Porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine and preparation method thereof

A parvovirus, inactivated vaccine technology, applied in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of increasing the sensitivity of susceptible individuals, contact allergies, and occasional rashes, etc. Achieve the effect of enhancing the level of cellular immunity, enhancing the immune response, and strengthening the cellular immune response

Active Publication Date: 2012-07-18
HENAN AGRICULTURAL UNIVERSITY
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a common vaccine for preventing PPV, the inactivated vaccine has many advantages such as safety and long-term effect, but the conventional white oil adjuvant vaccine cannot produce a good cellular immune response after i

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine and preparation method thereof
  • Porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine and preparation method thereof
  • Porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The porcine parvovirus nano-aluminum adjuvant inactivated vaccine is prepared by mixing the inactivated porcine parvovirus liquid and the nano-aluminum adjuvant at a ratio of 1:2.

[0028] The preparation method of described porcine parvovirus nano aluminum gel adjuvant inactivated vaccine, its steps are as follows:

[0029] (1) Increase, purify and inactivate porcine parvovirus;

[0030] (2) To prepare nano-aluminum adjuvant, dissolve aluminum chloride in distilled water, the mass ratio of aluminum chloride to distilled water is 1:20-30, to make aluminum chloride solution, and the mass concentration fraction is 25-28% Drop ammonia water into the aluminum chloride solution, adjust the pH to 9.0, and stir for 10-15 minutes to obtain a white precipitate, which is aluminum hydroxide gel. Then, the aluminum hydroxide gel precipitate is collected by ultracentrifugation, washed with water, and then transferred to In the Erlenmeyer flask, add distilled water to make a suspens...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine which is prepared by mixing inactivated porcine parvovirus virus solution and nanometer alumina gel adjuvant according to the ratio of 1 to 2. According to the invention, safety evaluation to the vaccine is performed on suckling mice and pigs, the results show that after immunization, one suckling mouse dies, no sensitization response is caused on a baby pig, as the same, no obvious full body or partial reaction can be observed, so that the inactivated vaccine is fully proved to be safe and reliable. The porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine set has a CD4 +/CD 8 + relative value in the fourth immunization week, which is obviously higher than that of other vaccine set, the relative value is increased to 2.97 from 2.11, which shows that the vaccine enhances the immune state of cells of mice. The porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine provided by the invention can induce the secretory expression of IFN-Gamma, and the vaccine can remarkably enhance the immune response reaction produced by Th 1 type cells, to induce the Th 1 type cells to secrete IFN- Gamma. Therefore, the immune level of cells can be increased, and a stronger cellular immunity response can be produced.

Description

technical field [0001] The invention belongs to the technical field of porcine parvovirus vaccines, in particular to a porcine parvovirus nano-alumina gel adjuvant inactivated vaccine and a preparation method thereof. Background technique [0002] Porcine parvovirus disease is an important sow reproductive disorder disease, which is very harmful to both sows and breeding pigs. Vaccine immunization is the main means to control the disease. Now domestic sows in large-scale pig farms and breeding pigs and reserve breeding pigs in pig farms all need to be immunized against the disease. Inactivated vaccines are commonly used to prevent PPV, and they have many advantages such as safety and long-acting effect. However, the conventional white oil adjuvant vaccines cannot produce a good cellular immune response after immunizing the body. Conventional aluminum adjuvants have the disadvantages of occasional rash, subcutaneous nodules, contact allergy and other symptoms at the injectio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/23A61K39/39A61P31/20
Inventor 魏战勇耿静微宋美荣胡慧王瑞宁王亚宾崔保安
Owner HENAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap