Dendrobium candidum test-tube flowering and seed setting method

A technology of dendrobium candidum and test tube flowering, which is applied in the field of bioengineering, can solve the problems of low pollination success rate, low pollination rate, and harsh habitat conditions, and achieve the effects of high pollination and seed setting rate, increased flowering rate, and improved seed setting rate

Inactive Publication Date: 2014-08-27
湖北襄草源生态农业科技有限公司
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AI Technical Summary

Problems solved by technology

Dendrobium candidum is the best product in Dendrobium nobile. It is named for its iron-green skin. It is a famous ornamental and medicinal orchid; Proliferation, differentiation, rooting culture, and seedlings are obtained, but there are relatively few studies on the flowering and fruiting of test tubes in the later stage; Dendrobium officinale has harsh requirements on the habitat conditions, and it is difficult to flower. Under cultivation conditions, it usually takes 3 to 4 years from seed germination to flowering Therefore, the induction of the flowering of Dendrobium candidum is of great significance to improving the flowering mechanism of plants; and when the existing application of plant biotechnology induces flowering of Dendrobium candidum, although a certain flowering induction rate can be obtained, the induction rate is low , high cost, small flowers, and often incomplete flowers
[0003] In addition, Dendrobium candidum is an insect-borne flower, the pollination rate is low under natural conditions, and it is generally artificially pollinated. The existing pollination method is generally: select healthy plants with a diameter of 0.8 to 1.2 cm and excellent flowers with normal structure in sunny days, and pull them out. Use a toothpick or a pin to remove the anthers from the lip and put them into the pistil cavity, and bag the flowers after pollination
However, the above method has the following defects: it is greatly affected by natural environmental factors, and the pollination success rate is low; after pollination, the plant does not receive a separate special nutrient supply, resulting in insufficient nutrients, which directly affects fruit development, resulting in small fruit clips and low seed setting rate.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] In May, choose Guangnan red-stem soft-footed Dendrobium candidum, choose the stem section of Dendrobium candidum with a length of 1.5 cm between the stem nodes that grows well and has no obvious diseases and insect pests as explants, cut off the leaves and rinse under tap water After 10 minutes, take it out and put it in the sterilization tray on the ultra-clean workbench; place the gun-shaped tweezers and scalpel in the inoculation instrument sterilizer and heat to 300°C for sterilization; Cut to obtain the stem section with leaf buds, put the stem section in a sterile bottle, sterilize it with 75% ethanol solution for 1min, pour out the ethanol, and then cut off excess tissues such as petiole and leaf sheath with a scalpel, and use 0.1 % mercuric chloride for 6 minutes, and then rinsed with sterile water for 3 to 5 times; the sterilized explants were inoculated in the following sterile induction medium: MS+BA1.5mg / L+NAA0.2mg / L+white sugar 30g / L+agar powder 5.5g / L+pot...

Embodiment 2

[0043] Select Dendrobium officinale capsules with a length of 1.5 cm and a width of 1 cm without pests and diseases as explants, rinse them under tap water for 8 minutes, and wipe the fruit clips with 200:1 84 disinfectant (1 part of 84 disinfectant diluted with 200 parts of water) , placed in a sterile bottle on an ultra-clean workbench, sterilized with 75% ethanol solution for 1 min, poured out the ethanol, cut off the capsule stalk and navel with a scalpel, soaked in 0.1% mercury liter for 6 min, and then used Rinse with sterile water for 3 to 5 times; put the gun-shaped forceps and scalpel in the inoculation instrument sterilizer and heat to 300°C for sterilization, cut the sterilized capsules with a scalpel, and take the seeds as explants for later use; This explant is then inoculated in the following aseptic induction medium: MS+BA2.0mg / L+NAA0.6mg / L+white sugar 50g / L+agar powder 8g / L+potato juice 70ml / L+activated carbon powder 0.7g / L, Under the condition that the tempera...

Embodiment 3

[0055] In May, choose Guangnan red-stem soft-footed Dendrobium candidum, from the Dendrobium candidum, choose the stem section of Dendrobium candidum with a length of 2.5cm between the stem nodes that grows well and has no obvious diseases and insect pests as explants, cut off the leaves and rinse under tap water After 10 minutes, take it out and put it in the sterilization tray on the ultra-clean workbench; place the gun-shaped tweezers and scalpel in the inoculation instrument sterilizer and heat to 300°C for sterilization; Cut to obtain the stem section with leaf buds, put the stem section in a sterile bottle, sterilize it with 75% ethanol solution for 1min, pour out the ethanol, and then cut off excess tissues such as petiole and leaf sheath with a scalpel, and use 0.1 % mercuric chloride for 6 minutes, then rinsed with sterile water for 3 to 5 times; the sterilized explants were inoculated in the following sterile induction medium: MS+BA1.8mg / L+NAA0.4mg / L+white sugar 40g ...

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Abstract

The invention discloses a dendrobium candidum test-tube flowering and seed setting method comprising the steps that: dendrobium candidum stems or capsules are selected as explants and are subjected to induced culturing; protocorm roliferation culturing, protocorm rooting culturing, rooted seedling induced flowering culture, a pollination process, and seed setting culturing are carried out, such that test-tube flower and seed are formed. With the method, dendrobium candidum test-tube flowering rate can be accurately and effectively regulated. Also, according to the invention, pollination is carried out in a sterile environment, and seed setting culturing is carried out by using a special culture medium after pollination, such that seed setting rate is improved. A capsule diameter of obtained fruit can reach 0.8-1cm, and a pollination seed setting rate can reach 90%.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for flowering and fruiting of dendrobium officinale test tubes. Background technique [0002] Dendrobium candidum, also known as black knot grass, belongs to the epiphytic orchid class of Orchidaceae, and is a perennial herb. Dendrobium candidum is the best product in Dendrobium nobile. It is named for its iron-green skin. It is a famous ornamental and medicinal orchid; Proliferation, differentiation, rooting culture, and seedlings are obtained, but there are relatively few studies on the flowering and fruiting of test tubes in the later stage; Dendrobium officinale has harsh requirements on the habitat conditions, and it is difficult to flower. Under cultivation conditions, it usually takes 3 to 4 years from seed germination to flowering Therefore, the induction of the flowering of Dendrobium candidum is of great significance to improving the floweri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 林忠全
Owner 湖北襄草源生态农业科技有限公司
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