Method for biotransformation of Panax notoginseng (Burk.) F. H. Chen medicinal material by using Monascus purpureus

A technology of biotransformation and monascus, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of reduced bioavailability, poor absorption, and increased gastrointestinal burden

Inactive Publication Date: 2013-01-30
KUNMING UNIV OF SCI & TECH
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  • Abstract
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Problems solved by technology

[0003] The total saponins of Panax notoginseng are poorly absorbed in the gastrointestinal tract, and the saponins only undergo a slight hydrolysis reaction under the action of gastric juice. Under the action of enzymes or bacteria in the intestinal juice, the metabolites gradually break the glycosidic bonds and degrade into rare saponins to truly exert their pharmacological effects. , which not only increases the gastrointestinal burden, but also reduces the bioavailability
[0005] The main function of Panax notoginseng is to prevent and treat stroke, while the culture of Monascus is mainly used to prevent and treat hyperlipidemia and hypercholesterolemia. Both of them are specific drugs for preventing and treating cardiovascular and cerebrovascular diseases, but in terms of indications each with its own characteristics
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Method used

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  • Method for biotransformation of Panax notoginseng (Burk.) F. H. Chen medicinal material by using Monascus purpureus
  • Method for biotransformation of Panax notoginseng (Burk.) F. H. Chen medicinal material by using Monascus purpureus
  • Method for biotransformation of Panax notoginseng (Burk.) F. H. Chen medicinal material by using Monascus purpureus

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Experimental program
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Effect test

Embodiment 1

[0015] Embodiment 1: the method that Monascus carries out biotransformation to Radix Notoginseng medicinal material, concrete operation is as follows:

[0016] (1) Strain preparation: Monascus ( Monascus purpureus ) was inoculated into PPDA slant medium, and cultured at 30 ℃ for 3 days until colonies grew; Monascus strains were obtained from China General Microorganism Culture Collection and Management Center (CGMCC 3.991);

[0017] (2) Panax notoginseng medium: Accurately weigh 3 g of panax notoginseng powder passed through an 80-mesh sieve into a 100 mL Erlenmeyer flask, add 20 mL of water, sterilize at 121 °C for 30 min at natural pH, and obtain the panax notoginseng medium for future use ;

[0018] (3) Liquid fermentation culture: insert the strains prepared in step (1) into the Sanqi medium prepared in step (2), and culture them at 15 and 35 °C at 120 r / min for 4 days to obtain the strains containing Fermentation products of active ingredients;

[0019] (4) Post-treat...

Embodiment 2

[0024] Embodiment 2: the method that Monascus carries out biotransformation to Radix Notoginseng medicinal material, concrete operation is as follows:

[0025] (1) Strain preparation: Monascus ( Monascus purpureus ) was inoculated into PPDA slant medium, and cultured at a constant temperature of 30 °C until colonies grew; Monascus strains were obtained from China General Microorganism Culture Collection Center (CGMCC), preservation number: 3.991;

[0026] (2) Panax notoginseng medium: Accurately weigh 3 g of panax notoginseng powder passed through a 90-mesh sieve into a 100 mL Erlenmeyer flask, add 24 mL of water, sterilize at 121°C for 30 min at natural pH;

[0027] (3) Liquid fermentation culture: insert the strain prepared in step (1) into the notoginseng medium prepared in step (2), and culture at 30°C and 120 r / min for 2 d and 7 d, and the strains containing Fermentation products of active ingredients;

[0028] (4) Post-treatment of liquid fermentation product: filter ...

Embodiment 3

[0032] Embodiment 3: the method that Monascus carries out biotransformation to Radix Notoginseng medicinal material, concrete operation is as follows:

[0033] (1) Strain preparation: Monascus ( Monascus purpureus ) was inoculated into PPDA slant medium, and cultured at a constant temperature of 30 °C until colonies grew; Monascus strains were obtained from China General Microorganism Culture Collection Center (CGMCC), preservation number: 3.991;

[0034](2) Panax notoginseng medium: Accurately weigh 3 g of panax notoginseng powder passed through a 100-mesh sieve into a 100 mL Erlenmeyer flask, add 18 mL of water, sterilize at 121°C for 30 min at natural pH;

[0035] (3) Liquid fermentation culture: insert the strains prepared in step (1) into the notoginseng medium prepared in step (2), and cultivate them at 25°C and 120 r / min for 5 days;

[0036] (4) Post-treatment of the liquid fermentation product: filter the liquid fermentation product cultivated in step (3), wash the f...

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Abstract

The invention discloses a method for biotransformation of Panax notoginseng (Burk.) F. H. Chen medicinal materials by using Monascus purpureus. The method mainly comprises the following operating steps of: 1) preparation of Monascus purpureus strains; 2) preparation of Panax notoginseng (Burk.) F. H. Chen culture medium; and 3) fermentation and culture to obtain fermentation products containing active components, product post-treatment and detection of major efficacy components. By using the method for fermenting the Panax notoginseng (Burk.) F. H. Chen by using the Monascus purpureus provided by the invention and by directly using Panax notoginseng (Burk.) F. H. Chen raw medicinal materials as fermentation substrate, through one-step fermentation process, medicinal components, i.e. Panax notoginseng (Burk.) F. H. Chen and Monascus purpureus are simultaneously obtained, the culture is enabled to have dual effects of the Panax notoginseng (Burk.) F. H. Chen and the Monascus purpureus, the products contain lovastatin which is a lipid-decreasing active component according to experiments, the type and the content of saponin are obviously changed, and rare saponin with higher pharmacological activity is produced.

Description

technical field [0001] The invention relates to a method for biotransformation of notoginseng medicinal materials by monascus, and a product containing active ingredients can be obtained through the method. Background technique [0002] Panax notoginseng[ Panax notoginseng (Burk.) F. H. Chen] is a plant of the Araliaceae genus Panax, mainly produced in Yunnan, and its main active ingredient is Panax notoginseng saponins ( Panax notoginseng saponins, PNS). So far, more than 70 kinds of ginsenosides have been isolated from Panax notoginseng, all of which are dammarane-type tetracyclic triterpene saponins. According to its saponin type, it can be subdivided into two types: 20(S)-protopanaxadiol [20(S)-protopanaxadiol] and 20(S)-protopanaxatriol [20(S)-protopanaxatriol] . Protopanaxadiol saponins include Rb1, Rb2, Rc, Rd, Rh2, Rg3, Rb3, C-K, PPD; protopanaxatriol saponins include Rg1, Rg2, Re, R1, Rh1, F1, PPT. Among the natural saponins, Rg1, Rb1, Rd, R1, Rc, Re are the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12P17/06C12R1/645
Inventor 葛锋武阳阳王艳刘迪秋陈朝银
Owner KUNMING UNIV OF SCI & TECH
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