Application of Sarcaboside B in preparation of medicines for treating lung cancer

A drug and lung cancer technology, applied in the field of anti-tumor drugs, can solve the problem of damage to normal tissues and cells, and achieve the effect of strong cytotoxicity and prominent substantive characteristics

Inactive Publication Date: 2013-10-23
丁圣雨
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Malignant tumors are diseases that seriously threaten human life and health. Chemotherapy drugs commonly used in clinical practice can greatly damage normal tissues and cells while killing tumor cells, which limits its application.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Sarcaboside B in preparation of medicines for treating lung cancer
  • Application of Sarcaboside B in preparation of medicines for treating lung cancer
  • Application of Sarcaboside B in preparation of medicines for treating lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Preparation of the compound Sarcaboside B tablet of the present invention:

[0014] Take 20 grams of compound Sarcaboside B, add 180 grams of conventional excipients for preparing tablets, mix well, and use a conventional tablet machine to form 1000 tablets.

Embodiment 2

[0015] Example 2: Preparation of the compound Sarcaboside B capsules involved in the present invention:

[0016] Take 20 grams of compound Sarcaboside B, add 180 grams of conventional auxiliary materials for preparing capsules such as starch, mix well, and encapsulate to make 1000 tablets.

[0017] The pharmacodynamics experiment will further illustrate its drug activity below.

experiment example

[0018] Experimental example: Sarcaboside B inhibits the proliferation of lung cancer cells cultured in vitro and affects human normal cells

[0019] Lung cancer cells in logarithmic growth phase (non-small cell lung cancer cell A 549 , large cell lung cancer cells NCI-H460, lung squamous cell carcinoma cells HTB-8 and human lung squamous cell carcinoma cells QG-5) and human normal hepatocytes L02 were inoculated on 96-well culture plates, and the inoculation volume per well was 3000-4000 Cells / 100uL and 6000 cells / 100uL; aseptically collected 15 mL of venous blood from healthy blood donors, anticoagulated with heparin, separated mononuclear cells by Ficoll density gradient centrifugation, and prepared a single cell suspension with RPMI1640 containing 10% fetal bovine serum. Adjust the cell concentration to 2 × 10 5 100 uL per well, inoculated in 96-well culture plates. After culturing the cells in the above plates for 24 hours, 100 uL of different concentrations of Sarcabosi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an application of Sarcaboside B in preparation of medicines for treating lung cancer. The Sarcaboside B has good anti-lung cancer activity and can be used for the preparation of anti-lung cancer medicines, belonging to the technical field of new applications of medicines. The Sarcaboside B can be used for obviously inhibiting non-small cell lung cancer cells A549 cultured in vitro, giant cell lung cancer cells NCI-H460, lung squamous carcinoma cells HTB-8 and human lung epidermoid carcinoma cells QG-5 and has a little inhibiting effect on human normal hepatic cells LO2 and peripheral blood mononuclear cells and obvious selectivity. The application of the Sarcaboside B in preparation of the medicines for treating lung cancer is disclosed by the invention for the first time. As the skeleton type of the Sarcaboside B belongs to a brand-new skeleton type, the Sarcaboside B has unexpected strong inhibitory activity on lung cancer cells.

Description

technical field [0001] The invention relates to the field of antitumor drugs, in particular to the application of Sarcaboside B in the preparation of antitumor drugs. Background technique [0002] Malignant tumor is a disease that seriously threatens human life and health. At present, the commonly used chemotherapeutic drugs not only kill tumor cells, but also greatly damage normal tissues and cells, which limits its application. It has become the direction and urgent task of current anti-cancer drug research to find high-efficiency and low-toxic drugs for the treatment of tumors. Searching for high-efficiency, low-toxicity antitumor drugs has been considered an effective way. [0003] The present invention confirms through experiments that Sarcaboside B has effect on non-small cell lung cancer cells A cultured in vitro 549 , large cell lung cancer cells NCI-H460, lung squamous cell carcinoma cells HTB-8 and human lung squamous epithelial cancer cells QG-5 have significant...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P35/00
Inventor 丁圣雨
Owner 丁圣雨
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap