Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting bladder cancer-related risk genes

A kit, technology for bladder cancer, applied in the fields of molecular biology and medicine

Inactive Publication Date: 2013-10-23
浙江爱易生物医学科技有限公司
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there was no significant difference in the number of apoptotic cells between all C-myc positive cases and all negative cases (P>0.05). The number of apoptotic cells did not increase with the increase of C-myc positive rate, but with the malignant transformation of bladder cancer. C-myc expression was negatively correlated with apoptosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Use of detection kits

[0028] Step 1: Extraction of DNA template

[0029] Vacuum blood collection tubes are used to collect human peripheral blood, and the genomic DNA in the blood is extracted.

[0030] Step 2: Real-time quantitative PCR reaction

[0031] Use a fluorescent quantitative PCR detection kit that can detect bladder cancer-related risk genes, which includes the following primer pairs and fluorescent probe pairs:

[0032] Sense Primer 1: 5'- GCAAAGGCTGGAGTT - 3' (SEQ ID NO: 1)

[0033] Antisense Primer 1: 5'- TAATAATTAACAAAG - 3' (SEQ ID NO: 2)

[0034] Sense Primer 2: 5'- CTCCACCACAGCCCA - 3' (SEQ ID NO: 3)

[0035] Antisense Primer 2: 5'-CAACAGGGCAAGCAG-3' (SEQ ID NO: 4)

[0036] Probe 1 with VIC fluorophore: 5'-GAGAACCCgTGGTTGA-3' (SEQ ID NO: 5)

[0037] Probe 1 with FAM fluorophore: 5'-GAGAACCCtTGGTTGA-3' (SEQ ID NO: 6)

[0038]Probe 2 with VIC fluorophore: 5'- AGTGACCAcGAAGGCT -3' (SEQ ID NO: 7)

[0039] Probe 2 with FAM fluorophore: ...

Embodiment 2

[0047] Example 2. Services for bladder cancer-related risk gene testing for patients

[0048] Step 1: DNA Extraction

[0049] The laboratory physicians of the hospital collect peripheral blood from the subjects, use vacuum blood collection tubes to collect peripheral blood, and extract genomic DNA from it.

[0050] Step 2: Genotyping Assays

[0051] Use the kit provided by the present invention to carry out fluorescence quantitative PCR detection on the SNP site No. rs9642880 on the MYC gene of the subject's genomic DNA and the SNP site No. rs2294008 on the PSCA gene respectively, and determine the genes of these two SNPs sites type.

[0052] Step 3: Analysis of Individual Bladder Cancer Risk

[0053] Through the analysis of the SNPs genotypes of the tested subjects, an analysis report of the risk of bladder cancer is issued. The report specifies the risk of the subject suffering from bladder cancer in detail, and the doctor explains and interprets the analysis report shee...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for detecting bladder cancer-related risk genes. The kit includes a specific primer pair and a specific fluorescent probe pair for simultaneously detecting a No. rs9642880 SNP locus on an MYC gene and a No. rs2294008 SNP locus on a PSCA gene, a conventional component for fluorescent quantitative polymerase chain reaction (PCR) detection and the like. The kit can assess risk that an individual suffers from bladder cancer through simultaneously detecting MYC and PSCA single nucleotide polymorphic locus genotypes which are closely related to psoriasis.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine, and more specifically, the present invention relates to a kit for detecting risk genes associated with bladder cancer, by simultaneously detecting single nucleotide polymorphisms of genes MYC and PSCA associated with bladder cancer Point genotypes were used to assess an individual's risk of developing bladder cancer. Background technique [0002] In my country, the incidence of bladder tumor ranks first in the malignant tumors of the genitourinary system. At present, the clinical treatment of bladder tumors is mainly based on surgery, supplemented by chemotherapy, radiotherapy and immunotherapy. However, it has a high recurrence rate after surgery. Chemotherapy drugs often can only delay the recurrence of tumors, have little effect on the evolution of tumors, and are prone to drug resistance and certain toxicity and side effects. Therefore, people are paying more and mor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 任峻张华忠
Owner 浙江爱易生物医学科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com