Method for producing melanin by eurotium cristatum utilizing levoglucosan as well as cellulose pyrolysis solution and so on

A technology of inner ether sugar and saccharomyces, which is applied in the field of melanin production, and can solve the problems of limited sources, toxicity of chemical synthesis and products with complicated steps

Active Publication Date: 2013-07-17
巴州正达绿源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, melanin is mostly obtained through chemical synthesis or extraction from animals and plants. The toxicity and complicated steps of chemical synthesis limit the application of products, and the extraction from animals and plants has a problem of limited sources.

Method used

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  • Method for producing melanin by eurotium cristatum utilizing levoglucosan as well as cellulose pyrolysis solution and so on
  • Method for producing melanin by eurotium cristatum utilizing levoglucosan as well as cellulose pyrolysis solution and so on
  • Method for producing melanin by eurotium cristatum utilizing levoglucosan as well as cellulose pyrolysis solution and so on

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Prepare 2% inner ether sugar (W / W) liquid medium, other ingredients in the formula are 1% peptone, 0.1% KH 2 PO 4 , 0.04%MgSO 4 , 0.01%FeSO 4 , pH5.0. Inject 10% (W / W) Saccharomyces coronis (isolated from commercially available black tea by the inventor) bacterial liquid, and culture it dynamically at 30°C and 180rpm. HPLC analysis results see figure 1 with figure 2 shown by figure 1 with figure 2 visible, figure 1 The 5.6min in the middle is the inner ether sugar, and after being cultured for 7 days after being connected with S. coronis, figure 2 The inner ether sugar completely disappeared, and the utilization rate of 2% inner ether sugar was 100%.

Embodiment 2

[0033] In embodiment 1, 2% inner ether sugar is changed into 4% cellulose pyrolysis solution, other conditions are the same, the HPLC analysis of pyrolysis solution as it is and conversion solution sees figure 2 with image 3 shown by image 3 with Figure 4 visible, image 3 5.7min in the middle is the inner ether sugar, and after being cultured for 7 days after being connected with the coronoid, the utilization rate of the inner ether sugar in the 4% pyrolysis solution is 91%

Embodiment 3

[0035] Prepare 2% inner ether sugar (W / W) liquid medium, the other ingredients in the formula are 1% NH 4 NO 3 , 0.2%K 2 HPO 4 , 0.3%MgSO 4 , pH 4.0. Insert the Saccharomyces coronaris bacteria liquid, and culture it dynamically for 7 days at 30°C and 180rpm, take 20ml of fermentation broth, add an equal volume of 1mol / L NaOH to extract melanin by conventional methods, and dry to obtain crude melanin 1.1 g / L.

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Abstract

The invention relates to the utilization of eurotium cristatum onto melanin produced by carbon sources including a cellulose biomass high-temperature pyrolysis product pyrolysis solution, levoglucosan and the like. The eurotium cristatum is grown by using levoglucosan, maltose, glucose, sucrose, lactose, molasses, stevia rebaudiana, pyrolysis solution and the like as carbon sources, and can be used for producing melanin.

Description

technical field [0001] The invention relates to a method for producing melanin by using the fungi and different carbon sources. Specifically, it involves the production of melanin by Eurotium using carbon sources such as endose, maltose, glucose, sucrose, lactose, molasses, stevia and pyrolyzed liquid. Background technique [0002] Cellulosic biomass is the most abundant renewable resource on earth. Lactose, molasses, stevia, etc. are also by-products or raw materials produced in the dairy industry, sugar cane, sugar beet, and stevia sugar industry. The effective use of these renewable resources It has important practical significance for alleviating resource problems. [0003] Cellulosic biomass includes trees, bamboo, crop straw, etc. (Mckendry P. Energy production from biomass (part1): overview of biomass [J]. Bioresource Technology, 2002, 83: 37-46). Under certain pyrolysis conditions (high temperature, anoxic), biomass can generate pyrolysis products mainly including ...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/645
Inventor 陈育如孙欢钱媛媛刘军利卫民
Owner 巴州正达绿源生物科技有限公司
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