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Preparation method of preeclampsia detection kit adopting Adipsin as detection index

A detection kit and preeclampsia technology, which is applied to measuring devices, instruments, and disease diagnosis, can solve the problems of undiscovered, cumbersome and time-consuming sample collection methods, and achieve the effect of improving accuracy

Active Publication Date: 2014-03-19
ORIGISSAY BIOLOGICS TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of collecting samples by this method is cumbersome and time-consuming
At present, there is no report on using the Adipsin content in the urine sample as the detection index of preeclampsia, and there is no report on the preeclampsia detection kit and its preparation method using the Adipsin content in the urine sample as the detection index.

Method used

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  • Preparation method of preeclampsia detection kit adopting Adipsin as detection index
  • Preparation method of preeclampsia detection kit adopting Adipsin as detection index
  • Preparation method of preeclampsia detection kit adopting Adipsin as detection index

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] In this embodiment, the following process steps are used to prepare the preeclampsia detection kit with Adipsin as the detection index:

[0046] (1) Preparation of multi-well plates coated with mouse anti-human Adipsin monoclonal antibody

[0047] Dilute the mouse anti-human Adipsin monoclonal antibody to a diluent with a concentration of 10 μg / mL with carbonate buffer solution of pH=9, add anhydrous methanol to the antibody diluent, and the addition amount of anhydrous methanol should reach a concentration of 3 mL / mL 100mL as the limit, then add the methanol-containing mouse anti-human Adipsin monoclonal antibody dilution into each well of a 96-well plate in an amount of 100 μL / well, and coat at 4°C for 12 hours; after the coating time expires, Bovine serum albumin solution with a concentration of 1g / 100mL was added to each well of the multi-well plate in an amount of 120 μL / well, and the blocking reaction was carried out at 37°C. The reaction time was 1 hour; mol / L p...

Embodiment 2

[0053] In this example, the preeclampsia detection kit prepared in Example 1 is used to detect the samples to be tested.

[0054] 1. Sample to be tested

[0055] (1) Standard sample preparation

[0056] The Adipsin protein standard that is equipped in the kit described in Example 1 is set as the standard sample containing the highest concentration of Adipsin, named as standard sample 1, and the concentration of Adipsin is 6ng / mL, and then used in the kit described in Example 1 The prepared sample diluent dilutes the Adipsin protein standard into standard samples with Adipsin concentrations of 2000pg / mL, 666.7pg / mL, 222.2pg / mL, 74.07pg / mL, 24.69pg / mL, and 8.23pg / mL, They are named Standard Sample 2, Standard Sample 3, Standard Sample 4, Standard Sample 5, Standard Sample 6, and Standard Sample 7 in sequence, and there are 7 standard samples in total.

[0057] (2) Sample

[0058] Pregnant women who were outpatient and hospitalized in West China Second Hospital of Sichuan Univ...

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Abstract

A preeclampsia detecting kit using Adipsin as indicator, comprises porous plate coated with anti-human Adipsin monoclonal antibody, anti-human Adipsin monoclonal antibody detecting solution marked by biotin, avidin horseradish peroxidase combined with anti-human Adipsin monoclonal antibody marked by biotin, chromogenic substrate 3',3',5,5'-tetramethylbenzidine and Adipsin protein standard substance. The preparing method of the detecting kit comprises:(1)preparing the porous plate coated with anti-human Adipsin monoclonal antibody; (2) preparing the anti-human Adipsin monoclonal antibody detecting solution marked by biotin;(3) preparing the avidin horseradish peroxidase combined with anti-human Adipsin monoclonal antibody marked by biotin;(4) preparing the chromogenic substrate 3',3',5,5'-tetramethylbenzidine and Adipsin protein standard substance.

Description

technical field [0001] The invention belongs to the field of medical kits, in particular to a kit for detecting preeclampsia (PE for short) in pregnant women and a preparation method thereof. Background technique [0002] Preeclampsia (PE for short) is an obstetric complication that occurs after 20 weeks of pregnancy and is one of the leading causes of maternal and perinatal death. The incidence rate of preeclampsia among pregnant women worldwide is 2-8%, and the incidence rate among pregnant women in my country can reach 10%. Patients with severe preeclampsia may have serious clinical manifestations such as hypertension, edema, and severe proteinuria, and are also prone to adverse outcomes such as pulmonary edema, renal failure, and HELLP syndrome, and suffer from long-term cardiovascular disease, kidney disease, and metabolic disease. The risk of disease is also significantly increased. For the fetus, the smaller the gestational age, the lower the survival rate. The mort...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
CPCG01N33/689G01N2800/368
Inventor 胡怀忠
Owner ORIGISSAY BIOLOGICS TECH
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