In vitro tissue cultivation method of potted anthurium andraeanum varieties

A technology of tissue culture and variety, applied in the field of in vitro tissue culture of anthurium potted plant explants, can solve the problems of difficult to meet the needs of the flower market, different conclusions, no relatively unified treatment methods, etc., and achieve good application The effect of high value and differentiation rate

Inactive Publication Date: 2013-07-03
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Anthurium has a long growth cycle, and conventional seed propagation and branch propagation are difficult to meet the needs of the flower market. Plant tissue culture technology is undoubtedly an effective way to shorten the propagation cycle and supply a large number of seedlings, and can ensure the excellent variety characteristics of the female parent.
Since the 1970s, foreign Pierk and others successfully established anthurium tissue culture system by using leaf explants, the tissue culture propagation method has gradually replaced the traditional propagation method. characteristi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The leaves and petioles of unexpanded leaves of Anthurium potted cultivars for 5 days were selected as explants, and the surface of the materials was washed with lotion, rinsed under running water for 30-50 min, taken out and dried in the air; the washed leaves and petioles were , with a mass concentration of 0.05% to 0.1% HgCl 2 Treat for 3-10 minutes, rinse with sterile water 5-6 times. In the ultra-clean workbench, the leaf explants were inoculated in medium 1 / 2 MS+ TDZ 2.0 mg / L+ 2,4-D 0.2 mg / L+3% sucrose, and the petiole explants were inoculated in medium 1 / 2 MS+ TDZ 4.0 mg / L+ 2,4-D 0.2 mg / L+3% sucrose; light 12 h / d, light intensity 2000 lx. After 30 days of culture, both ends of the petiole incision swelled, and yellow-green callus was produced, with a callus rate of 41.67%. After 60 days of continuous culture, the average number of adventitious buds per callus was 5; Callus, yellow-green callus was produced on the edge of the incision, and the callus rate reache...

Embodiment 2

[0019] The leaves and petioles of unexpanded leaves of Anthurium potted cultivars for 5 days were selected as explants, and the surface of the materials was washed with lotion, rinsed under running water for 30-50 min, taken out and dried in the air; the washed leaves and petioles were , with a mass concentration of 0.05% to 0.1% HgCl 2 Treat for 3-10 minutes, rinse with sterile water 5-6 times. In the ultra-clean workbench, inoculate the leaf and petiole explants in the medium 1 / 2MS+ TDZ 4.0 mg / L+ 2,4-D 0.2 mg / L+3% sucrose, light 12 h / d, light intensity 2000 lx . After 30 days of culture, both ends of the petiole incision swelled, and yellow-green callus was produced. The callus rate was 87.5%. After 60 days of culture, the average number of adventitious buds per callus was 12. The leaves produced callus after 50 days of culture, and yellow-green callus appeared on the edge of the incision, and the callus rate reached 80%. After 60 days of continuous culture, the average nu...

Embodiment 3

[0021] The leaves and petioles of unexpanded leaves of Anthurium potted cultivars for 8 days were selected as explants, and the surface of the materials was washed with lotion, rinsed under running water for 30-50 min, taken out and dried in the air; the washed leaves and petioles were , with a mass concentration of 0.05% to 0.1% HgCl 2 Treat for 3-10 minutes, rinse with sterile water 5-6 times. Leaf and petiole explants were inoculated in medium 1 / 2 MS+ ZT 2.0 mg / L+ 2,4-D 0.2 mg / L+3% sucrose, light 12 h / d, light intensity 2000 lx. After 30 days of culture, both ends of the incision of the petiole swelled, producing yellow-green callus, and the healing rate was 45.83%. %. After continuing to culture for 60 days, the leaf explants differentiated into adventitious buds again, and the average number of adventitious buds differentiated per piece of callus was 2.6, and no adventitious buds differentiated from petiole. In vitro plantlet subculture medium 1 / 2 MS + 6-BA 1.0 mg / L + ...

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Abstract

The invention discloses an in vitro tissue cultivation method of potted anthurium andraeanum varieties. A leaf blade, a leaf stem, a flower head, a spathe blade or a lateral bud is taken as an explant material; after the explant material is sterilized, the explant material is inoculated to an appropriate culture medium and cultivated for 40-60 days, and then dedifferentiates to form a callus, and the callus rate can reach 87.5%; the material is continuously cultivated for 40-60 days and differentiates again to form an adventive bud, and the differentiation rate can reach 94.12%; and a large quantity of tube seedlings can be rapidly cultivated through multiplication, when the tube seedlings grow to certain heights, the tube seedlings are transferred into the culture medium and transplanted after the tube seedlings take root, and the survival rate can be larger than 95%. With the adoption of an in vitro tissue cultivation technology, a regeneration plant can be stably and efficiently obtained through the way that the callus induces the adventive bud, and rapid and mass propagation of the potted anthurium andraeanum varieties can be achieved; and according to the characteristics of different anthurium andraeanum varieties, the appropriate explant material and a culture medium condition are selected, and the method has a high application value in the production of seedlings of excellent anthurium andraeanum varieties.

Description

technical field [0001] The invention relates to a method for in vitro tissue culture of anthurium potted plant species explants. Background technique [0002] Anthurium ( Anthurium andraeanum ) is a perennial herbaceous flower of the genus Anthurium in the Araceae family. It is native to the tropical rain forests of South America and likes high temperature and high humidity environmental conditions. Anthurium has shortened stems, aerial roots on nodes, plant height 50-80cm, leaves gathered on top of stems, leathery, heart-shaped, oval, etc. Flowers and leaves grow in turn, a branch of flower emerges from one leaf, spathe is heart-shaped and oval, and the colors are red, pink, white, green, etc. The spadix is ​​sessile and yellow, and can bloom all year round under suitable growth conditions . Because of its unique flowers and leaves, the flowering period lasts for several months, so it is widely used in indoor and outdoor flower decoration. [0003] Anthurium has a long...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 谈建中周丽丽张树初郑必平王晶闫俊芳牛瑞鹤陈驰
Owner SUZHOU UNIV
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