Application of oleanolic acid in pharmacy

Abstract

The invention discloses an application of oleanolic acid in pharmacy, and relates to the application of the oleanolic acid in the preparation of the medicine for restraining the activation of kidney fibroblast and mesangial cell, the medicine for deducing the kidney fibroblast, the medicine for improving the urine protein, the medicine for improving the kidney function, the medicine for reducing the kidney inflammation and the collagen deposition, and the medicine for reducing gene and protein related to the index of the kidney fibrosis. The invention designs a screening platform for the activation of the TGF (transforming growth factor)-beta 1-induced rat kidney fibroblast and mesangial cell, and discovers that the oleanolic acid with 4.56*10<-5>g/ml-4.56*10<-6>g/ml can restrain the activation of the TGF-beta 1-induced rat kidney fibroblast and mesangial cell. The invention further designs an in-vivo experiment of the oleanolic acid to UUO (unilateral ureteral obstruction) model rat and Platt model rat, and discovers that the oleanolic acid with the middle dose (6mg/kg/d) and the high dose (7mg/kg/d) can reduce the gene and the protein related to the urine protein, the kidney function and the kidney fibrosis of the UUO model rat and the Platt model rat.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine and relates to the use of oleanolic acid, in particular to the application of oleanolic acid in pharmacy. Background technique [0002] Renal fibrosis, characterized by glomerulosclerosis and tubulointerstitial fibrosis, is the final outcome of many chronic kidney diseases. Essentially, renal fibrosis is a process characterized by excessive accumulation and deposition of extracellular matrix. Activation of matrix-producing cells is key to the development of renal fibrosis. Under physiological conditions, fibroblasts produce an appropriate amount of collagen matrix to fill the space between nephrons, blood vessels and the renal capsule. The volume of the kidney gradually increases with age, and fibroblasts mainly play a role in remodeling the renal interstitium. When injury and inflammatory factors persist, fibroblasts proliferate and produce excess extracellular matrix. Newly synthe...

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Problems solved by technology

[0006] At present, the role of oleanolic acid in inhibiting the activation of renal fibroblasts and mesangial cells, reducing urinary protein in UUO model and Platt model rats (24h urine protein quantification, α1-MG, NAG, β2-MG, TRF), Renal function (Scr, BUN), reducing renal inflammation and collagen deposition, and improving renal fibrosis-related genes and proteins (TGF-β1, CTGF, Collagen III, LN, Collagen I, FN) have not yet been reported

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