Method for marking grass carp otolith by fluorescent substances
A fluorescent substance and marking technology, applied in the fields of application, fish farming, climate change adaptation, etc., can solve problems such as large damage to fish body, unfavorable release operation, and impact on the process of release and recapture, so as to achieve convenient operation and avoid loss rate High and reduce the effect of manpower and material resources input
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[0016] Marking of grass carp:
[0017] (1) Dissolving calcein in water to obtain calcein solution with a concentration of 250ppm;
[0018] (2) Soak the marked grass carp in the solution for 24 hours, and aerobic;
[0019] (3) When the soaking mark is over, put the fish body in clean water for temporary feeding, and feed it normally for 7 to 10 days a day, then take out the otoliths, clean the otoliths with absolute ethanol, remove impurities on the surface of the otoliths, or use xylene to make them transparent The otoliths were then observed under a fluorescence microscope. It can also be kept in clear water for a while, fed normally, and the otoliths can be taken out for observation at different times. figure 1 It is the marked picture of otoliths of grass carp after soaking for 6 months, A is the control picture, and B is the marked picture; all pictures were taken under the blue excitation light.
[0020] When using the salt calcein solution to soak the grass carp to be...
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