Preservation method of tabasheer spores

A technology of spores and bamboo yellow, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve problems such as the difference in the level of strains of preservation effects, and achieve the effect of ensuring vitality

Active Publication Date: 2013-12-25
贵州省生物技术研究所
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AI Technical Summary

Problems solved by technology

[0011] The preservation effects of the above methods not only have differences in the strain level, but also are only suitable for the preservation of

Method used

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Examples

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Example Embodiment

[0021] The embodiment of the present invention: The method for preserving spores of bamboo yellow includes the following steps:

[0022] (1) Collection of bamboo yellow spores: in the middle stage of bamboo yellow growth, scrape the bamboo yellow spores into a clean kraft paper bag with tweezers soaked in medical alcohol with a concentration of 75% by mass to be processed;

[0023] (2) Spore pretreatment: Put the kraft paper bag containing bamboo yellow spores in the refrigerator at 4°C, store for 7 days and then take it out to obtain dry spore blocks;

[0024] (3) Vegetable oil pretreatment: Fill the vegetable oil with a clean glass bottle, put the glass bottle with vegetable oil in the steamer and steam for 1 hour, take it out and cool to room temperature for later use;

[0025] (4) Bamboo yellow spore storage: Put the dried spore block obtained in step (2) into the rape oil processed in step (3) for storage; the volume of rape oil is 5-10 times the volume of the spore block, and the...

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Abstract

The invention discloses a preservation method of tabasheer spores. Firstly, the tabasheer spores are scraped to be contained in a clean kraft paper bag to wait for being processed; the kraft paper bag in which the tabasheer spores are contained is placed into a refrigerator at 4 DEG C and taken out after being preserved for seven days, and dry spore blocks are obtained; vegetable oil is contained in a clean glass bottle, the glass bottle in which the vegetable oil is contained is placed into a steaming pot to be steamed for one hour, and then the glass bottle is taken out and cooled to be at room temperature for use; the obtained dry spore blocks are placed into the processed vegetable oil for preservation; at the stage of tabasheer inoculating in the next year, the vegetable oil for preserving the tabasheer spores is poured out, cool boiled water is added in for washing the spore blocks 1-2 times, and then the spore blocks are soaked with cool boiled water for two hours to obtain tabasheer spore liquid. The preservation method not only can make the tabasheer spores smoothly survive the severe environment in the wild and effectively ensure vigour of the tabasheer spores, but also can solve the problem that a conventional culture preservation method is not suitable for preservation of the tabasheer spores, therefore, provenance guarantees are provided for artificial cultivation of the tabasheer spores, and the key technical problem on preservation of inocula in promotion of the artificial cultivation of the tabasheer spores is solved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preserving bamboo yellow spores. Background technique [0002] bamboo yellow ( Shiraia bambusicola ) also known as Tianzhu flower, light bamboo flower, bamboo flower, red dumpling, bamboo red dumpling, bamboo cocoon, bamboo red spot fungus, light chrysanthemum, bamboo ginseng, bamboo notoginseng, blood notoginseng, etc., is a kind of bamboo that grows on bamboo branches Subunits of medicinal fungi. Its main component hypocreamin is the red pigment part of bamboo yellow, which is a mixture of various perylenequinones (PQ) derivatives. It is a new type of photosensitizer and is used in clinical treatment of skin diseases, stomach diseases and rheumatoid arthritis. Modern medical research shows that the medicinal value of hypocrellin is mainly reflected in anti-inflammatory, analgesic, antibacterial, anti-tumor, etc., especially in recent years, research has shown its ex...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 刘永翔刘作易周德群陈艺萌朱英黄永会
Owner 贵州省生物技术研究所
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