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Method for building spleen-deficiency chronic atrophic gastritis gastric precancerous lesions animal model

A technology of atrophic gastritis and gastric precancerous lesions, applied in the establishment of animal models of gastritis and gastric precancerous lesions, and in the field of establishment of animal models of chronic atrophic gastritis with gastric precancerous lesions due to spleen deficiency, achieving reproducible and stable results

Inactive Publication Date: 2012-08-29
潘华峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] To sum up, free drinking of MNNG is a mature and feasible method for replicating the CAG rat model, and gas consumption, broken gas and abnormal hunger and fullness are relatively mature and feasible methods for replicating the rat model of spleen deficiency syndrome. The rat model of gastric precancerous lesions of gastritis, and how to determine the model time to ensure that the animals are in the precancerous lesions of the stomach (PLGC, intestinal metaplasia and atypical hyperplasia) have not been reported yet

Method used

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  • Method for building spleen-deficiency chronic atrophic gastritis gastric precancerous lesions animal model
  • Method for building spleen-deficiency chronic atrophic gastritis gastric precancerous lesions animal model
  • Method for building spleen-deficiency chronic atrophic gastritis gastric precancerous lesions animal model

Examples

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Effect test

Embodiment 1

[0020] Example 1 Prepare MNNG with sterilized tap water to make a storage solution with a concentration of 10 mg / L, store it in a dark place and refrigerate it, make it into a drinking solution at 80 μg / mL, put it into a 200-mL drinking bottle instead of drinking water (with black light-proof paper on the outside, and The whole preparation process was protected from light), and the rats were allowed to drink freely; after the 5th week of free drinking of the MNNG solution, fasting was started every other day, that is, no fasting on one day, fasting on the next day (no water), and no fasting on the next day , so repeatedly; after the MNNG solution was free to drink, in the 7th week, Xiaochengqi Decoction was added to the gavage of 1ml / monkey / d, 1 time / d, and the modeling was continued for 12 weeks.

Embodiment 2

[0021] Example 2 The prepared 10 mg / L concentration of MNNG storage solution was made into 150 μg / mL drinking solution, and put into a 200mL drinking water bottle instead of drinking water (black light-proof paper was attached to the outside, and the whole preparation process was protected from light). The rats were free to drink; after the 5th week of free drinking of the MNNG solution, fasting was started every other day, that is, no fasting on one day, fasting on the next day (no water), and no fasting on the next day, and so on; after free drinking of the MNNG solution In the 7th week, 2ml of Xiaochengqi decoction was added to the rats by gavage, once a day, and the model was established continuously for 12 weeks.

Embodiment 3

[0022] Example 3 The prepared 10 mg / L concentration of MNNG storage solution was made into 200 μg / mL drinking solution, and put into a 200mL drinking bottle instead of drinking water (black light-proof paper was attached to the outside, and the whole preparation process was protected from light). The rats were free to drink; after the 5th week of free drinking of the MNNG solution, fasting was started every other day, that is, no fasting on one day, fasting on the next day (no water), and no fasting on the next day, and so on; after free drinking of the MNNG solution In the 7th week, 3ml of Xiaochengqi decoction was added to the rats by gavage, once a day, and the model was established continuously for 12 weeks.

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Abstract

The invention relates to a method for building a spleen-deficiency chronic atrophic gastritis gastric precancerous lesions animal model. The method is based on the existing spleen-deficiency rat model and the existing chronic atrophic gastritis rat model and is used for building the animal model through united copy of free drinking of N-methyl-N '-nitro-N -nitrosoguanidine (MNNG) solution, irregular eating and purgation. The method comprises the following specific steps of: preparing 10mg / L MNNG storage solution by utilizing sterilized tap water, preparing 80 to 200 mug / mL MNNG solution in temporary use to substitute the drinking water to be drunk freely by the rats, and adopting the free drinking of MNNG as the beginning of the modeling; copying a spleen-deficiency syndrome through irregular eating and improved purgation way, feeding the rats on alternate days since the fifth week after the modeling, and utilizing qi-activating decoction for gavage in a dose of 1 to 3ml per rat every day in the seventh weak; and continuing the modeling for 12 weeks. The animal model built through the method is identical to or similar with the clinical syndrome and physical signs of middle-late lesions in chronic atrophic gastritis. After being verified in repeatedly modeling, the method has good repeatability and good stability. The built model is suitable for selecting traditional Chinese medicine for treating the chronic atrophic gastritis through a traditional Chinese medicine spleen treatment method.

Description

technical field [0001] The invention relates to a method for establishing an animal model of gastric precancerous lesions of gastritis, in particular to a method for establishing an animal model of gastric precancerous lesions of chronic atrophic gastritis with spleen deficiency syndrome, and belongs to the field of basic research and experimental zoology of chronic atrophic gastritis with spleen deficiency syndrome of traditional Chinese medicine. Background technique [0002] Chronic atrophic gastritis (CAG) is a digestive system disease characterized by localized or extensive atrophy, reduction or disappearance of the intrinsic glands of the gastric mucosa, thinning of the mucosal layer, and thickening of the muscularis mucosae. It has a high incidence and is prone to repeated attacks , Difficult to cure. Studies have shown that the incidence of CAG canceration is 10%-19%; and the vast majority of gastric cancer patients have a history of CAG, so CAG is one of the main st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61K49/00
Inventor 潘华峰
Owner 潘华峰
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