Technical method for promoting tissue culture rooting of alpine rose amethyst

A technique for amethyst and rhododendron, which is applied in the field of plant cultivation and can solve the problems of low survival rate, separation of seed reproduction traits, no reports and the like

Inactive Publication Date: 2013-02-13
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

In addition, during the cultivation process, problems such as low survival rate of alpine rhododendron cuttings and separation of seed reproductive traits were found. Therefore, domestic scientific researchers have been engaged in the research of alpine rhododendron tissue culture technology, and the species that have s

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  • Technical method for promoting tissue culture rooting of alpine rose amethyst

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[0017] For further understanding content of the invention and effect of the present invention, hereby cite the following examples, and the experimental methods used in the examples are conventional methods unless otherwise specified:

[0018] 1. Preparation of aseptic clustering buds: 60 days before the start of the rooting test, a robust and pest-free amethyst plant with bud stems was selected as explants, and the explants were cleaned, disinfected, buds germinated and clustered buds were induced. References for steps: Liu Xiaoqing, Su Jiale, Xiang Liping, et al. Establishment of Tissue Culture and Optimum System for Alpine Rhododendron Stem [J]. Journal of Yangzhou University (Agriculture and Life Science Edition), 2007, 28(3): 91- 94.

[0019] 2. Preparation of medium: Refer to the element dosage in the literature of Lloyd G and McCown B in 1980, halve the dosage of macroelements and trace elements respectively, and keep the dosage of organic elements and iron salts unchang...

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Abstract

The invention discloses a technical method for promoting the tissue culture rooting of alpine rose amethyst. The method comprises the following steps of: cutting off robust sterile cluster buds by scissors on a sterile ultra-clean work table, and inoculating into the following rooting culture medium, namely a 1/2 WPM minimal medium, adding 10mg/L of added NAA (l-naphthylacetic acid), 10g/L of cane sugar, 0.3g/L of activated carbon and 6g/L of agar powder. The method comprises the following steps of: firstly, carrying out 3 days of dark culture, carrying out 7 days of light culture, taking out bud seedlings on the sterile ultra-clean work table, and transferring into the hormone-free 1/2 WPM minimal medium to continuously carry out 30-40 days of light culture. After the method is used, the rooting percentage of the tissue culture seedling can be improved by more than 30%, the rooting percentage of the amethyst tissue culture seedling can be greatly improved, the tissue culture seedling root system treated by the method has thicker and more secondary branches and can be more easily transplanted to be survived.

Description

1. Technical field [0001] The invention belongs to the technical field of plant cultivation, in particular to the field of tissue culture and rapid propagation of woody ornamental plants, and in particular relates to a technical method for promoting tissue culture and rooting of rhododendron alpine amethyst. 2. Background technology [0002] Alpine Rhododendron is a general term for evergreen broad-leaved species and their hybrids in the Rhododendronaceae Rhododendron family. It often grows on high mountains above 1000m above sea level. Amethyst (Rhododendron hybrids'Roseum Elegans') is one of the more popular alpine rhododendrons on the market. It is an evergreen shrub or small tree, up to 3 meters high. The flowering period is from April to May. The flowers are slightly rose-red lilac Purple, funnel-shaped, single flower up to 6cm in diameter, petals with brown patterns, raceme umbels up to 15-30cm in diameter, large and dark leaves, very cold-resistant and heat-resistant,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 何丽斯苏家乐刘晓青李畅陈尚平
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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