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Fluorescent brightening agent CBS-X detection kit and preparation method thereof

A technology of fluorescent whitening agent and detection kit, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to identify the type of fluorescent whitening agent, high detection cost, and solvent consumption, so as to reduce errors and detection costs Low, simple pre-processing effect

Active Publication Date: 2014-06-04
GUANGZHOU QUALITY SUPERVISION & TESTING INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the direct ultraviolet light irradiation observation method is only a simple judgment, and cannot identify the type of fluorescent whitening agent; chromatography and chromatography-mass spectrometry have the advantages of high specificity and accurate results, but the disadvantage of these two methods is that the sample The pre-treatment is cumbersome, consumes a lot of solvents, and requires expensive laboratory equipment, and the detection cost is high

Method used

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  • Fluorescent brightening agent CBS-X detection kit and preparation method thereof
  • Fluorescent brightening agent CBS-X detection kit and preparation method thereof
  • Fluorescent brightening agent CBS-X detection kit and preparation method thereof

Examples

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Effect test

Embodiment 1

[0025] The main components of the fluorescent whitening agent CBS-X detection kit described in the present embodiment are as follows:

[0026] 1) Fluorescent whitening agent CBS-X specific antigen working solution;

[0027] Prepared by the following method: 0.186g, that is, 1.0mmol of 2-sulfobenzaldehyde, 0.20g, that is, 1.0mmol of 2-sulfo-5-hydroxy-benzaldehyde and 0.40g, that is, 1.0mmol of fluorescent The whitening agent CBS-X was dispersed in 30mL of dry DMSO, 3mL of piperidine was added, and the microwave was heated at 500 watts for 5min. After the reaction was completed, it was poured into water, and the solid was filtered with suction to obtain the fluorescent whitening agent CBS-X derivative with phenolic hydroxyl groups; Disperse 0.53g, i.e. 1.0mmol of the fluorescent whitening agent CBS-X derivative with phenolic hydroxyl group and 1.01g, i.e. 10mmol of potassium carbonate in 80mL of dry DMF, stir at room temperature for 30min, add 1.2g, i.e. 5mmol of N -(3-Bromopro...

Embodiment 2

[0050] Establishment of an indirect competitive ELISA method.

[0051] The competitive inhibition rate of fluorescent whitening agent CBS-X monoclonal antibody was detected by indirect competition ELISA method, the method is as follows:

[0052] 1) Coat a 96-well ELISA plate with 2 μg / mL fluorescent whitening agent CBS-X specific antigen, 100 μL / well, place in a 4°C refrigerator overnight, and use 250 μL / well blocking solution (5% skimmed milk powder ) after sealing, wash with washing solution and pat dry;

[0053] 2) Add fluorescent whitening agent CBS-X standard solution (concentration: 1×10 5 μg / L, 1×10 4 μg / L, 1×10 3 μg / L, 1×10 2 μg / L, 1×10 1 μg / L, 1×10 0 μg / L) or 50 μL of sample solution, then add 50 μL of fluorescent whitening agent CBS-X specific antibody working solution with a concentration of 2 μg / mL, mix well, and incubate at 37°C for 1 hour;

[0054] 3) After washing and patting dry, add horseradish peroxidase-labeled goat anti-mouse secondary antibody worki...

Embodiment 3

[0064] Kit sensitivity, specificity, accuracy.

[0065] 1. Sensitivity determination.

[0066] A standard working curve for the detection of fluorescent whitening agent CBS-X was established by using the results of the indirect competitive ELISA method in Example 2. Fluorescent whitening agent CBS-X monoclonal antibody at 1μg / L~1×10 5 Good linearity in the range of μg / L, IC 50 =905.1ng / mL, the minimum detection limit is 0.553ng / mL, and the detection range (between 20% and 80% inhibition) is 4.714ng / mL~1520.113μg / mL. The detection limit of paper and plastic products is 0.277ng / cm 2 , the detection limit of food samples is 0.277ng / g, and the detection limit of daily chemical products is 50ng / g.

[0067] 2. Determination of specificity.

[0068] Adopt the indirect competition ELISA method of embodiment 2 to measure fluorescent whitening agent CBS-X structural analogue (biphenyl, 2-vinylbenzenesulfonic acid sodium salt, 2-styrenebenzenesulfonic acid sodium salt, 2-bibiphenyl ...

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Abstract

The invention discloses a fluorescent brightening agent CBS-X detection kit, belonging to the technical field of additive detection. The kit comprises an enzyme label plate coated by a fluorescent brightening agent CBS-X specific antigen and a fluorescent brightening agent CBS-X specific antibody solution, wherein each micropore of the enzyme label plate is coated by the fluorescent brightening agent CBS-X specific antigen having a concentration of 1-3 mug / mL, the concentration of the fluorescent brightening agent CBS-X specific antibody is 1-3 mug / mL, and the dosage ratio of the fluorescent brightening agent CBS-X specific antigen to the fluorescent brightening agent CBS-X specific antibody solution is 1:1. The invention further discloses a preparation method of the detection kit. The detection kit disclosed herein can continuously detect a plurality of samples at a time when the fluorescent brightening agent CBS-X is detected, has the characteristics of convenience, rapidness, sensitivity, and low cost, and is suitable for rapidly and accurately detecting fluorescent brightening agent CBS-X in mass samples.

Description

technical field [0001] The invention relates to an additive detection technology, in particular to an enzyme-linked immunological detection kit for the content of fluorescent whitening agent CBS-X and a preparation method thereof. Background technique [0002] Fluorescent whitening agent CBS-X, the chemical name is 4,4'-bis(2-sodium styryl) biphenyl, which belongs to the bistilbene-biphenyl type optical brightener, and is characterized by high whitening strength , Good resistance to chlorine bleaching and oxygen bleaching, excellent wash fastness and dry and wet light exposure, good leveling performance, suitable for washing products, and can also be directly used for optical brightening of textiles and paper. [0003] For a long time, the safety of fluorescent whitening agents has been controversial. Some data show that fluorescent whitening agents are low-toxicity, and some data show that fluorescent whitening agents have a stable molecular structure and are not easy to be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N33/577G01N33/545
Inventor 冼燕萍郭新东罗海英侯向昶吴玉銮邓龙罗东辉柯振华
Owner GUANGZHOU QUALITY SUPERVISION & TESTING INST
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