Cartilage graft for cartilage injury repair and preparation method thereof

A technology for cartilage damage and grafts, applied in the field of cartilage grafts, can solve the problems of great influence on cell function, lower cell survival rate, slow cell growth, etc., achieve short culture time, improve proliferation ability and activity, improve activity and The effect of the function

Inactive Publication Date: 2013-07-31
GUANGZHOU LAND BIOLOGY TECH
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem is that this culture system only contains a single chondrocyte component, while in the body, the cells are in a three-dimensional space, the coexistence of multiple cells, the interaction between cells, signal transduction, etc. have a great impact on the function of cells
A culture system with a single component will cause the cells to grow slowly, be inactive and dedifferentiated, and after implantation in the body, there will be no continuous nutrient supply, which will lead to a decrease in cell survival rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cartilage graft for cartilage injury repair and preparation method thereof
  • Cartilage graft for cartilage injury repair and preparation method thereof
  • Cartilage graft for cartilage injury repair and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] In order to describe the technical content of the present invention, the objectives and effects achieved, the following will be described in detail in conjunction with the embodiments and accompanying drawings. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the authorization of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0026]All reagents used for cell culture were purchased from Gibco, Invitrogen, unless otherwise stated. Common chemicals were purchased from Sigma-Aldrich.

[0027] 1. Preparation of ADSCs

[0028] 1) About 10 days before cartilage sampling, about 20ml of fat was extracted from the patient's thigh or abdomen, a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a cartilage graft for cartilage injury repair and a preparation method thereof. The cartilage graft is prepared based on an adipose-derived stem cell-cartilage cell co-culture technology. The preparation method is effective and safe, can prepare enough cartilage cells for transplant in a short time, can efficiently transform cartilage tissue and can improve cartilage tissue transplant treatment effects. The preparation method utilizes nutrition functions of adipose-derived stem cells and carries out co-culture of adipose-derived stem cells and cartilage cells in a three-dimensional culture system. Through interaction of the adipose-derived stem cells and the cartilage cells, proliferation and activity of the cartilage cells are promoted. The adipose-derived stem cells are influenced by the environment and are partly differentiated into cartilage cells and thus under the condition of undersampling or activity lack of the cartilage cells, a high-quality cartilage graft can be obtained and graft viability is promoted and shallow-layer cartilage coloboma can be repaired well.

Description

technical field [0001] The invention relates to a cartilage graft, in particular to a cartilage tissue graft formed by co-cultivating adipose stem cells and chondrocytes in a three-dimensional collagen medium for cartilage damage and a preparation method thereof. Background technique [0002] Cartilage damage is a difficult disease to heal, especially superficial cartilage damage. Cartilage damage can be caused by a variety of reasons, such as external impact, autoimmune diseases, body degeneration caused by aging, etc. The self-repair ability of cartilage is very weak, and even a small cartilage defect is difficult to heal. Because there is no blood supply within the cartilage tissue, endochondral matrix synthesis is not very active. However, if the wound is deep enough to the subchondral bone layer, the wound may heal itself. This is because the blood in the subchondral bone seeps out to form a blood clot, and the nearby stem cells may be activated to promote wound heali...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38
Inventor 付擎昊康贤通章戴荣
Owner GUANGZHOU LAND BIOLOGY TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap