Aluminum hydroxide gel-polysaccharide composite immunologic adjuvant and preparation method and application thereof

A technology of aluminum hydroxide gel and compound immune adjuvant, applied in the field of biomedicine, can solve the problem that the action mechanism of aluminum salt adjuvant is not completely clear, and achieve the effects of strong immune activity, high anti-tumor effect, and high immune effect.

Active Publication Date: 2015-07-22
WEST VAC BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aluminum hydroxide or aluminum phosphate plays an important role in promoting the absorption of antigens, but the mechanism of action of aluminum salt adjuvants is not yet fully understood

Method used

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  • Aluminum hydroxide gel-polysaccharide composite immunologic adjuvant and preparation method and application thereof
  • Aluminum hydroxide gel-polysaccharide composite immunologic adjuvant and preparation method and application thereof
  • Aluminum hydroxide gel-polysaccharide composite immunologic adjuvant and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Screening and preparation of composite adjuvants

[0043]The present invention compares liposome, aluminum hydroxide gel, hydrogel, mannan, polysaccharide (E. / bacterial polysaccharide (Al-PS) combination as a compound adjuvant can not only effectively induce the body to produce humoral immunity, but also effectively induce cellular immunity.

[0044] This composite adjuvant uses two components: among them, aluminum hydroxide gel adjuvant is a kind of adjuvant containing Al 3+ The inorganic salt has good adsorption effect, and can adsorb soluble antigens on the molecular surface of aluminum gel; it is also a good precipitant, which can concentrate antigens and reduce the injection dose. Aluminum hydroxide gel is cheap, easy to use, and non-toxic. It is the most widely used adjuvant, and it is also the only adjuvant approved by the FDA for human vaccines. Polysaccharides, especially polysaccharides derived from bacteria, as a natural source of adjuvants, have...

Embodiment 2

[0060] Embodiment 2 The present invention uses E.coli polysaccharide or typhoid polysaccharide as animal immunity test of hepatitis B vaccine adjuvant

[0061] 1. Grouping

[0062] 1. Individual protein group: HBsAg protein 0.1 μg / mouse / time;

[0063] 2.Al(OH) 3 Group: HBsAg protein 0.1μg+Al(OH) 3 25μg / mouse / time;

[0064] 3. E.coli polysaccharide group: HBsAg protein 0.1 μg + E.coli polysaccharide 20 μg / mouse / time;

[0065] 4.Al(OH) 3 +E.coli polysaccharide group: HBsAg protein 0.1μg+Al(OH) 3 25μg+E.coli polysaccharide 20μg / mouse / time;

[0066] 5. PBS group: PBS+Al(OH) 3 25μg+E.coli polysaccharide 20μg / mouse / time.

[0067] The 6-week-old C57BL / 6 mice (five mice in each group) were immunized according to the above grouping, once every two weeks, and three times in total. Blood was collected from the tail vein at 0, 1, 3, 5, 7, 9, 13, and 17 weeks after immunization, and the serum was separated and stored at -20°C for testing.

[0068] Second, the determination of p...

Embodiment 3

[0083] Embodiment 3 The present invention uses E.coli polysaccharide or Salmonella typhi polysaccharide as bFGF adjuvant animal immunity test

[0084] 1. Grouping

[0085] 1.bFGF+Al(OH) 3 Group: bFGF protein 20μg+Al(OH) 3 500μg / mouse / time;

[0086] 2. bFGF+E.coli polysaccharide group: bFGF protein 20μg+E.coli polysaccharide 20μg / mouse / time;

[0087] 3.bFGF+Al(OH) 3 +E.coli polysaccharide group: bFGF protein 20μg+Al(OH) 3 500μg+E.coli polysaccharide 20μg / mouse / time;

[0088] 4. bFGF+Freund's adjuvant group: bFGF protein 20μg+Freund's adjuvant / mouse / time;

[0089] 5. PBS group: PBS+Al(OH) 3 500μg+E.coli polysaccharide 20μg / mouse / time.

[0090] The 6-week-old C57BL / 6 mice (seven in each group) were immunized according to the above grouping, once every two weeks, and three times in total. Blood was collected from the tail vein at 0, 1, 2, 3, 4, 5, and 6 weeks after immunization, the serum was separated, and stored at -20°C for testing, and the humoral immunity and cell...

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Abstract

The invention belongs to the field of biomedicine, and particularly relates to an aluminum hydroxide gel-polysaccharide composite immunologic adjuvant and a preparation method and application thereof. The invention aims to provide a new immunologic adjuvant with good performance. The technical scheme is to provide a composite immunologic adjuvant. The composite immunologic adjuvant mainly contains aluminum hydroxide gel and polysaccharide serving as bacteria source. The aluminum hydroxide gel-polysaccharide composite immunologic adjuvant provided by the invention has the advantages of strong immunocompetence, high clinical safety and the like, and is an excellent composite immunologic adjuvant aiming at various antigens. The hepatitis vaccine and tumor vaccine prepared by taking the aluminum hydroxide gel-polysaccharide composite provided by the invention as adjuvant has stronger immune effect and anti-cancer effect, and thus new selection is provided for the development and application of vaccine.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an aluminum hydroxide-polysaccharide compound immune adjuvant and its preparation method and application. Background technique [0002] Adjuvants have been used for decades to improve the body's immune response to vaccine antigens. The purpose of adding an adjuvant to the vaccine is to enhance the immunogenicity and immune protection effect of the antigen, which is manifested by enhancing the specific humoral immunity and / or cellular immune response of the specific antigen, thereby improving the production or / and specificity of specific antibodies Cellular immune function, reducing the amount of antigen required for effective immunization, reducing the frequency of booster immunizations, improving the success rate of early immune responses and responses in immunocompromised persons, etc. [0003] Aluminum adjuvants represented by aluminum hydroxide and aluminum phosphate a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/00A61K39/29A61P1/16A61P31/20A61P35/00
CPCY02A50/30
Inventor 杨莉魏于全王永生石华山
Owner WEST VAC BIOPHARMA CO LTD
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