Application of caffeic acid in preparation of medicines for inhibiting leucocyte Src activation

A technology of white blood cells and caffeic acid, which can be used in drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc., and can solve problems such as reducing HHcy patients.

Inactive Publication Date: 2013-04-10
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, folate / Vitamin B6 / Vitamin B12 supplementation is usually used to reduce the blood Hcy level of HHcy patients, but whether this treatment can inhibit the inflammatory response induced by Hcy is still controversial

Method used

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  • Application of caffeic acid in preparation of medicines for inhibiting leucocyte Src activation
  • Application of caffeic acid in preparation of medicines for inhibiting leucocyte Src activation
  • Application of caffeic acid in preparation of medicines for inhibiting leucocyte Src activation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0202] Example 1 Extraction of Caffeic Acid in Salvia Miltiorrhiza

[0203] (a) adjust the pH of the salvia miltiorrhiza decoction to 2.0-3.5 with acid, leave it for 8-24 hours, and filter to obtain the filtrate;

[0204] (b) adding ethanol to the filtrate to a final volume concentration of 75 ± 10%, then standing for 36-72 hours, and filtering to obtain the filtrate;

[0205] (c) Adjust the pH of the filtrate to 7.5-8.5 with alkali, leave it for 8-24 hours, and filter to obtain the filtrate

[0206] (d) adjusting the pH of the filtrate to 5.0-7.0 with an acid, reclaiming ethanol and obtaining a concentrate;

[0207] (e) decolorizing the concentrated solution with activated carbon, and filtering to obtain the filtrate;

[0208] (f) adjust the filtrate to pH 6.8±0.5, and the obtained extract contains caffeic acid.

Embodiment 2

[0209] Example 2 Extraction optimization process of caffeic acid in Salvia miltiorrhiza

[0210] (a) Acidify the salvia miltiorrhiza decoction with hydrochloric acid solution (5%-30%) to pH value (2.0-3.5), let it stand for several hours, and filter to obtain the filtrate.

[0211] (b) Add ethanol to the filtrate until the alcohol content is 75% to 85%, stand in a cold place for tens of hours, and filter to obtain the filtrate.

[0212] (c) The filtrate was adjusted to pH (7.5-8.5) with sodium hydroxide solution (5%-40%), left to stand in a cold place for several hours, and filtered to obtain the filtrate.

[0213] (d) Adjust the pH value to 5.0-7.0 with hydrochloric acid solution (5%-30%), recover ethanol, and obtain a concentrated solution.

[0214] (e) The concentrate is added to several times the amount of water for injection, refrigerated, filtered, and the filtrate is concentrated to obtain a concentrate. The concentrate can be refrigerated. Then add an appropriate am...

Embodiment 3

[0216] Example 3 Extracting caffeic acid with other caffeic acid-containing medicines

[0217] Caffeic acid extracts can be derived from goldenrod or dandelion of Compositae plants, red fruit of rose plants, willow willows of Sapinaceae plants, Cimicifuga rhizomes of Ranunculaceae plants, rhizomes of Eurasian water keel plants of the family Asteraceae, lemon peel of plants of Rutaceae , the whole plant of Polygonaceae, the root of valerian root of Patrinaceae, the whole herb of thyme of Labiatae or the leaves of Eucommia ulmoides, etc. The content of caffeic acid in the above extract is preferably 1-95%. The extraction method is briefly described as follows: taking dandelion as an example, feed dandelion and water at a bath ratio of 1:5-1:40, heat and keep warm for extraction, filter and collect the filtrate, concentrate the filtrate to a certain concentration, and spray dry to obtain powder.

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Abstract

The invention discloses a case that caffeic acid can inhibit homocysteine induced leucocyte non-receptor tyrosine kinase Scr activation, and can further inhibit a PI3K/Akt signal pathway, the leucocyte adhesion molecule integrin beta2 expression, and the hyperhomocysteinemia induced rolling and adherence of leucocytes in leptomeningeal venules of mice, so the caffeic acid can control the hyperhomocysteinemia initiated cerebrovascular disease degeneration diseases.

Description

technical field [0001] The invention relates to a new application of caffeic acid, in particular to the application of caffeic acid in the preparation of a drug for inhibiting the activation of leukocyte Src. Background technique [0002] Caffeic acid (Caffeic acid, hereinafter referred to as CA) can be bought from the market. It exists in many kinds of Chinese medicinal materials, such as salvia miltiorrhiza, coffee compositae, etc., and has significant antioxidant, anti-inflammatory, antibacterial and antiviral properties. , anti-snake venom and other effects, and the toxicity is small, the LD50 of intraperitoneal injection of mice is 1583mg / kg; intravenous injection of 14mg / kg / day in rabbits, for ten consecutive days, no pathological changes were found in the heart, liver, kidney function and anatomical microscope examination . [0003] In vitro studies have shown that CA can inhibit the apoptosis of HUVECs induced by hydrogen peroxide, and can regulate the expression of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/192A61P9/00A61P9/10
Inventor 韩晶岩赵海苹王宪刘育英孙凯卫晓红
Owner TIANJIN TASLY PHARMA CO LTD
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