Method for degumming soybean blended oil with immobilized phospholipase A2

A technology of immobilizing phospholipase and mixed oil, which is applied in the direction of edible oil/fat, fat oil/fat refining, application, etc. It can solve the problems of reducing equipment utilization, clogging pipelines, forming, etc., and achieves cost reduction and good reusability , the effect of not easy to deform and deactivate

Inactive Publication Date: 2013-12-25
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems that the colloidal substances (mainly phospholipids) in the leached mixed oil are partially separated out, scales are formed in the equipment, pipes ar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0008] Specific embodiment one: use immobilized phospholipase A 2 The method for degumming soybean mixed oil is realized through the following steps: 1. Pre-evaporate soybean mixed oil to 40-65% of its original weight at 60-70°C and a vacuum of 0.06-0.08 MPa to obtain pre-evaporated Mixed oil; 2. Immobilized phospholipase A 2 For soybean mixed oil degumming: heat soybean mixed oil to 60°C, add about 0.1% of 45% citric acid solution for acid reaction and cool down to a certain temperature, add about 4% NaOH solution to adjust the initial pH to a certain value, Add an appropriate amount of hot soft water, and a certain amount of immobilized phospholipase A 2 Carry out the enzyme reaction for a certain period of time, and the degummed oil obtained is centrifuged in a disc centrifuge (explosion-proof) for 20 minutes, and then the upper oil sample is preserved, which is the enzymatic degummed mixed oil; 3. Immobilized phospholipase A 2 Recovery: the immobilized phospholipase A af...

specific Embodiment approach 2

[0009] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the degumming temperature in step 5 is 45° C. and then immobilized phospholipase A 2 Degumming of soybean blend oil. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0010] Specific embodiment three: the difference between this embodiment and specific embodiment one is that in step five, adjust the pH with about 4% NaOH solution to be 6.0 and then use immobilized phospholipase A 2 Degumming of soybean blend oil. Other steps are the same as in the first embodiment.

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Abstract

The invention provides a method for degumming soybean blended oil with immobilized phospholipase A2. The blended oil is preevaporated to increase the contact possibility between the oil and the immobilized enzyme, thereby reducing the inactivation of the immobilized enzyme. The method optimizes the optimum parameters of the reaction temperature, initial reaction pH, addition amount of the immobilized enzyme, reaction time and other degumming conditions. The immobilized phospholipase A2 is used for degumming the soybean blended oil, thereby enhancing the activity and stability of the enzyme in the reaction system, reducing the scaling and pipeline blockage caused by the precipitation of colloids (phospholipid and the like) in the oil. enhancing the utilization ratio of equipment, and lowering the oil refinement cost; besides, the phosphorus content in the final enzyme-method degummed soybean oil obtained by degumming the soybean blended oil with the immobilized phospholipase A2 can be obviously lowered to 11.56 mg/kg; the color of the obtained raw oil (Y35, R6.5, and Lovibond 25.4mm dispoable cuvettes) is better than the color of conventionally degummed oil (Y35, R7.6, and Lovibond 25.4mm dispoable cuvettes); and thus, the method has important meanings for oil refinement.

Description

technical field [0001] The invention relates to the field of oil processing technology, in particular to a method for using immobilized phospholipase A 2 Method for degumming mixed oils. Background technique [0002] Grease is a necessity in people's daily life. It plays a very important physiological function in the human body and is one of the energy sources of human life. The methods of oil production mainly include pressing oil and leaching oil, and leaching oil has become the oil production method favored by most oil companies because of its high oil yield and better quality of the obtained meal. The mixture of oil and solvent from the extractor is called mixed oil, and the mixed oil must undergo certain treatment to separate the solvent in the oil. [0003] Grease degumming aims to remove the phospholipids in the oil. The phospholipids in the oil are divided into hydratable phospholipids and non-hydratable phospholipids. The hydratable phospholipids are easier to rem...

Claims

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Application Information

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IPC IPC(8): C11B3/00C11B3/04C11B3/14A23D9/04
Inventor 江连洲王立琦潘明喆于殿宇张佳宁
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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