Nucleic acid amplification and detection reaction tube

Abstract

The invention relates to a nucleic acid amplification and detection reaction tube. The nucleic acid amplification and detection reaction tube comprises a tube body and a tube cover which are mutually matched, wherein the tube body comprises a liquid storage area and a nucleic acid amplification area, the liquid storage area is arranged above the nucleic acid amplification area, and reactions and real-time detection means such as fluorescent signal acquisition and the like are completed in the nucleic acid amplification area; and a hollow groove is arranged in the tube cover, and the volume of the nucleic acid amplification area is respectively less than or equal to the volume of the liquid storage area and the volume of the hollow groove. After the reactions in the nucleic acid amplification area are completed, the reaction tube can be reversed, products in the nucleic acid amplification area can enter the hollow groove of the tube cover in centrifuging, vibration or other modes, and after the tube cover is opened, a small amount of samples is directly taken out to carry out detection in electrophoresis, chromatography or other modes, therefore, an operation step of tube replacement is omitted so as to reduce the probability of pollution.

Description

technical field [0001] The invention relates to an experimental equipment, in particular to a nucleic acid amplification and detection reaction tube. Background technique [0002] Polymerase chain reaction technology (hereinafter referred to as PCR technology) is a technology for rapidly amplifying DNA in vitro. Each cycle includes three processes of denaturation, annealing and extension, and after each cycle, the number of target nucleic acid molecules is doubled. After 30-40 cycles, the number of target nucleic acid molecules was amplified to nearly 10 9 times. PCR is an effective method to obtain a large number of target DNA fragments in vitro, which is convenient for further analysis and inspection of nucleic acid molecules. At present, PCR technology has been widely used in basic research and applied research. As a "cell-free gene amplification system", PCR can be used in basic research to clone genes, and on this basis, perform direct sequence analysis on genomic D...

AI Technical Summary

Problems solved by technology

[0007] (1) It takes a long time. It generally takes 2-3 hours for conventional PCR to complete 30 cycles, and most of the time is consumed in the heating and cooling process, that is, the metal block reaches the equilibrium temperature and the heat is transferred to the PCR reaction solution through the reaction tube;

[0008] (2) The instrument has complex structure, high cost and high energy consumption;

[0009] (3) The program setting is complex and requires trained professionals to operate

However, in the current prior art, there is still no test tube or reactor structure suitable for applying the above-mentioned novel PCR method

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