Aluminum hydroxide gel-polysaccharide composite immunologic adjuvant and preparation method and application thereof

A compound immune adjuvant and aluminum hydroxide gel technology, which is applied in the field of biomedicine, can solve the problems of incomplete understanding of the mechanism of action of aluminum salt adjuvants, and achieve the effects of strong immune activity, high immune effect, and high clinical safety

Active Publication Date: 2012-07-04
WEST VAC BIOPHARMA CO LTD
View PDF7 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aluminum hydroxide or aluminum phosphate plays an important role in promoting the absorptio...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aluminum hydroxide gel-polysaccharide composite immunologic adjuvant and preparation method and application thereof
  • Aluminum hydroxide gel-polysaccharide composite immunologic adjuvant and preparation method and application thereof
  • Aluminum hydroxide gel-polysaccharide composite immunologic adjuvant and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Screening and preparation of compound adjuvants

[0043]The present invention compares liposomes, aluminum hydroxide gels, hydrogels, mannans, polysaccharides (derived from Escherichia coli or Bacillus typhi), etc. individually or in combination as adjuvants to add antigens, and finds that aluminum hydroxide gels / Bacterial polysaccharide (Al-PS) combination as a compound adjuvant can effectively induce both humoral immunity and cellular immunity.

[0044] This compound adjuvant uses two components: aluminum hydroxide gel adjuvant, which is a kind of Al-containing adjuvant. 3+ The inorganic salt has good adsorption effect and can adsorb soluble antigens on the surface of aluminum glue molecules; it is also a good precipitant, which can concentrate antigens and reduce the injection dose. Aluminum hydroxide gel is the most widely used adjuvant and the only adjuvant approved by the FDA for human vaccines so far. Polysaccharides, especially bacterial-derived pol...

Embodiment 2

[0060] Example 2 The present invention uses E. coli polysaccharide or typhoid polysaccharide as an animal immunization test for hepatitis B vaccine adjuvant

[0061] 1. Grouping

[0062] 1. Individual protein group: HBsAg protein 0.1 μg / mouse / time;

[0063] 2. Al(OH) 3 Group: HBsAg protein 0.1μg+Al(OH) 3 25μg / mouse / time;

[0064] 3. E.coli polysaccharide group: HBsAg protein 0.1 μg+E.coli polysaccharide 20 μg / mouse / time;

[0065] 4. Al(OH) 3 +E.coli polysaccharide group: HBsAg protein 0.1μg+Al(OH) 3 25μg+E.coli polysaccharide 20μg / mouse / time;

[0066] 5. PBS group: PBS+Al(OH) 3 25μg+E.coli polysaccharide 20μg / mouse / time.

[0067] 6-week-old C57BL / 6 mice (five mice in each group) were immunized according to the above groups, once every two weeks, three times in total. Blood was collected from the tail vein at 0, 1, 3, 5, 7, 9, 13, and 17 weeks after immunization, respectively, and the serum was separated and stored at -20°C for testing.

[0068] 2. Determination of ...

Embodiment 3

[0083] Embodiment 3 The present invention uses E. coli polysaccharide or typhi polysaccharide as bFGF adjuvant animal immunization test

[0084] 1. Grouping

[0085] 1.bFGF+Al(OH) 3 Group: bFGF protein 20μg+Al(OH) 3 500μg / mouse / time;

[0086] 2. bFGF+E.coli polysaccharide group: bFGF protein 20μg+E.coli polysaccharide 20μg / mouse / time;

[0087] 3. bFGF+Al(OH) 3 +E.coli polysaccharide group: bFGF protein 20μg+Al(OH) 3 500μg+E.coli polysaccharide 20μg / mouse / time;

[0088] 4. bFGF + Freund's adjuvant group: bFGF protein 20μg + Freund's adjuvant / mouse / time;

[0089] 5. PBS group: PBS+Al(OH) 3 500μg+E.coli polysaccharide 20μg / mouse / time.

[0090] 6-week-old C57BL / 6 mice (seven mice in each group) were immunized according to the above groups, once every two weeks, three times in total. Blood was collected from the tail vein at 0, 1, 2, 3, 4, 5, and 6 weeks after immunization, and the serum was separated and stored at -20°C for testing. Humoral immunity and cellular immuni...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the field of biomedicine, and particularly relates to an aluminum hydroxide gel-polysaccharide composite immunologic adjuvant and a preparation method and application thereof. The invention aims to provide a new immunologic adjuvant with good performance. The technical scheme is to provide a composite immunologic adjuvant. The composite immunologic adjuvant mainly contains aluminum hydroxide gel and polysaccharide serving as bacteria source. The aluminum hydroxide gel-polysaccharide composite immunologic adjuvant provided by the invention has the advantages of strong immunocompetence, high clinical safety and the like, and is an excellent composite immunologic adjuvant aiming at various antigens. The hepatitis vaccine and tumor vaccine prepared by taking the aluminum hydroxide gel-polysaccharide composite provided by the invention as adjuvant has stronger immune effect and anti-cancer effect, and thus new selection is provided for the development and application of vaccine.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an aluminum hydroxide-polysaccharide composite immune adjuvant and a preparation method and application thereof. Background technique [0002] Adjuvants have been used for decades to improve the body's immune response to vaccine antigens. The purpose of adding adjuvant to the vaccine is to enhance the immunogenicity and immune protection effect of the antigen, which is manifested as enhancing the specific humoral immunity and / or cellular immune response of the specific antigen, thereby improving the production or / and specificity of the specific antibody. Cellular immune function, reducing the amount of antigen required for effective immunization, reducing the frequency of need for booster immunization, improving early immune response and the response success rate of immunocompromised patients, etc. [0003] Aluminum adjuvants represented by aluminum hydroxide and aluminum ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/39A61K39/00A61K39/29A61P1/16A61P31/20A61P35/00
CPCY02A50/30
Inventor 杨莉魏于全王永生石华山
Owner WEST VAC BIOPHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap