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msCT-CTx fusion protein transgenic engineering strain

A technology of transgenic engineering and fusion protein, applied in the field of fusion protein transgenic engineering strains, can solve problems such as inconvenience for patients, achieve the effects of less psychological burden, eliminating pain, and quick recovery

Inactive Publication Date: 2013-03-13
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] If the patient takes multiple drugs to treat osteoporosis and relieve pain, drug antagonistic reactions are likely to occur; to stagger the time of taking drugs, on the one hand, the half-life of the drug must be considered, and on the other hand, the effective concentration of the drug must also be considered, which will cause inconvenience to the patient
[0005] There is currently no drug that can effectively treat osteoporosis and relieve pain at the same time

Method used

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  • msCT-CTx fusion protein transgenic engineering strain
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  • msCT-CTx fusion protein transgenic engineering strain

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Experimental program
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specific Embodiment approach 1

[0018] Specific embodiment 1: In this embodiment, the msCT-CTx fusion protein transgenic engineering strain is named Escherichia coli BL21 (DE3-msCT / CTx), and Escherichia coli BL21 (DE3-msCT / CTx) is prepared according to the following steps:

[0019] 1. Digest the plasmid vector pUC (msCT / CTx) containing the msCT-CTx fusion protein gene with BamH I and EcoRI to obtain the DNA fragment of the msCT-CTx fusion protein;

[0020] 2. Digest plasmid pGEX-6P-1 with BamHI and EcoRI;

[0021] 3. The DNA fragment of the msCT-CTx fusion protein was enzyme-ligated to the vector pGEX-6P-1 after double enzyme digestion, and then placed at 16°C for overnight connection to obtain the vector pGEX-msCT / CTx;

[0022] 4. Transform Escherichia coli BL21 (DE3) with vector pGEX-msCT / CTx, and select positive recombinants to obtain msCT-CTx fusion protein transgenic engineered bacteria Escherichia coli BL21 (DE3-msCT / CTx).

[0023] Step 4 of this embodiment transforms Escherichia coli BL21 (DE3) by el...

specific Embodiment approach 2

[0024] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that: in step 1, the plasmid vector pUC (msCT / CTx) digestion reaction system is

[0025]

[0026] Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0027] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the enzyme digestion reaction system of plasmid pGEX-6P-1 in step three is

[0028]

[0029] Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses an msCT-CTx fusion protein transgenic engineering strain and relates to a fusion protein transgenic engineering strain. The defect that no medicaments for simultaneously effectively treating osteoporosis and reliving pain exist in the prior art can be overcome. Bacillus coli BL21 (DE3-msCT / CTx) of the msCT-CTx fusion protein transgenic engineering strain is prepared according to the following steps of: 1) obtaining a fusion protein DNA (deoxyribonucleic acid) fragment; 2) performing double digestion on a plasmid; 3) performing enzyme linkage; and 4) transforming the bacillus coli BL21. The msCT-CTx fusion protein transgenic engineering strain disclosed by the invention can be used for the field of pharmaceutical preparation.

Description

technical field [0001] The invention relates to a fusion protein transgenic engineering strain. Background technique [0002] The incidence of osteoporosis in the elderly is high. There are 200 million osteoporosis patients in the world, and there are more women than men. According to the standards of the World Health Organization (WHO), the results of the US National Health and Nutrition Survey (NHANES III, 1988~1994) showed that osteoporosis seriously affects the quality of life of the elderly. Among the people over 50 years old, 1 / 2 of women, One fifth of men will suffer from osteoporotic fractures in their lifetime. Once a patient experiences the first osteoporotic fracture, the risk of secondary fractures increases significantly. The elderly in China rank first in the world, and there are 90 million osteoporosis patients, accounting for 7.1% of the total population. With the aging of society, the incidence of osteoporosis is on the rise, and it is expected to increase...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N15/62C12N15/63C07K19/00C12R1/19
Inventor 余琼
Owner HEILONGJIANG UNIV