Preparation method of protein chip used for screening lung cancer

Abstract

The invention discloses a preparation method of a protein chip used for screening lung cancer. The preparation method is characterized in that smoker lung cancer tissue T7 bacteriophage is constructedto display a cDNA library, a cDNA fragment in the library is inserted into a T7 bacteriophage carrier, the inserted fragment is displayed on the bacteriophage coat protein with a fusion protein form,an exogenous protein or a polypeptide gene and a bacteriophage specific protein gene are subjected to fusion expression on the surface, through biopanning and phage display technologies, the proteinchip is prepared, the abnormal gene of the patient is subjected to fusion expression to the abnormal protein, and high flux screening of the protein chip and an antigen-antibody specifically binding principle are used for determining a tumor marker. The method can be used for screening primary tumor at an early stage, is capable of screening tumour high-risk population, discriminating benign and malignant tumour, determining a tumour development process, and observing the tumour treatment effect, and performs important effect for tumour recurrence prediction and prognosis.

Description

Technical field [0001] The invention relates to a method for preparing a protein chip for lung cancer screening. Background technique [0002] For a long time, smoking is considered to be the primary risk factor for lung cancer. Therefore, researchers have been exploring the mechanism of lung cancer induced by smoking and trying to find markers that can be used as a predictor of lung cancer risk in smokers. As early as the end of the last century, researchers A study of molecular changes in smoking populations has shown that p53 gene mutation and methylation have shown obvious trends in smokers and those who have smoked but have not been diagnosed with lung cancer, and the degree of change can reach 70% and respectively. 100%, Church et al. also used the tobacco-specific carcinogens in serum to study the risk of lung cancer in smoking population for the first time in 2009. The results showed that NNAL (tobacco methylnitrosamine) is the only one of all the detection indicators. M...

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Problems solved by technology

[0006] To sum up, early detection and early intervention of lung cancer are the key to the effective treatment of lung cancer. Although a large number of studies have

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