Dendrobium candidum test-tube flowering and seed setting method

Abstract

The invention discloses a dendrobium candidum test-tube flowering and seed setting method comprising the steps that: dendrobium candidum stems or capsules are selected as explants and are subjected to induced culturing; protocorm roliferation culturing, protocorm rooting culturing, rooted seedling induced flowering culture, a pollination process, and seed setting culturing are carried out, such that test-tube flower and seed are formed. With the method, dendrobium candidum test-tube flowering rate can be accurately and effectively regulated. Also, according to the invention, pollination is carried out in a sterile environment, and seed setting culturing is carried out by using a special culture medium after pollination, such that seed setting rate is improved. A capsule diameter of obtained fruit can reach 0.8-1cm, and a pollination seed setting rate can reach 90%.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for flowering and fruiting of dendrobium officinale test tubes. Background technique [0002] Dendrobium candidum, also known as black knot grass, belongs to the epiphytic orchid class of Orchidaceae, and is a perennial herb. Dendrobium candidum is the best product in Dendrobium nobile. It is named for its iron-green skin. It is a famous ornamental and medicinal orchid; Proliferation, differentiation, rooting culture, and seedlings are obtained, but there are relatively few studies on the flowering and fruiting of test tubes in the later stage; Dendrobium officinale has harsh requirements on the habitat conditions, and it is difficult to flower. Under cultivation conditions, it usually takes 3 to 4 years from seed germination to flowering Therefore, the induction of the flowering of Dendrobium candidum is of great significance to improving the floweri...

AI Technical Summary

Problems solved by technology

Dendrobium candidum is the best product in Dendrobium nobile. It is named for its iron-green skin. It is a famous ornamental and medicinal orchid; Proliferation, differentiation, rooting culture, and seedlings are obtained, but there are relatively few studies on the flowering and fruiting of test tubes in the later stage; Dendrobium officinale has harsh requirements on the habitat conditions, and it is difficult to flower. Under cultivation conditions, it usually takes 3 to 4 years from seed germination to flowering Therefore, the induction of the flowering of Dendrobium candidum is of great significance to improving the flowering mechanism of plants; and when the existing application of plant biotechnology induces flowering of Dendrobium candidum, although a certain flowering induction rate can be obtained, the induction rate is low , high cost, small flowers, and often incomplete flowers

[0003] In addition, Dendrobium candidum is an insect-borne flower, the pollination rate is low under natural conditions, and it is generally artificially pollinated. The existing pollination method is generally: select healthy plants with a diameter of 0.8 to 1.2 cm and excellent flowers with normal structure in sunny days, and pull them out. Use a toothpick or a pin to remove the anthers from the lip and put them into the pistil cavity, and bag the flowers after pollination

However, the above method has the following defects: it is greatly affected by natural environmental factors, and the pollination success rate is low; after pollination, the plant does not receive a separate special nutrient supply, resulting in insufficient nutrients, which directly affects fruit development, resulting in small fruit clips and low seed setting rate.

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