Cartilage graft for cartilage injury repair and preparation method thereof

Abstract

The invention discloses a cartilage graft for cartilage injury repair and a preparation method thereof. The cartilage graft is prepared based on an adipose-derived stem cell-cartilage cell co-culture technology. The preparation method is effective and safe, can prepare enough cartilage cells for transplant in a short time, can efficiently transform cartilage tissue and can improve cartilage tissue transplant treatment effects. The preparation method utilizes nutrition functions of adipose-derived stem cells and carries out co-culture of adipose-derived stem cells and cartilage cells in a three-dimensional culture system. Through interaction of the adipose-derived stem cells and the cartilage cells, proliferation and activity of the cartilage cells are promoted. The adipose-derived stem cells are influenced by the environment and are partly differentiated into cartilage cells and thus under the condition of undersampling or activity lack of the cartilage cells, a high-quality cartilage graft can be obtained and graft viability is promoted and shallow-layer cartilage coloboma can be repaired well.

Description

technical field [0001] The invention relates to a cartilage graft, in particular to a cartilage tissue graft formed by co-cultivating adipose stem cells and chondrocytes in a three-dimensional collagen medium for cartilage damage and a preparation method thereof. Background technique [0002] Cartilage damage is a difficult disease to heal, especially superficial cartilage damage. Cartilage damage can be caused by a variety of reasons, such as external impact, autoimmune diseases, body degeneration caused by aging, etc. The self-repair ability of cartilage is very weak, and even a small cartilage defect is difficult to heal. Because there is no blood supply within the cartilage tissue, endochondral matrix synthesis is not very active. However, if the wound is deep enough to the subchondral bone layer, the wound may heal itself. This is because the blood in the subchondral bone seeps out to form a blood clot, and the nearby stem cells may be activated to promote wound heali...

AI Technical Summary

Problems solved by technology

The main problem is that this culture system only contains a single chondrocyte component, while in the body, the cells are in a three-dimensional space, the coexistence of multiple cells, the interaction between cells, signal transduction, etc. have a great impact on the function of cells

A culture system with a single component will cause the cells to grow slowly, be inactive and dedifferentiated, and after implantation in the body, there will be no continuous nutrient supply, which will lead to a decrease in cell survival rate

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