Multilevel sorting microfluidic device for rare cells

Abstract

The invention provides a multilevel sorting microfluidic device for rare cells. The multilevel sorting microfluidic device comprises a runner structure sealed through bonding and an electrode substrate, wherein the runner structure comprises a spiral runner, a second grade runner, a lower branch runner, an upper bifurcated runner and a lower bifurcated runner, one end of the spiral runner is a sample inlet, the other end of the spiral runner is connected with the second grade runner and the lower branch runner respectively through a shrink and expansion structure, the second grade runner is connected with the upper bifurcated runner and the lower bifurcated runner respectively, the upper bifurcated runner and the lower branch runner are connected with the outlet of a blood corpuscle, the lower bifurcated runner is connected with the outlet of the rare cell, the electrode substrate comprises a substrate body and two groups of electrode groups, and the electrode groups are arranged at the bottom of the second grade runner. The multilevel sorting microfluidic device for the rare cells has low cost, is simple to operate, is easily integrated into micromation, and can realize high flux and high purity sorting of rare cancer cells.

Description

technical field [0001] The invention belongs to the field of analysis and detection equipment, and relates to a microfluidic device for multi-stage sorting of rare cell particles, in particular to a high-flux, high-purity multi-stage sorting of rare cell particles that integrates microfluidic inertial effects and dielectrophoresis technology Microfluidic devices. Background technique [0002] Accurate detection of cells is of great significance in biomedical applications such as disease prevention, diagnosis, treatment, and cell biology research. But usually in clinical practice, the object of research is a non-homogeneous population, which contains a large amount of cell information, making it very difficult to detect rare cells; The ratio of the number of normal blood cells in the human body is about 1:10 9 , that is, there are only a few rare cancer cells in 1mL of blood, so detection is very difficult. [0003] As a new method of efficient fluid or particle manipulati...

AI Technical Summary

Problems solved by technology

Although the above methods have achieved certain research results, they still have their own shortcomings when applied to rare cell sorting: such as film sorting, squeeze flow sorting, hydraulic sorting, field flow sorting, gravity and sedimentation sorting. Separation, water-liquid two-phase separation, magnetic separation, and photoelectric separation all have the problem of low operating flux, which limits their further commercial applications; columnar array sorting, inertial flow separation, hydraulic separation, water-liquid separation Diphasic separation and other methods have the problem of low separation purity

However, the existing multi-stage sorting research is still based on a single technology. It is only a simple combination of a single sorting technology, which increases the operation process and cost, and it is difficult to satisfy the high-throughput and high-purity separation of rare cells at the same time. requirements

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