Calyx canthus tissue culture rapid propagation method

Abstract

The invention relates to a calyx canthus tissue culture rapid propagation method, which is realized through the steps such as selection of an explant, successive transfer culture, rooting culture and field domestication. A special culture medium is selected respectively for the successive transfer culture step and the rooting culture step. A reasonable domestication means is adopted, so that the growth of the calyx canthus after being transplanted is fast, the survival rate is high, the propagation coefficient is high, and the propagation effect is good.

Description

technical field [0001] The invention relates to the field of horticulture, in particular to a method for tissue culture and rapid propagation of wintersweet. Background technique [0002] Wintersweet (scientific name: Chimonanthus praecox) is a plant of the genus Chimonanthaceae. Distributed in North Korea, America, Japan, Europe, and Hunan, Fujian, Shandong, Jiangsu, Anhui, Yunnan, Henan, Hubei, Zhejiang, Sichuan, Guizhou, Shaanxi, Jiangxi and other regions in mainland China. It grows at an altitude of 300 meters to 700 meters. areas, often grow in mountain forests. Wintersweet is a shrub with deciduous leaves, and it is also a traditional and precious ornamental flower and tree special in my country. It has a long history of cultivation and rich wintersweet culture. Li Shangyin, a poet in the Tang Dynasty, called the wintersweet plum a cold plum, and there is a sentence "knowing the cold plum and crossing the wild pond". "Yao's Remnant Words" is also called plum as a co...

AI Technical Summary

Problems solved by technology

[0004] At present, Wintersweet is a greening tree species widely used in gardens. The common propagation methods are cutting, grafting and layering. There are few studies on tissue culture and rapid propagation.