Application of A-type kreotoxin heavy chains to exciting regeneration of neurite

A technology that stimulates nerves and botulinum toxin, which is applied to nervous system diseases, medical preparations containing active ingredients, peptide/protein components, etc., can solve the problems of loss of function and low regeneration ability of nerve cells, and achieve non-toxic side effects, preparations The effect of reliable source and broad market prospect

Inactive Publication Date: 2014-11-26
SHANXI MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] After the central nervous system is injured, the regeneration ability of nerve cells is extremely low, so they often cannot fully regenerate and restore their original functions. Even if the nerve cells themselves survive, they cannot form new synaptic relationships with surrounding cells after their neurite breaks. The function is permanently lost, so finding new drug agents that can stimulate the regeneration of central neurons and neurites is one of the important tasks in the medical field

Method used

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  • Application of A-type kreotoxin heavy chains to exciting regeneration of neurite
  • Application of A-type kreotoxin heavy chains to exciting regeneration of neurite
  • Application of A-type kreotoxin heavy chains to exciting regeneration of neurite

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Embodiment Construction

[0019] A non-limiting example prepared according to the invention is given below.

[0020] A commercially available botulinum toxin type A heavy chain preparation will be used as a stimulator to promote neurite regeneration.

[0021] 1. Recombinant botulinum toxin type A heavy chain product from List Biological Laboratories, USA: product number: 612A, package size: 50 μg. The 436 amino acid sequence of the heavy chain recombination in the product is based on the 872nd to 1296th amino acid sequence of the protoxin. Molecular weight is 51kDa. The recombinant type A botulinum toxin heavy chain still has antigenicity, can react with anti-type A botulinum toxin antibody; and has the ability to bind to GT1b and SV2 on the nerve cell membrane.

[0022] 2. The experimental object used in the present invention is the rodent central nervous system, which comes from the cerebral cortex, hippocampus and spinal cord tissues of embryos or animals 5-10 days after birth. After preparing it...

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Abstract

The invention discloses novel application of A-type kreotoxin heavy chains, and particularly relates to application of A-type kreotoxin heavy chains to exciting regeneration of neurite. A hyperplasia promoting effect of the A-type kreotoxin heavy chains is mainly displayed in that the A-type kreotoxin heavy chains can promote hyperplasia of neurite of motor neurons of fetal spinal cords, can promote regeneration of neurite of motor neurons of spinal cords of mice of five to seven-day old, can promote extension of the neurite of the motor neurons, and can obviously increase the quantity of the neurite of the motor neurons. Accordingly, the A-type kreotoxin heavy chains has the ability of exciting regeneration of neurite of motor neurons of spinal cords of central nervous systems, and can hopefully become an option for developing new drugs and preparations for treating regeneration of injured central nervous systems.

Description

technical field [0001] The invention relates to the application of type A botulinum toxin heavy chain in the preparation of a reagent for promoting and stimulating neurite regeneration. Background technique [0002] Botulinum Neurotoxin (BoNT) is a bacterial exotoxin produced and released by obligate anaerobic Clostridium botulinum. It is a highly pathogenic natural biological toxin. According to its serological characteristics, there are 7 different types of botulinum toxin, namely serotypes A, B, C, D, E, F and G, among which serotype A has the strongest toxicity and is currently the main commercially available botulinum toxin. [0003] The chemical composition of botulinum toxin type A is protein polypeptide, and its molecule is composed of a heavy chain (heavy chain-HC, molecular weight 100kDa) and a light chain (light chain-LC, molecular weight 50kDa), both of which are separated by disulfide bonds interconnected. The type A botulinum toxin that enters the body mainl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P25/02A61P25/28
Inventor 李夏青王志茹王晨亮张宏齐超
Owner SHANXI MEDICAL UNIV
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