Joint detection test paper for ferroprotein and bone alkaline phosphatase in human blood and preparation method thereof

A bone alkaline phosphatase and combined detection technology, applied in the detection of peptides or amino acids, and the field of protein, can solve the problems of inability to obtain quantitative results, not easy for children to accept, and cumbersome to operate, meet the requirements of clinical diagnosis, simplify Test the operation process, the effect of simplifying the operation procedure

Inactive Publication Date: 2013-11-27
天津朗赛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first two methods need to separate blood serum (plasma) from veins for detection, which is not easily accepted by children, and the detection process is complicated and takes a long time; the latter method has a large error in the detection results and cannot obtain quantitative results. And there is also the weakness of cumbersome operation

Method used

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  • Joint detection test paper for ferroprotein and bone alkaline phosphatase in human blood and preparation method thereof

Examples

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Effect test

Embodiment 1

[0032] A test paper for the combined detection of ferritin and bone alkaline phosphatase in human blood, comprising a base plate 1, a nitrocellulose membrane 4 pasted on the middle of the base plate 1, marker pads 3 respectively lapped on both sides of the nitrocellulose membrane 4, an absorbent The sample pad 5 and the sample pad 2 overlapped with the marker pad 3, the first detection strip 6 composed of a ferritin monoclonal antibody, bone alkaline phosphatase The second detection strip 7 made of polyclonal antibody and the quality control strip 8 made of goat anti-mouse IgG antibody, the marker pad 3 is composed of human ferritin monoclonal antibody and colloidal gold-labeled human ferritin on the non-woven fabric. Gold-labeled human bone alkaline phosphatase monoclonal antibody.

[0033] One end of the marker pad 3 is overlapped with the side of the nitrocellulose membrane 4 close to the first detection strip 6 , and the other end is overlapped with the sample loading pad ...

Embodiment 2

[0051] Example 2 Preparation method of test paper for combined detection of ferritin and bone alkaline phosphatase in human blood

[0052] A preparation method of a combined detection test paper for ferritin and bone alkaline phosphatase in human blood, comprising the steps of:

[0053] ① Preparation of marker pad 3:

[0054] a. Prepare a colloidal gold solution with a diameter of 50nm by chloroauric acid-trisodium citrate reduction method;

[0055] b. Take 100ml of the above colloidal gold solution, and use 0.2M K 2 CO 3 The solution adjusts the colloidal gold solution to pH=9.0;

[0056] c. Place the above colloidal gold solution with pH=9.0 on a magnetic stirrer and stir slowly, add 1.5mg ferritin monoclonal antibody and bone alkaline phosphatase antibody for each 100ml solution, and slowly drop them into the colloidal gold solution;

[0057] d. After stirring continuously for 1 hour, add 20% BSA bovine serum albumin to a final concentration of 0.2% and 20% PEG polyethy...

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Abstract

The invention discloses joint detection test paper for ferroprotein and bone alkaline phosphatase in human blood and a preparation method thereof, and belongs to the field of detection of protein, peptide or amino acid. The joint detection test paper comprises a base plate, a cellulose nitrate film adhered to the middle part of the base plate, and a marker cushion and a sample absorbing cushion respectively in lap joint with two sides of the cellulose nitrate film, as well as a sampling cushion in the lap joint with a marker, wherein a first detection strip composed of ferroprotein monoclonal antibodies, a second detection strip composed of bone alkaline phosphatase polyclonal antibodies, and a quality control strip composed of goat-anti-mouse antibodies are wrapped on the cellulose nitrate film in sequence; the marker cushion is composed of human ferroprotein monoclonal antibodies containing colloidal gold markers, and human bone alkaline phosphatase monoclonal antibodies containing colloidal gold markers, and the human ferroprotein monoclonal antibodies and the human bone alkaline phosphatase monoclonal antibodies are arranged on a piece of non-woven fabrics. The joint detection test paper can detect the ferroprotein and the bone alkaline phosphatase in the human blood at the same time, and simplifies the operation process while ensuring that the detection sensitivity and the specific performance can meet the requirement of clinical diagnosis.

Description

technical field [0001] The invention relates to the detection of protein, peptide or amino acid, in particular to a combined detection test paper for ferritin and bone alkaline phosphatase in human blood and a preparation method thereof. Background technique [0002] Ferritin is a protein that stores iron in the body, and its serum (plasma) concentration represents the body's iron reserves. Serum (plasma) ferritin concentrations can also be abnormally elevated as an acute-phase reactive protein during infection or certain tumors. In general, the serum ferritin concentration is between 20 and 200 ug / L, that is, 20-200 ng / ml. [0003] At present, the main detection methods for serum (plasma) ferritin content include radioimmunoassay, enzyme-linked immunoassay, and chemiluminescence. The above-mentioned detection methods all need to rely on corresponding complex instruments and equipment, and also need to use liquid reagents. Therefore, they have defects such as complicated d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/532
Inventor 叶涛
Owner 天津朗赛生物科技有限公司
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