Porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine and preparation method thereof

Abstract

The invention discloses porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine which is prepared by mixing inactivated porcine parvovirus virus solution and nanometer alumina gel adjuvant according to the ratio of 1 to 2. According to the invention, safety evaluation to the vaccine is performed on suckling mice and pigs, the results show that after immunization, one suckling mouse dies, no sensitization response is caused on a baby pig, as the same, no obvious full body or partial reaction can be observed, so that the inactivated vaccine is fully proved to be safe and reliable. The porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine set has a CD4 + / CD 8 + relative value in the fourth immunization week, which is obviously higher than that of other vaccine set, the relative value is increased to 2.97 from 2.11, which shows that the vaccine enhances the immune state of cells of mice. The porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine provided by the invention can induce the secretory expression of IFN-Gamma, and the vaccine can remarkably enhance the immune response reaction produced by Th 1 type cells, to induce the Th 1 type cells to secrete IFN- Gamma. Therefore, the immune level of cells can be increased, and a stronger cellular immunity response can be produced.

Description

technical field [0001] The invention belongs to the technical field of porcine parvovirus vaccines, in particular to a porcine parvovirus nano-alumina gel adjuvant inactivated vaccine and a preparation method thereof. Background technique [0002] Porcine parvovirus disease is an important sow reproductive disorder disease, which is very harmful to both sows and breeding pigs. Vaccine immunization is the main means to control the disease. Now domestic sows in large-scale pig farms and breeding pigs and reserve breeding pigs in pig farms all need to be immunized against the disease. Inactivated vaccines are commonly used to prevent PPV, and they have many advantages such as safety and long-acting effect. However, the conventional white oil adjuvant vaccines cannot produce a good cellular immune response after immunizing the body. Conventional aluminum adjuvants have the disadvantages of occasional rash, subcutaneous nodules, contact allergy and other symptoms at the injectio...

AI Technical Summary

Problems solved by technology

As a common vaccine for preventing PPV, the inactivated vaccine has many advantages such as safety and long-term effect, but the conventional white oil adjuvant vaccine cannot produce a good cellular immune response after immunizing the body

Conventional aluminum adjuvants have the disadvantages of occasional rash, subcutaneous nodules, contact allergy and other symptoms at the injection site and increased sensitivity of susceptible individuals

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