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Method for detecting chronic hepatitis B liver fibrosis serum N-glycome peak mark and application thereof

A technology for hepatitis liver fibrosis and fibrosis, applied in the field of biomedicine, can solve problems such as the difficulty of glycan structure research, and achieve the effect of improving specificity and sensitivity

Inactive Publication Date: 2012-10-03
上海交通大学附属第一人民医院
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

It is known that liver cells are the main source of glycoproteins in serum except plasma cells, and the liver also has the function of removing abnormally glycosylated proteins in the body. Therefore, liver lesions can cause changes in serum glycoprotein profiles, but the glycan structure is highly Factors such as complexity have brought many difficulties to research

Method used

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  • Method for detecting chronic hepatitis B liver fibrosis serum N-glycome peak mark and application thereof
  • Method for detecting chronic hepatitis B liver fibrosis serum N-glycome peak mark and application thereof
  • Method for detecting chronic hepatitis B liver fibrosis serum N-glycome peak mark and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1 Serum samples from patients with chronic HBV infection were detected by DSA-FACE technology, and N-glycan profiles with peaks were obtained

[0037] Serum samples from 146 patients with chronic HBV infection who met the criteria and underwent liver biopsy were collected from 2002 to 2007.

[0038] Inclusion criteria: According to the diagnostic criteria for chronic HBV infection stipulated in the "Guidelines for the Prevention and Treatment of Chronic Hepatitis B" formulated by the Chinese Medical Association, those who have a positive history of hepatitis B or HBsAg for more than 6 months, and are still positive for HBsAg and / or HBVDNA ;

[0039] Exclusion criteria: HIV or chronic hepatitis C virus infection, alcohol consumption greater than 30g per day, metabolic or other types of liver damage, patients who have received liver disease treatment, liver biopsy tissue does not meet the standards;

[0040] Liver biopsy pathological diagnosis was used to clar...

Embodiment 2

[0052] Example 2 Using N-glycogroup detection results to predict the degree of liver fibrosis

[0053] According to the serum sample detection method in Example 1, the subject’s serum glycome profile with peaks was obtained, and the receiver operating curve (ROC) of participating in this experiment can compare the diagnostic value of each peak in different grouping situations, and predict chronic The degree of hepatitis B liver fibrosis or predict whether there is early liver cirrhosis;

[0054] When used to predict whether there is significant liver fibrosis (S2-4), the peaks with higher area under the ROC curve (AUROC) are: the 8th peak is 0.648, the 10th peak is 0.640, the 1st peak is 0.624, the 1st peak is 0.624, 2 peaks are 0.609 (eg figure 1 shown);

[0055] When used to predict whether there is severe liver fibrosis (S3-4), the peaks with higher AUROC are: the 2nd peak is 0.702, the 10th peak is 0.687, the 1st peak is 0.682, and the 8th peak is 0.672 (such as figu...

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Abstract

The present invention belongs to the field of bio-medicine, relates to a liver fibrosis serum N-glycome peak mark, and specifically relates to a method for detecting the chronic hepatitis B liver fibrosis serum N-glycome peak mark and the application thereof. The method detects a serum sample of a chronic HBV infected patient by using DSA-FACE technology to obtain a glycome map with peak values. After the peak values are quantified, the non-traumatic chronic hepatitis B liver fibrosis serum N-glycome peak mark is obtained by using the internal reference corrected data. The method of the invention can help to significantly improve the specificity and the sensitivity of non-traumatic diagnosis for chronic hepatitis B liver fibrosis, can help to efficiently predict the degree of liver fibrosis in chronic hepatitis B with the N-glycome peak mark as a non-traumatic alternative indicator, and has clinical significance in reality for prognosis estimation, treatment option and treatment evaluation.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a peak marker of liver fibrosis serum N-glycogroup, in particular to a method for detecting the serum N-glycogroup peak marker of chronic hepatitis B liver fibrosis and its application. Background technique [0002] For a long time, liver biopsy has been the gold standard for determining the etiology of liver diseases and evaluating the degree of liver inflammation, necrosis, and fibrosis. It has important clinical significance for prognosis, treatment selection, and efficacy evaluation. Insufficiencies such as inter-observer bias have caused a lot of inconvenience to clinical application. There is an urgent need to find convenient non-invasive alternative indicators in clinical diagnosis and treatment. In recent years, establishing a diagnostic model of liver fibrosis with a combination of serum indicators has become a worldwide research hotspot. Among a series of non-invasive diag...

Claims

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Application Information

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IPC IPC(8): G01N33/66
Inventor 陆伦根徐铭益曲颖周锟高春芳
Owner 上海交通大学附属第一人民医院
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