Method for preparing high-purity ursolic acid and oleanolic acid from ligustrum lucidum ait

A technology for the joint preparation of oleanolic acid, which is applied in the field of natural medicines to achieve the effects of small processing volume, simple method and easy processing volume

Inactive Publication Date: 2012-02-15
曾超
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported extraction method is mainly based on organic solution leaching in combination with certain separation means. For example, CN1358733 obtains ursolic acid in Ligustrum privet leaves

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0012] Example 1:

[0013] Take 1Kg of Ligustrum lucidum dry product, add 8L of 5% sodium hydroxide aqueous solution, boil for 30 minutes, filter out the lye solution while hot, wash the medicine residue with hot water, add the residue to 10L of water and boil for 1.5 hours, pour out the boiled solution, The residue was refluxed and extracted with 95% ethanol for 3 times, the amount of ethanol is 6L each time; combine the alcohol extracts, add 0.3% of the volume of the alcohol extracts with activated carbon, stir and decolorize at 50-60℃ for 1 hour; filter out the activated carbon and decolorize the alcohol Adjust the pH of the extract to about 11 with 1mol / L sodium hydroxide aqueous solution. After standing for 12 hours, filter to remove precipitation. The filtrate is concentrated under reduced pressure to about 1 / 3 of the volume before concentration (5L); a large amount of insoluble matter precipitates The insoluble matter is crude oleanolic acid, filtered, and the filtrate is ...

Example Embodiment

[0018] Example 2:

[0019] Take 1Kg of Ligustrum lucidum dry product, add 8L of 5% sodium hydroxide aqueous solution, boil for 30 minutes, filter out the lye liquid while hot, wash the medicine residue with hot water, add the residue to 10L of water and boil for 2 hours, pour out the water and boil The liquid and the residue were refluxed and extracted with 90% ethanol for 3 times, each time the amount of ethanol was 6L; combined the alcohol extracts, added 0.3% of the volume of the alcohol extracts with activated carbon, stirred and decolorized at 50-60°C for 1 hour; filtered off the activated carbon, Adjust the pH of the decolorized alcohol extract to about 11 with 1mol / L sodium hydroxide aqueous solution, let it stand for 12 hours and then filter to remove precipitation. The filtrate is concentrated under reduced pressure to about 1 / 3 of the volume before concentration (5L); there is a large amount of insoluble The insoluble matter is the crude oleanolic acid, filtered, and th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing high-purity ursolic acid and oleanolic acid from ligustrum lucidum ait. The ligustrum lucidum ait is ligustrum lucidum ait leaf or ligustrum lucidum ait fruit. The ligustrum lucidum ait leaf or the ligustrum lucidum ait fruit is used as a raw material in the preparation method. The preparation method comprises the following steps of: removing impurities from the ligustrum lucidum ait leaf or the ligustrum lucidum ait fruit by using alkali water, boiling, extracting with ethanol, decolorizing with active carbon, regulating alkali, removing impurities, performing reduced pressure concentration to separate out a crude oleanolic acid product, regulating acid to separate out a crude ursolic acid product, and purifying the oleanolic acid and the ursolic acid by washing, re-crystallization and the like. The cheap and easily obtained ligustrum lucidum ait leaf or ligustrum lucidum ait fruit is used as a raw material, and the ethanol is used for reflux and extraction. The treatment amount of 'three wastes' is low, the 'three wastes' are easily treated, the ethanol can be recycled, and the waste residue left after extraction can be used as a fertilizer. Therefore, the method basically does not produce environmental pollution, the yield is high, and the products are pure. In addition, the method is simple, convenient, fast and suitable for factory large-scale production.

Description

technical field [0001] The invention belongs to the technical field of natural medicines. Specifically, it relates to extracting and purifying the main active ingredients ursolic acid and oleanolic acid from privet privet. technical background [0002] Ligustrum is a plant of the genus Ligustrum of the Oleaceae family. The scientific name is Ligustrum Lucidum Ait. It is a large evergreen shrub or small tree in all seasons. There are about 50 species in the world, and there are 38 species in my country. They are widely distributed in East China, South China, Southwest China and Central China is rich in plant resources and widely distributed. [0003] With the deepening of pharmacological research, it is found that privet plants have various physiological activities, but the research on the chemical constituents of privet plants is not enough. For a long time, the parts of the privet plant we have studied have not been fully utilized, basically not used as medicine, and there...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07J63/00
Inventor 司福亭李靖靖姚虹周晓莉司方博付鸿宁建中岳福兴高建炳王文武王宇飞华慧颖曾超
Owner 曾超
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap