Method for extracting isoflavone in soybean germ with aid of extrusion and expansion pretreatments

A technology of soybean isoflavones and soybean germ, which is applied in the field of separation and purification of natural chemical substances, can solve the problems of time-consuming operation, high cost, and large amount of solvent, and achieve the effect of simple equipment, good product quality, and improved removal rate

Active Publication Date: 2012-12-26
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

However, the traditional organic solvent extraction method or extraction under heating conditi

Method used

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  • Method for extracting isoflavone in soybean germ with aid of extrusion and expansion pretreatments
  • Method for extracting isoflavone in soybean germ with aid of extrusion and expansion pretreatments
  • Method for extracting isoflavone in soybean germ with aid of extrusion and expansion pretreatments

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experiment example 1

[0041] The following are examples and test data thereof, etc., but the content of the present invention is not limited to the scope of these examples. Experimental example 1 Extrusion pretreatment assisted extraction of soybean isoflavones, screening test of optimal process parameters for extrusion and enzymatic degreasing

[0042] 1. Materials and methods

[0043] 1.1 Materials and reagents

[0044] Soybean Germ Shandong Jiahua Oil Co., Ltd.

[0045]Absolute Ethanol Tianjin Tianli Chemical Reagent Co., Ltd.

[0046] Macroporous Adsorbent Resin Hangzhou Zhengguang Chemical Factory

[0047] Protex6L Alkaline Protease Genencor Bioengineering Co., Ltd.

[0048] All reagents used were analytically pure

[0049] 1.2 Main instruments and equipment

[0050] Extrusion extruder Northeast Agricultural University

[0051] Ultrasonic cell pulverizer Ningbo Xinzhi Biotechnology Co., Ltd.

[0052] Low-speed centrifuge Anhui Zhongke Zhongjia Scientific Instrument Co., Ltd.

[0053] ...

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Abstract

The invention relates to a method for extracting isoflavone in soybean germ with the aid of extrusion and expansion pretreatments. The method comprises the following steps of: adjusting the material water content of soybean germ powder to 5-10%, and performing extrusion and expansion on the soybean germ by using a double-screw extruder, wherein the double-screw extruder has the sleeve temperature of 25-45 DEG C, the screw rotation speed of 60-120rpm and the die orifice aperture of 5-25mm; mixing an expansion product with water, adding protease to carry out enzymolysis, and performing centrifugal separation after the enzymolysis to obtain soybean oil, hydrolysate and dregs, wherein the enzymolysis is carried out under the conditions as follows: enzymolysis pH value: 8-10, enzymolysis temperature: 20-60 DEG C, enzyme adding amount: (1:3)-(1:7), material solution ratio: (1:3)-(1:7), and enzymolysis time:3-7h; adding absolute ethyl alcohol in the hydrolysate and the dregs, extracting isoflavone by ultrasonic waves, wherein the volume ratio of the absolute ethyl alcohol to the hydrolysate is (1-5):1; purifying isoflavone by using macroreticular resin, and eluting to obtain eluate; and rotatably evaporating the eluate, and freezing to be dry to obtain isoflavone. The method provided by the invention has the following characteristics of simple steps, high quality of isoflavone products and high extracting rate.

Description

technical field [0001] The invention relates to a method for extracting soybean isoflavones, in particular to a method for assisting the extraction of soybean isoflavones in soybean germ by extrusion pretreatment, and belongs to the field of separation and purification of natural chemical substances. Background technique [0002] Soybean isoflavones are secondary metabolites formed during the growth of soybeans. They are bioflavonoids, mainly referring to a mixture of polyphenolic compounds with 3-phenylpropanone as the core, which cannot be synthesized in the human body. The distribution in nature is limited, and it mainly exists in leguminous plants, and soybean has the highest content, so it is called soybean isoflavones. Studies have shown that soy isoflavones have a variety of physiological functions and medicinal value. Pharmacological studies have shown that soy isoflavones have estrogen-like effects, which can be used to improve and treat menopausal syndrome in women...

Claims

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Application Information

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IPC IPC(8): C07D311/36C07D311/40
Inventor 江连洲李杨齐宝坤刘珊王中江王胜男
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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