Method and kit for determining apolipoprotein A2 by using immunity transmission turbidimetric method

An immune transmission turbidimetry and apolipoprotein technology, which is applied in biological testing, material analysis by observing the influence of chemical indicators, and material inspection products, etc. It can solve complex sample pretreatment, high equipment requirements, and anti-interference ability. Poor problems, to achieve the effect of simple operation and pretreatment process, strong anti-interference ability

Inactive Publication Date: 2012-06-20
BEIJING LEADMAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] An object of the present invention is to overcome the cumbersome operation of the existing method for measuring apolipoprotein A2, high equipment requirements, complicated sample pretreatment, and batch detection and analysis cannot be directly carried out on a full-automatic biochemical analyzer. The shortcomings of the sample's po

Method used

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  • Method and kit for determining apolipoprotein A2 by using immunity transmission turbidimetric method
  • Method and kit for determining apolipoprotein A2 by using immunity transmission turbidimetric method
  • Method and kit for determining apolipoprotein A2 by using immunity transmission turbidimetric method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 1. ApoA II reactant (R1)

[0060]

[0061] 2. Anti-ApoA II antibody solution (R2)

[0062]

[0063] 3. Liquid serotype ApoA II constant value calibrator

[0064]

[0065] The rest is purified water

[0066] Add the corresponding amount of Apoa II antigen to the above solution according to the required concentration of the constant value calibration solution to prepare the Apoa II constant value calibration solution. In this example, a high-concentration single-point reference calibrator was selected, and the Apoa II antigen was added at a concentration of 0.7g / L, and then sterilized by suction with a 0.22μm filter membrane, and stored at 2-8°C. When used, dilute with normal saline to obtain reference calibration products with different concentration gradients, in order: the first point is 0g / L; the second point is 0.18g / L; the third point is 0.35g / L; the fourth point is 0.47 g / L; the fifth point is 0.7g / L.

[0067] Reagents R1 and R2 are mixed in a volume r...

Embodiment 2

[0069] 1. ApoA II reactant (R1)

[0070]

[0071] 2. Anti-ApoA II antibody solution (R2)

[0072]

[0073] 3. Liquid serotype ApoA II constant value calibrator

[0074]

[0075]

[0076] The liquid serotype constant value calibrator is divided into five parts, according to different concentration standards (the first point is 0g / L; the second point is 0.18g / L; the third point is 0.35g / L; the fourth point is 0.47g / L; the fifth point is 0.7g / L) Add the ApoA II antigen to the above five solutions, then use a 0.22μm filter membrane to filter and sterilize, and store at 2-8°C.

[0077] Reagents R1 and R2 were mixed in a volume ratio of 5:1 to be used as a single reagent.

Embodiment 3

[0079] 1. ApoA II reactant (R1)

[0080]

[0081] 2. Anti-ApoA II antibody solution (R2)

[0082]

[0083] 3. Liquid serotype ApoA II constant value calibrator

[0084]

[0085]

[0086] In this example, a high-concentration single-point reference calibrator was selected, and the Apoa II antigen was added at a concentration of 0.7g / L, and then sterilized by suction with a 0.22μm filter membrane, and stored at 2-8°C. When used, dilute with normal saline to obtain reference calibration products with different concentration gradients, in order: the first point is 0g / L; the second point is 0.18g / L; the third point is 0.35g / L; the fourth point is 0.47 g / L; the fifth point is 0.7g / L.

[0087] Reagents R1 and R2 are mixed in a volume ratio of 3:1 to be used as a single reagent. Set parameters in the automatic biochemical analyzer (Olympus AU400) as follows: reaction temperature 37°C, main wavelength: 410nm, secondary wavelength: 660nm, two-point rate method (RATE1), R1...

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Abstract

The invention provides a method for determining apolipoprotein A2 by using an immunity transmission turbidimetric method, and a kit composed of applied reagents. The reagents applied by the method comprises one or more stabilizing agent selected from substances of: glycol(alpha-amino ethyl)ether tetraacetic acid, iminodiacetic acid, sodium polyoxyethylene fatty alcohol ether sulfate (AES), 1,2-cylohexanediol diglycidol, sodium diethylene triamine pentacetate, and hexapolyglycerol dioleates; and one or more high-molecular accelerating agents selected from substances of: fatty alcohol polyoxyethylene ether, polyvinylpyrrolidone, glycol polyoxylethylene ether, hydroxypropyl methylcellulose, carboxymethyl cellulose, and sodium polyacrylate. The method provided by the invention can be used in batch detection and analysis. The method and the kit have high anti-interference capabilities against high fat, jaundice and hemolysis samples.

Description

technical field [0001] The present invention relates to a method for measuring apolipoprotein A2 and a kit used in the method, in particular the invention relates to a method for measuring apolipoprotein A2 by immunoturbidimetry and a kit used in the method. Background technique [0002] Apolipoprotein A II (ApoA II) is the second most abundant apolipoprotein among high-density lipoproteins (HDL). ApoA II is composed of 77 amino acid residues of two polypeptide chains. Its molecular weight is 17000D measured in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) without reducing agent. In human plasma Exists in dimer form. The monomeric molecular weight of ApoA II is 8700D. The C-terminal amino acid residue of human ApoA II protein is glutamic acid, the N-terminal is pyrrolidonic acid, and lacks histidine, arginine and tryptophan. ApoA II has polymorphisms, the main polymorphic isoelectric point is 4.9, and the isoelectric point of the less polymorphic is...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/82
Inventor 不公告发明人
Owner BEIJING LEADMAN BIOCHEM
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