Method for eliminating antibiotics resistance gene pollution in water by hydrogen peroxide coupled ultrasonic method

A technology of antibiotic resistance and hydrogen peroxide, which is applied in the direction of oxidized water/sewage treatment, chemical instruments and methods, natural water treatment, etc., can solve the problems of antibiotic resistance gene pollution of water, and achieve remarkable treatment effect and penetrating power Strong, long-distance effect

Inactive Publication Date: 2013-06-19
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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Problems solved by technology

[0005] The present invention is to solve the problem of antibiotic resistance gene pollution of water body, and provide a method

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  • Method for eliminating antibiotics resistance gene pollution in water by hydrogen peroxide coupled ultrasonic method

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Embodiment 1

[0015] The method for eliminating antibiotic resistance gene pollution in water bodies based on hydrogen peroxide coupled with ultrasonic radiation is carried out in the following steps: 1. Take 5L of water samples from the secondary settling tank of a sewage treatment plant in Jimei District, Xiamen City, amplify by PCR, and detect The following eight antibiotic resistance genes were identified: integron genes int 1, int 2. Sulfonamide resistance gene sul 1, sul 2, sul A, Tetracycline resistance gene tet A. tet E. tet M; first add coagulant polyaluminium chloride 4mg / L in this water sample, precipitate 30min, make its turbidity lower than 20NTU; Hydrogen oxide solution 50g, at the same time, use ultrasonic cell disintegrator to carry out ultrasonic treatment to it, ultrasonic frequency is 30kHz, ultrasonic sound energy density is 80W / L, process 30min; 3, carry out PCR to the effluent water sample obtained in step 2 Amplify and detect the concentration of antibiot...

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Abstract

The invention discloses a method for eliminating antibiotics resistance gene pollution in water by a hydrogen peroxide coupled ultrasonic method and belongs to the technical field of water treatment. The method provided by the invention is used for solving the problem that the water is polluted by the antibiotics resistance gene. The method is characterized in that the method of feeding hydrogen peroxide into water in combination with ultrasonic radiation is employed so that the antibiotics resistance gene is broken from the point of chemical reaction and basic groups, riboses and phosphoric acids in the antibiotics resistance gene are oxidized by means of strong oxidizing property of the hydrogen peroxide, and consequently, the chemical nature of the antibiotics resistance gene is changed and has no ability of storing and transferring genetic information, on one hand, and on the other hand, many cavitation bubbles are formed in water by means of ultrasonic. When the cavitation bubbles are broken, strong physical destruction effect is generated on the antibiotics resistance gene in water so that the double helix structure of the gene is decomposed and the nucleotide chain of the gen is broken; as a result, the genetic information contained in the nucleotide chain cannot be transferred; therefore, the purpose of eliminating the antibiotics resistance gene pollution is achieved.

Description

technical field [0001] The invention belongs to the field of water treatment, in particular to a method for eliminating antibiotic resistance gene pollution in water bodies. Background technique [0002] In my country, the pollution of antibiotic resistance genes is becoming more and more serious. Antibiotic resistance gene refers to a gene that can make microorganisms resistant to antibiotic substances. In a microbial population, not all individuals have antibiotic resistance genes, and since such genes are often produced by mutation, the gene frequency is low. However, in an environment containing a large amount of antibiotics, the probability of microorganisms producing antibiotic resistance genes is greatly increased. Research confirms that microbes can release their own antibiotic resistance genes into the environment. Antibiotic resistance genes that exist in the environment can enter the microorganisms that are not originally resistant to antibiotics through variou...

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Application Information

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IPC IPC(8): C02F1/72C02F1/30C02F1/58C02F103/38
Inventor 赵峰王鲁刘玉蕾
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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