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Method suitable for rapid rooting of blueberry tissue culture seedlings

A technology for tissue culture seedlings and blueberries, which is applied to the field of rapid rooting of blueberry tissue culture seedlings, can solve the problems of complex rooting procedures, long rooting period, low rooting rate of blueberries, etc., and achieves the effects of shortening the seedling raising period, simple operation and low cost.

Inactive Publication Date: 2012-08-01
ZHEJIANG NORMAL UNIVERSITY
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Problems solved by technology

However, the blueberry rooting stage reported in the existing literature has problems such as low rooting rate, long rooting cycle, complicated rooting procedures and low survival rate of transplanted seedlings, making it impossible for large-scale industrial production

Method used

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Embodiment Construction

[0014] A method suitable for rapid rooting of blueberry tissue culture seedlings, comprising the following steps: 1), put a small amount of pebbles in a tissue culture bottle, then put a nutrient bowl, and seal it with tissue culture sealing film; 2), put blueberry group After the seedlings are soaked with the rooting agent, the cuttings and rooting cultures are carried out in the nutrient pots in the bottle to obtain rooting seedlings; 3), the blueberry tissue culture seedlings grown on the rooting substrate for 40-50 days and reaching more than 6-8cm are grown by single plant After cutting and soaking the rooting agent, the cuttings were inserted into the nutrition pot in the bottle, and several root systems were grown from the seedling base in 18-22 days; in this example, several root systems were grown from the seedling base in 20 days. 4), growing the rooting tissue culture seedlings above 6-8cm in step 3), uncovering the tissue culture sealing film, refining the seedlings...

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Abstract

The invention relates to a method suitable for rapid rooting of blueberry tissue culture seedlings, which includes the steps: cutting off single blueberry tissue culture seedlings which are cultured on rooting medium for 40-50 days and grow to more than 6-8cm, soaking the seedlings in rooting agent, performing cuttage to insert cuttings into feeding block in each bottle, and allowing the seedlings to grow a plurality of roots at the base in 18-22 days; removing a tissue culture seal film from the rooted tissue culture seedlings growing to more than 6-8cm, exercising the seedlings for 30 days, and directly transplanting the seedlings to a greenhouse. By the method, blueberry rooting culture is performed using the feeding blocks in the bottles initially, proper rooting medium and best rooting agent are selected, and accordingly an efficient blueberry tissue culture seedling rooting system is established. The method has the advantages that operation is simple, cost is low, the method is stable and efficient, seedling cycle is shortened greatly, and the like.

Description

technical field [0001] The invention relates to the technical field of plant vegetative propagation, in particular to a method suitable for rapid rooting of blueberry tissue culture seedlings. Background technique [0002] Blueberry (Vaccinium corymbosum) is a bilberry plant of the Rhododendron family, a perennial deciduous or evergreen shrub with berries as its fruit. Nutritious health fruit with health effects and medicinal value. Blueberries are rich in antioxidant compounds such as various vitamins, arbutin, anthocyanins, SOD, tanning acids and flavonoids, so they have unique health care functions. Blueberry is also one of the most promising fruit tree species in the world in this century. [0003] Blueberry fruit is not only attractive in color, but also has delicate flesh, moderate sour and sweetness, rich nutrition, refreshing aroma and unique flavor. According to reports, blueberries have the highest content of antioxidants in all fruits and vegetables, especially...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈文荣杨琴郭卫东余柯达李晓洁
Owner ZHEJIANG NORMAL UNIVERSITY
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