Evaluation model for zinc biological effectiveness in foods and establishment method thereof

A bioavailability and evaluation model technology, applied in the field of the establishment of the model, can solve the problems of lack of zinc transport and accumulation process evaluation, differences in bioavailability, etc.

Inactive Publication Date: 2012-09-19
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because this model lacks the evaluation of the transport and accumulation process of zinc to organs in the body, it is different from the abs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Evaluation method of bioavailability of rice grain zinc on human body

[0023] (1) Caco-2 cell culture

[0024] Place the insertion slot in the six-well culture plate. Set the cell density to 3×10 5 1.5 mL of the Caco-2 cell mixture of serotonin was dripped onto the bottom membrane of the insertion groove, and at the same time, 2 mL of DMEM containing 10% fetal bovine serum was added to the basal side;

[0025] (2) 293T cell culture

[0026] Culture human kidney embryo T (293T) cells on the basal side of another six-well plate with the same culture medium as Caco-2 cells;

[0027] (3) Model establishment

[0028] Move the insertion slot with Caco-2 cells into the six-well plate with 293T cells, and co-culture for 24 hours;

[0029] (4) Zinc bioavailability test

[0030] Steam rice grains, grind them, use the traditional digestive system for simulated digestion, centrifuge to obtain the supernatant, mix the supernatant with the MEM solution to make the zinc absorption test...

Embodiment 2

[0034] Example 2 Evaluation method of zinc bioavailability in zinc sulfate and zinc glycinate

[0035] (1) Caco-2 cell culture

[0036] Place the insertion slot in the six-well culture plate. Set the cell density to 3×10 5 1.5 mL of the Caco-2 cell mixture of serotonin was dripped onto the bottom membrane of the insertion groove, and at the same time, 2 mL of DMEM containing 10% fetal bovine serum was added to the basal side;

[0037] (2) 293T cell culture

[0038] Culture human kidney embryo T (293T) cells on the basal side of another six-well plate with the same culture medium as Caco-2 cells;

[0039] (3) Model establishment

[0040] Move the insertion slot with Caco-2 cells into the six-well plate with 293T cells, and co-culture for 24 hours;

[0041] (4) Zinc bioavailability test

[0042] Dissolve zinc sulfate and zinc glycinate in deionized water respectively, use a traditional digestive system for simulated digestion, centrifuge to obtain a supernatant, and mix the supernatant with...

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PUM

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Abstract

The invention relates to an evaluation model for zinc biological effectiveness in foods and an establishment method thereof. The model comprises a model structure, cell types and evaluation indexes, wherein the evaluation indexes are the zinc content in Caco-2 cells, cell transported zinc quantity and the zinc content in epithelial cells of internal organs. The model can accurately, efficiently and comprehensively simulate the zinc absorbing and utilizing process in a human body and provides a reasonable and correct method for evaluating the zinc biological effectiveness in the foods; the model structure is similar to a human intestinal absorption structure; the model can evaluate the absorption efficiency of zinc in the foods in an intestinal tract and also can evaluate the utilization efficiency of the zinc in the human body; and evaluation results are close to the actual zinc biological effectiveness.

Description

Technical field [0001] The invention provides an evaluation model suitable for zinc bioavailability in cereal grains, zinc fortifiers and animal foods, and also relates to a method for establishing the model. Background technique [0002] Dietary diversity, food zinc fortification, and supplementation of zinc fortifiers are important ways to solve human zinc deficiency. However, surveys in recent years have found that there is still a large proportion of the world, especially children with more serious zinc deficiency. A survey conducted in my country in 2008 showed that children whose hair zinc is below the lower limit of normal are still as high as 60%. This result reminds us that we should pay more attention to the actual biological effectiveness of dietary zinc on the human body. Due to ethics and human life safety, the use of human experiments to evaluate the bioavailability of zinc in diets is limited. In recent years, the development of cell culture technology and relate...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/02G01N27/62G01N27/626
Inventor 何万领李晓丽李旺李元晓邓凯伟曹平华
Owner HENAN UNIV OF SCI & TECH
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