical constituents of privet plants is not enough. For a long time, the parts of the privet plant we have studied have not been fully utilized, basically not used as medicine, and there...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 司福亭李靖靖姚虹周晓莉司方博付鸿宁建中岳福兴高建炳王文武王宇飞华慧颖曾超
Owner 曾超
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