Pseudomonas fluorescens Y13, and preparation method and applications thereof

A Pseudomonas fluorescens, deposit number technology, applied in the direction of biochemical equipment and methods, applications, microbial-based methods, etc., can solve the problems of inflow of clean water and surface water, water pollution, etc., to reduce planting costs and agriculture Input, easy access, and the effect of mitigating environmental pollution

Inactive Publication Date: 2015-04-08
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, in order to increase agricultural production, a large amount of phosphorus fertilizer is applied to the soil, of which only a small part is absorbed by crops, and a large amount of phosphorus fertilizer is converted into a metal chelate state in the soil; part of it even flows into clean water under rainwater washing and surface water bodies, causing water pollution

Method used

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  • Pseudomonas fluorescens Y13, and preparation method and applications thereof
  • Pseudomonas fluorescens Y13, and preparation method and applications thereof
  • Pseudomonas fluorescens Y13, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: a kind of preparation method of Pseudomonas fluorescens Y13 and the detection of phosphate-dissolving ability

[0047] 1. Strain isolation:

[0048] A. Weigh 5g of soil sample into 90mL of sterile saline, shake at 180r / min on a constant temperature shaker at 28°C for 30min, and let stand for 1min.

[0049] B, get supernatant 5mL in Montina organophosphate liquid culture medium (glucose 10.00g, (NH 4 ) 2 SO 4 0.50g, MgSO 4 ·7H 2 O 0.30g, NaCl 0.30g, KCl 0.30g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, CaCO 3 5.00g, lecithin 2.00g, distilled water 1000ML, PH7.0-7.2).

[0050] C. Culture on a constant temperature shaker at 28°C at 180r / min for 3 days.

[0051] D. Inoculate into fresh organophosphorus liquid medium at a ratio of 1:100, continue to cultivate for 3 days, take 1 mL of bacterial liquid for 10-fold serial dilution.

[0052] E. Dilute 100 μL of different dilutions (10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 ) were coated on organic phos...

Embodiment 2

[0068] A kind of application of Pseudomonas fluorescens Y13 in dissolving insoluble soil phosphorus, increasing available phosphorus content, improving plant phosphorus nutrition, its application process is:

[0069] 1. Preparation of microbial inoculum:

[0070] 1) Inoculate the Y13 strain stored on the LB slant to a fresh LB slant and activate overnight at 28°C;

[0071] 2) Inoculate into LB liquid medium, culture overnight at 28°C and 180r / min.

[0072] 3) Then inoculate it into a fresh medium at a ratio of 1:100, and continue to cultivate until the cell concentration reaches 10 7 —10 8 up to cfu / mL.

[0073] 4) Take 100g of peat into the Erlenmeyer flask, sterilize at 120°C for 30min at high temperature.

[0074] 5) Add 10mL of bacterial solution to 100g of sterilized peat, and mix evenly. The water content of the fungicide reaches the point where it will form a ball when you hold it by hand, and it will disperse when you touch it.

[0075] 6) Place the mixed bacteri...

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Abstract

The invention discloses a Pseudomonas fluorescens Y13, and a preparation method and applications thereof. The preparation method comprises the following steps: 1, weighing a soil sample, putting in disinfected normal saline, oscillating on a constant temperature shaking table, and allowing an obtained solution to stand; 2, taking an obtained supernatant to a Mengjinna organophosphorus liquid medium; 3, culturing on the constant temperature shaking table, inoculating to a fresh organophosphorus liquid medium, and continuously culturing; 4, coating an organophosphorus solid flat with different dilution ratios of a dilution, inverting in a constant temperature culture box, and culturing; and 5, purifying single colonies obtained on the flat, and preserving a finally obtained pure culture on an LB inclined plane. An above obtained strain is identified as Pseudomonas fluorescens Y13, is preserved and has a preservation number of CCTCC NO:M2012121. The Pseudomonas fluorescens Y13 has the advantages of easy preparation obtaining, low cost, increase of the content of soluble phosphorus in soil, substantial promotion of the phosphorus element absorption and utilization of plants, high yield of crops, reduction of the planting cost and the agricultural investment, and alleviation of the environmental pollution caused by the excess application of an agricultural phosphorus fertilizer.

Description

technical field [0001] The invention belongs to the field of soil environment restoration and management. More specifically, it relates to a kind of Pseudomonas fluorescens Y13, and also relates to a preparation method of Pseudomonas fluorescens Y13, and also relates to a method of Pseudomonas fluorescens Y13 in dissolving soil organic phosphorus and increasing soil soluble phosphorus content. use. Background technique [0002] Phosphorus is one of the indispensable elements for plant growth. However, most of the phosphorus and Ca in the soil 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined with other cations, it exists in the form of insoluble metal chelates, resulting in the lack of available phosphorus in most soils. 74% of the cultivated land in my country is deficient in phosphorus, and more than 95% of the phosphorus in the soil cannot be directly absorbed and utilized by organisms. Even in the season when phosphorus fertilizer is applied, the phosphorus use efficiency of cro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/39
Inventor 陈雯莉黄巧云高怡
Owner HUAZHONG AGRI UNIV
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