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Elastase inhibitor

An elastase and inhibitor technology, applied in the field of elastase inhibitors, can solve problems such as reduced skin elasticity

Active Publication Date: 2012-05-02
SHISEIDO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, elastin contributes to the elasticity of tissues by forming cross-links with each other, but it is considered that elastin is modified and destroyed due to overexpression of elastase, which is an elastin-destroying enzyme, with ultraviolet exposure and / or aging. Decreased skin elasticity

Method used

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Examples

Experimental program
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Embodiment

[0041] Hereinafter, although an Example is given and this invention is demonstrated concretely, this invention is not limited to the following Example.

[0042] As a reaction buffer, 0.1M HEPES, 0.5M NaCl (pH 7.5) were used. As an elastase substrate, oxysuccinyl-(Ala) 2 - Pro-Val-MCA (Cat No. 3153-v of Peptide Research Institute) was dissolved in DMSO to 80 mM, and diluted to 0.16 mM with a reaction buffer. Human leukocyte-derived elastase (ELASTIN PRODUCT CO., INC. Cat No. CK828) was diluted to 5 µg / mL with a reaction buffer.

[0043] 25 μL of 0.16 mM-elastase substrate was dispensed into each 96-well plate, and 50 μL of each drug was added. Then, 25 μL of 5 μg / ml elastase was added on ice, incubated at 37° C. for 60 minutes, and then the fluorescence at 460 nm was measured with an excitation wavelength of 369 nm. As raspberry extract, Raspberry Extract BG from Maruzen Pharmaceutical Co., Ltd. was used, as hydroxyproline, L-hydroxyproline from Kyowa Hakko Kogyo Co., Ltd. w...

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PUM

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Abstract

An elastase inhibitor contains raspberry extract and hydroxyproline as active ingredients, and optionally contains yeast extract.

Description

technical field [0001] The present invention relates to an elastase inhibitor comprising raspberry extract and hydroxyproline, which are plant-derived components, as active ingredients. Background technique [0002] In the past, the necessity of anti-aging agents has been considered, but since the mechanism and / or definition of aging have not been clarified, it is generally used to measure the moisture level of the skin and / or the elasticity of the skin It can be judged by measuring or visually observing the skin color. However, in recent years, aging-related research has progressed. As the cause of skin aging, aging is an important cause from a macroscopic point of view. In addition, dryness, oxidation, and the influence of sunlight (ultraviolet rays) are also directly related to skin aging. Reasons are listed. As specific phenomena of skin aging, reduction of collagen and / or elastin in the dermis of the skin, reduction of mucopolysaccharides including hyaluronic acid, da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61K8/44A61K8/97A61K31/401A61K36/06A61P17/00A61P29/00A61P43/00A61Q19/00A61Q19/08
CPCA61K8/97A61K8/4913A61K31/401A61K8/99A61Q19/08A61K36/06A61K36/73A61K8/9789A61K8/9728A61P11/00A61P17/00A61P19/02A61P29/00A61P37/00A61P43/00A61P9/00A61K2300/00A61K2800/782
Inventor 饭野雅人
Owner SHISEIDO CO LTD
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