Method for building spleen-deficiency chronic atrophic gastritis gastric precancerous lesions animal model

A technology of atrophic gastritis and gastric precancerous lesions, applied in the establishment of animal models of gastritis and gastric precancerous lesions, and in the field of establishment of animal models of chronic atrophic gastritis with gastric precancerous lesions due to spleen deficiency, achieving reproducible and stable results
CN102648701AInactive Publication Date: 2012-08-29潘华峰
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
潘华峰
Publication Date
2012-08-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for building a spleen-deficiency chronic atrophic gastritis gastric precancerous lesions animal model. The method is based on the existing spleen-deficiency rat model and the existing chronic atrophic gastritis rat model and is used for building the animal model through united copy of free drinking of N-methyl-N '-nitro-N -nitrosoguanidine (MNNG) solution, irregular eating and purgation. The method comprises the following specific steps of: preparing 10mg / L MNNG storage solution by utilizing sterilized tap water, preparing 80 to 200 mug / mL MNNG solution in temporary use to substitute the drinking water to be drunk freely by the rats, and adopting the free drinking of MNNG as the beginning of the modeling; copying a spleen-deficiency syndrome through irregular eating and improved purgation way, feeding the rats on alternate days since the fifth week after the modeling, and utilizing qi-activating decoction for gavage in a dose of 1 to 3ml per rat every day in the seventh weak; and continuing the modeling for 12 weeks. The animal model built through the method is identical to or similar with the clinical syndrome and physical signs of middle-late lesions in chronic atrophic gastritis. After being verified in repeatedly modeling, the method has good repeatability and good stability. The built model is suitable for selecting traditional Chinese medicine for treating the chronic atrophic gastritis through a traditional Chinese medicine spleen treatment method.
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Description

technical field

[0001] The invention relates to a method for establishing an animal model of gastric precancerous lesions of gastritis, in particular to a method for establishing an animal model of gastric precancerous lesions of chronic atrophic gastritis with spleen deficiency syndrome, and belongs to the field of basic research and experimental zoology of chronic atrophic gastritis with spleen deficiency syndrome of traditional Chinese medicine. Background technique

[0002] Chronic atrophic gastritis (CAG) is a digestive system disease characterized by localized or extensive atrophy, reduction or disappearance of the intrinsic glands of the gastric mucosa, thinning of the mucosal layer, and thickening of the muscularis mucosae. It has a high incidence and is prone to repeated attacks , Difficult to cure. Studies have shown that the incidence of CAG canceration is 10%-19%; and the vast majority of gastric cancer patients have a history of CAG, so CAG is one of the main st...

Claims

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