Molecular specificity marker primer and method for distinguishing Chinese bolete and European bolete

A technology for labeling primers and boletus, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of subjective discrimination bias, difficult color difference, etc., and achieve the effect of simple method
CN103173541AInactive Publication Date: 2013-06-26ZHEJIANG FORESTRY ACAD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG FORESTRY ACAD
Publication Date
2013-06-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a molecular specificity marker primer for distinguishing Chinese bolete B. roseoflavus and European bolete B. appendiculatus, and a method for quickly distinguishing Chinese bolete B. roseoflavus and European bolete B. appendiculatus by utilizing the primer. The nucleotide sequence of the molecular specificity marker primer is as follows: the upstream primer is 5'-CTTGCTCAGCCCAACTCAACG-3'; and the downstream primer is 5'-ATCTCTGGGTGGAGCATGTGCACG-3'. The molecular specificity marker primer and the method have the beneficial effects that quick molecular identification can be carried out on sibling species Chinese bolete B. roseoflavus and European bolete B. appendiculatus through the molecular specificity marker primer, and the method is simple, quick and accurate and is an effective assistant for distinguishing two types of bolete through morphology.
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Description

(1) Technical field

[0001] The invention relates to a molecular specific marker primer for distinguishing Chinese Boletus roseoflavus and European Boletus B. appendiculatus, and a method for quickly distinguishing Chinese and European Boletus specimens by using the primer. (2) Background technology

[0002] Both Boletus roseoflavus and B.appendiculatus belong to the Appendiculati Konrad & Maubl.ex Lannoy & Estadès group of the Boletus genus under the Boletus order Boletales. So far, only B. roseoflavus has been found in China, and B. appendiculatus in Europe. The identification features of B.roseoflavus in China are light pink, purple, rose red cap, the flesh does not turn blue when exposed to the air, or slowly turns blue, the pore turns blue immediately after injury, and the average aspect ratio of basidiospores less than 3. The identification features of B. appendiculatus in Europe are light to dark brown cap without pink color; the flesh turns blue obviously when expos...

Claims

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