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Polymerase chain reaction (PCR) system for identification or auxiliary identification of wheat vernalization gene VRN-1

A VRN-A1, VRN-B1 technology, applied in the field of PCR system, can solve the problems of cumbersome detection procedures and high experimental cost, and achieve the effects of strong target, good repeatability and short time consumption

Inactive Publication Date: 2013-02-06
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Claims
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Problems solved by technology

Therefore, to detect the allelic variation composition of the three loci of the vernalization gene VRN-1 in one wheat material, seven consecutive PCR tests are required, the detection procedures are cumbersome, and the experimental cost is high

Method used

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  • Polymerase chain reaction (PCR) system for identification or auxiliary identification of wheat vernalization gene VRN-1
  • Polymerase chain reaction (PCR) system for identification or auxiliary identification of wheat vernalization gene VRN-1
  • Polymerase chain reaction (PCR) system for identification or auxiliary identification of wheat vernalization gene VRN-1

Examples

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Embodiment 1

[0044] Embodiment 1, multiplex PCR detection of wheat vernalization gene VRN-1

[0045] The multiplex PCR primer pair set in this example consists of two primer pairs specific to the VRN-A1 gene, one primer pair specific to the VRN-B1 gene and one primer pair specific to the VRN-D1 gene. The two primer pairs specific to the VRN-A1 gene are primer pair 1 composed of two single-stranded DNAs shown in sequence 1 and sequence 2 in the sequence listing, and two single-stranded DNAs shown in sequence 3 and sequence 4 A primer pair 2 composed of DNA; the primer pair specific to the VRN-B1 gene is a primer pair 3 composed of two single-stranded DNAs shown in sequence 5 and sequence 6 in the sequence listing; the primer pair specific to the VRN-D1 gene One primer pair is primer pair 4 composed of two single-stranded DNAs shown in sequence 7 and sequence 8 in the sequence listing.

[0046] The multiplex PCR primer pair set of this example can simultaneously detect 8 genotypes of the th...

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Abstract

The invention discloses a PCR system for identification or auxiliary identification of a wheat vernalization gene VRN-1 and an application thereof. A primer pair group of the PCR is composed of four primer pairs: the first primer pair is composed of two single-stranded DNAs presented in a first sequence and a second sequence, the second primer pair is composed of two single-stranded DNAs presented in a third sequence and a fourth sequence, the third primer pair is composed of two single-stranded DNAs presented in a fifth sequence and a sixth sequence, and the fourth primer pair is composed of two single-stranded DNAs presented in a seventh sequence and a eighth sequence. Mutual inhibition effects and mispairing do not exist among the primer pairs in the PCR primer pair group, detected species are reliable in result and good in repeatability, the cost is low, the specificity is high, and time for the detection process is short; the PCR system has important significance for detection of winter and spring characteristics of wheat species, improvement of the breeding efficiency and strengthening of selection of breeding high generations; and additionally, a strong target is provided in popularizing and introducing processes of the wheat by using the system.

Description

technical field [0001] The present invention relates to a PCR system for identifying or assisting identification of wheat vernalization gene VRN-1 and its application, in particular to a multiplex PCR primer pair set for identifying or assisting identification of wheat vernalization gene VRN-1 to be tested , kits and applications thereof. Background technique [0002] Vernalization is one of the basic stages of wheat growth and development. The vernalization response of wheat is closely related to its growth period and adaptability, and is one of the main bases to guide wheat regionalization and new variety promotion, and is controlled by vernalization genes. Vernalization genes determine 70-75% of the whole growth cycle of wheat. Studies by Iwaki and Van Beem have shown that there are differences in the vernalization gene composition of wheat varieties in different regions of the world, reflecting the different requirements of different ecological types for the vernalizat...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11A01G1/00
Inventor 穆培源张晓科相吉山张影全桑伟徐红军韩新年聂迎彬崔凤娟邹波
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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