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High-efficiency bioflocculant producing bacterium, screening method thereof and application of high-efficiency bioflocculant producing bacterium to treatment of sulfamethoxazole

A biological flocculant and bacteria-producing technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, flocculation/sedimentation water/sewage treatment, etc., can solve the problems of low removal ratio and frequent detection, and reduce production capacity consumption, good removal capacity, increased production and flocculation efficiency

Active Publication Date: 2013-01-16
江苏哈宜环保研究院有限公司
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AI Technical Summary

Problems solved by technology

The antibiotic drug sulfamethoxazole is a typical PPCPs, its removal rate in sewage treatment plants is very low, and it is frequently detected

Method used

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  • High-efficiency bioflocculant producing bacterium, screening method thereof and application of high-efficiency bioflocculant producing bacterium to treatment of sulfamethoxazole
  • High-efficiency bioflocculant producing bacterium, screening method thereof and application of high-efficiency bioflocculant producing bacterium to treatment of sulfamethoxazole
  • High-efficiency bioflocculant producing bacterium, screening method thereof and application of high-efficiency bioflocculant producing bacterium to treatment of sulfamethoxazole

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1, a high-efficiency bioflocculant-producing bacterium is Klebsiella MFX ( Klebsiella sp.), was deposited on June 20, 2012 in the "General Microbiology Center of China Committee for the Collection of Microorganisms", and its preservation number is CGMCC NO. 6243.

[0023] Its screening method is:

[0024] (1) Take 15 mL of fresh activated sludge from Harbin Taiping Sewage Treatment Plant and add it to 100 mL of YP liquid basal medium in a triangular flask, and place it in a shaker with a temperature of 30 °C and a rotation speed of 150 r / min for 18~ After 48 h, the enriched bacterial solution was obtained;

[0025] (2) Take 15mL of the enriched bacteria solution and add it to a 100mL YP liquid basal medium conical flask, repeat the culture conditions of step (1) 3 to 4 times to obtain the acclimatized bacteria solution;

[0026] (3) Gradual dilution of the domesticated bacteria solution, the dilution gradient is 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 ...

Embodiment 2

[0032]In Example 2, the dry powder of the biological flocculant produced by the fermentation of the strains in Example 1 was dissolved in distilled water to prepare a 2 g / L aqueous solution and put it into 1 mg / L sulfamethoxazole sewage. Methods The dosage of flocculant was 1mL, 3mL, 5mL, 7mL and 9mL; the dosage of coagulant aid CaCl2 solution was 0 mL, 0.5 mL, 1 mL, 1.5 mL and 2 mL; 6, 7, 8; the temperature was set to 5°C, 15°C, 25°C, 35°C, 45°C, 55°C, the rotation speed of the shaker was 140 r / min, and the reaction was 0 h, 0.25 h, 0.5 h, 0.75 h, 1 h, 2 h, 4 h, 6 h, 8 h, 10 h, 12 h, the results are as follows Figure 1-Figure 5 As shown, the optimal removal conditions are as follows: pH value is 5, flocculant dosage is 5 mL, coagulation aid dosage is 0.5 mL, flocculation time is 1 h, and temperature is 35 °C. Under these conditions, the removal effect of sulfamethoxazole solution is good, and the removal rate can reach 67.82%.

[0033] To treat the actual domestic sewage c...

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Abstract

The invention discloses a high-efficiency bioflocculant producing bacterium, relates to removal of a trace amount of pollutants which are produced in the city water supply and sewage treatment processes, and solves the problems of low yield of bioflocculant, low artificial regulation performance and single treatment substrate. A screening method comprises the following steps of: domesticating activated sludge of a municipal sewage plant in the Harbin, and enriching biological bacteria, diluting and culturing by using a shake flask, performing plate streaking, separating and purifying biological bacterium species, and repeatedly performing three-area streaking for multiple times to obtain the pure bioflocculant producing bacterial strain KlebsiellaSp.. By the bacterial strain, the removal rate of a trace amount of pollutants in municipal sewage can reach 67.82 percent. By the high-efficiency bioflocculant producing bacterium, the removal rate of a trace amount of pollutants in the municipal sewage is improved, and energy consumption in production is reduced.

Description

technical field [0001] The invention relates to a high-efficiency bio-flocculant-producing bacterium, which belongs to the technical fields of biological engineering and environmental engineering. Screening method for high-efficiency bioflocculant-producing bacteria and its application in the treatment of sulfamethoxazole. Background technique [0002] Pharmaceuticals and Personal Care Products (PPCPs), as emerging pollutants in the aquatic environment, have been at the center of much environmental research today. Drug treatment and personal hygiene requirements lead to continuous release of PPCPs into the environment. Although the existence of these emerging pollutants has been known for decades, analytical methods to detect trace concentrations of PPCPs in the environment have only been developed in the last 10–15 years. Although PPCPs are trace substances, the synergistic effects of chemical persistence and antimicrobial properties presented by PPCPs, as well as the inc...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F1/52C12R1/22C02F101/38
Inventor 马放邢洁杨基先李昂庞长泷吴丹魏薇
Owner 江苏哈宜环保研究院有限公司
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