Preparation method of protein chip used for screening lung cancer
A protein chip, lung cancer technology, applied in material testing products, measuring devices, instruments, etc., to achieve the effect of high sensitivity and specificity
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[0046] Phage display technology is a new technology for fusion expression of foreign protein or polypeptide gene and phage specific protein gene on its surface. This technology is used to express the patient's abnormal cause as abnormal protein and rely on the Qualcomm of protein chip The principle of quantitative screening and antigen-antibody specific binding is used to determine tumor markers. The technology of combining phage display and protein chip has achieved great success in tumor marker screening. In order to screen early lung cancer markers in smokers, we A T7 phage display cDNA library of smokers’ lung cancer tissues was constructed. The essence is to insert the cDNA fragments from the library into the T7 phage vector, and display the inserted fragments on the phage coat protein as a fusion protein.
[0047] A method for detecting lung tumor markers, the specific steps are as follows:
[0048] 1. Construction of lung cancer tissue cDNAT7 phage display library
[0049] 1....
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