Bacillus sphaericus parasporal crystal protein preparation method and application thereof
A technology of parasporal crystal protein and Bacillus sphaericus, which is applied in the field of microbial metabolites and its application, can solve the problems of enhancing the toxicity of BinA protein, and achieve the effects of improving activity, high efficacy, and increasing production
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[0036] The method for preparing Bacillus sphaericus parasporal crystal protein and its application provided by the present invention, through batch fermentation of wild-type Bacillus sphaericus, the cells are lysed and centrifuged to obtain its metabolite parasporal crystal protein. The protein is applied to anti-human tumor cells, and the result shows that it can be applied to the preparation of anti-tumor drugs. The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.
[0037] 1. Bacillus sphaericus IAB 872 strain:
[0038] Bacillus sphaericus IAB 872 is an existing Bacillus sphaericus that produces a toxin that is commonly used to kill mosquitoes. Specifically, the present invention is specifically described by a strain of Bacillus sphaericus IAB 872 isolated from a soil sample. Of course, those skilled in the art can also use other channels to obtain B...
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