Nile tilapia NPB29 (neuropeptide B29) peptide as well as encoding nucleic acid and application thereof

A technology of Nile tilapia and tilapia, which is applied in the field of functional application of 29 peptide, can solve the problems that the functional research is still blank

Active Publication Date: 2013-09-04
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In mammals, NPB mainly exists in the form of 29-peptide, widely distributed in the central nervous system, and participates in various physiological functions such as food intake regulation, energy metabolism balance, endocrine regulation and pain perception, but the functional research of NPB in teleosts is still unknown. blank

Method used

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  • Nile tilapia NPB29 (neuropeptide B29) peptide as well as encoding nucleic acid and application thereof
  • Nile tilapia NPB29 (neuropeptide B29) peptide as well as encoding nucleic acid and application thereof
  • Nile tilapia NPB29 (neuropeptide B29) peptide as well as encoding nucleic acid and application thereof

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Embodiment Construction

[0025] Below in conjunction with experimental data, further illustrate the present invention.

[0026] Cloning of NPB cDNA from Nile Tilapia

[0027] (1) Primer design

[0028] Using the EST retrieval function of NCBI, we found a part of the predicted sequence XM_003450122.1 published in tilapia, and then designed specific primers to clone partial fragments of NPB cDNA, and then designed 5`-RACE and Specific primers for 3'RACE, so as to carry out 5'-RACE and 3'RACE with the designed degenerate primers. . Finally, specific primers were designed in the 3' and 5' non-coding regions to clone the open reading frame ORF.

[0029] (2) Cloning of the middle fragment of Nile tilapia NPB cDNA

[0030] The first strand cDNA was synthesized from the total RNA using the Rever Tra Ace-a-Tm First Strand cDNA Synthesis Kit from Toyobo. The PCR amplification of the middle fragment was carried out according to the Toyobo product polymerase blend tap plus. The first round of PCR reaction c...

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Abstract

The invention discloses a nile tilapia NPB29 (neuropeptide B29) peptide as well as an encoding nucleic acid and an application thereof. The sequence of the NPB29 peptide is shown in SEQ ID NO:1. Experiments prove that after the NPB29 peptide disclosed by the invention is injected to the tilapia by means of intraperitoneal injection, the expression of feeding factors of NPY (neuropeptide Y) and CCK1 (cholecystokinin1) in the brain of the tilapia can be stimulated, the GH (Growth Hormone) in hypophysis can be inhibited, and PRL (prolactin) can be stimulated, thereby proving that the NPB functional 29 peptide plays an important role in feeding and growth control.

Description

technical field [0001] The invention relates to a new protein of Nile tilapia and its encoding nucleic acid, and at the same time, the invention also relates to the functional application of the 29 peptide of the new protein. Background technique [0002] Tilapia is a small to medium sized fish. Now it is a freshwater farmed fish cultivated by key scientific research in the world's aquaculture industry, and it is known as one of the main sources of animal protein in the future. Native to Africa, it is similar to sea bass. Usually live in fresh water, can also live in salt water with different salinity content, can survive in shallow water in lakes, rivers, and ponds. It has strong adaptability, and has strong adaptability to water with less dissolved oxygen. Most tilapia are omnivorous, often eating plants and debris in the water. As a substitute for cod, tilapia is increasingly in demand in the international market. In the global freshwater fish trade, tilapia occupies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C12N15/12C12N15/10A23K1/18A23K1/16
Inventor 李文笙杨露李波
Owner SUN YAT SEN UNIV
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