Method for inducing bloom of dendrobium candidum in test tube

A Dendrobium officinale, test tube flowering technology, applied in the field of agricultural plant biology, can solve the problems of small flowers, incomplete flower organs, weak plant growth, etc., achieve the effect of large flower shape and increase flower formation rate

Inactive Publication Date: 2013-07-17
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the research reports on the flowering of Dendrobium officinale in test tubes, hormones of different concentrations such as 6-BA and NAA are often used to induce them, or a certain amount of PP333 , TDZ, etc. are added to induce them, although a certain amount can be obtained. Flowering induction rate [1, 14, 15], but the plant growth is weak, the leaves are curled, the flowers are small, and flowers with incomplete floral organs often appear

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Firstly, the protocorm (MS+1.0 mg·L -1 6-BA+1.0mg·L -1 NAA+30 g·L -1 Sucrose+7.0 g·L -1 agar, medium with pH 5.4 for the induction and proliferation of protocorms), and then the protocorms were cultured and induced as follows:

[0016] (1) Strong seedling culture stage: with 1 / 2 MS as the basic medium, 3% sucrose (w / v), adding NAA0.5 mg / L and 6-BA 2.0 mg / L under the culture conditions, under the light intensity of 40 mu mol.m -2 .s -1 , the wavelength is 600 nm, the light time is 12 h / d, and the culture temperature is 25°C, the strong seedlings are cultivated for 60 days.

[0017] (2) Induce flowering stage I: use 1 / 2 MS as basic medium, add PP 333 1.00 mg / L+ABA 0.5 mg / L, under light intensity of 60 mu mol.m -2 .s -1 , wavelength 600nm, culture temperature 26 ℃, additional banana juice 14% (v / v), activated carbon 0.15% culture conditions, after 23 days of culture, it enters the induction stage II.

[0018] (3) Induction of flowering stage II: 1 / 2 MS as the...

Embodiment 2

[0022] Adopt the protocorm of dendrobium officinale stem section cultured in vitro to carry out following cultivation and induction:

[0023] (1) Strong seedling cultivation stage: with 1 / 2 MS as the basic medium, 3% sucrose (w / v), adding NAA 0.8 mg / L and 6-BA 1.7 mg / L in the culture conditions, under the light intensity of 35 mu mol.m -2 .s -1 , the wavelength is 650 nm, the light time is 14 h / d, and the culture temperature is 24°C, and the strong seedlings are cultivated for 55 days.

[0024] (2) Induce flowering stage I: use 1 / 2 MS as basic medium, add PP 333 0.80 mg / L+ABA 0.8 mg / L, at a light intensity of 55 mu mol.m -2 .s -1 , wavelength 660 nm, culture temperature 25 ℃, additional banana juice 10% (v / v), activated carbon 0.3% culture conditions, after 25 days of culture, it entered the induction stage II.

[0025] (3) Induction of flowering stage II: 1 / 2 MS as the basic medium, adding TDZ 0.05 mg / L + BA 1.0 mg / L, at a light intensity of 55 mu mol.m -2 .s -1 ,...

Embodiment 3

[0029] Adopt the protocorm of dendrobium officinale stem section cultured in vitro to carry out following cultivation and induction:

[0030] (1) Strong seedling cultivation stage: with 1 / 2 MS as the basic medium, 3% sucrose (w / v), adding NAA1. mu mol.m -2 .s -1, the wavelength was 690 nm, the light time was 16 h / d, and the culture temperature was 27°C for 80 days.

[0031] (2) Induce flowering stage I: use 1 / 2 MS as basic medium, add PP 333 1.50 mg / L+ABA 1.0 mg / L, under light intensity of 75 mu mol.m -2 .s -1 , wavelength 690 nm, culture temperature 28 ℃, additional banana juice 20% (v / v), activated carbon 0.5% culture conditions, after 30 days of culture, it entered the induction stage II.

[0032] (3) Induction of flowering stage II: 1 / 2 MS as the basic medium, adding TDZ 1.00 mg / L + BA 0.5 mg / L, at a light intensity of 75 mu mol.m -2 .s -1 , wavelength 590 nm, culture temperature 16°C, additional banana juice 15% (v / v), activated carbon 0.5% culture conditions,...

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Abstract

The invention relates to a method for bloom of dendrobium candidum in a test tube, belonging to the technical field of agricultural plant biology. The method comprises the following steps of: taking protocorm obtained by the isolated culture of dendrobium candidum stems as test material, and culturing strong seedling for 50-80d; and alternately culturing and inducing through blooming inducing stage I, blooming inducing stage II and blooming inducing stage I, and carrying out blooming culturing stage. According to the method for inducing bloom of the dendrobium candidum in the test tube, the formation of the dendrobium candidum buds can be effectively induced, and the blooming rate of the dendrobium candidum test tube can be adjusted and controlled; and the method can induce the strong plant, the flower size is large, the blooming inducing rate is 90.5%-96.7%, and the flower with integrated flower organ reaches up to 98.5%-100%.

Description

technical field [0001] The invention relates to a test-tube flowering method of dendrobium candidum, which belongs to the field of agricultural plant biotechnology. Background technique [0002] Dendrobium officinale ( Dendrobium officinale Kimura et Migo ), also known as black knot grass, is an aerial orchid herb. Dendrobium officinale is the best of Dendrobium, named for its iron-green skin, and is a famous ornamental and medicinal orchid. Because of its harsh requirements on habitat conditions, it is difficult to flower. Under cultivation conditions, it usually takes 3 to 4 years from seed germination to flowering. [1] . The application of plant biotechnology to induce flowering of Dendrobium officinale has the characteristics of simple operation, controllable growth conditions, strong repeatability, and short cycle, and can provide more accurate research methods and good experiments for the study of plant flowering mechanism and physiology. system. Therefore, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘狄赖钟雄陈发兴林玉玲刘生财吴高杰
Owner FUJIAN AGRI & FORESTRY UNIV
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