Method for measuring water-soluble protein drug terahertz dielectric spectrum by waveform rebuilding technology

Abstract

The invention discloses a method for measuring a water-soluble protein drug terahertz (THz) dielectric spectrum by a waveform rebuilding technology. The method comprises: making use of a transmission-type terahertz time-domain spectrum device to measure the terahertz time-domain spectrum of nitrogen without placing a sample and taking the measured terahertz time-domain spectrum as a reference signal, measuring a terahertz time-domain spectrum signal of a sample pool filled with a protein drug water solution sample and taking the signal as a sample signal, then rebuilding the sample signal with the reference signal and a sample pool three-layer transmission model, finally using a Nelder-Mead optimization method to make the rebuilt sample signal approach the measured sample signal, and converting the obtained sample optical parameters into a terahertz complex dielectric spectrum containing a real part and an imaginary part. The invention can make full use of a multilayer accurate transmission model which is established by an electromagnetic transmission theory and in which terahertz passes through the sample to transmit, avoids the difficulty of converting complex numbers into real numbers in ordinary frequency domain optimization algorithm cost functions, and effectively improves the measurement accuracy of a protein drug water solution sample THz dielectric spectrum.

Description

technical field [0001] The invention belongs to the technical field of terahertz waves, and relates to a method for measuring the terahertz dielectric spectrum of water-soluble protein drugs by using waveform reconstruction technology. Background technique [0002] Terahertz (Terahertz or THz) waves usually refer to electromagnetic waves with a frequency in the range of 0.1 to 10 THz. The energy of photons is about 1 to 10 meV, which is roughly equivalent to the energy of transitions between molecular vibration and rotational energy levels. Most polar molecules such as water molecules and ammonia molecules have a strong absorption of THz radiation, and the transition between the vibrational energy level and rotational energy level of many organic macromolecules (DNA, protein, etc.) is also just in the THz range. Therefore, the THz spectrum (including emission, reflection and transmission spectra) of substances contains rich physical and chemical information, and its absorpti...

AI Technical Summary

Problems solved by technology

However, they generally can only reflect the internal information of a single molecule, and especially lack the ability to directly provide structural and conformational information that play a decisive role in protein function.

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