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Identification method of bean leaf webber resistance

The invention relates to a technology for the identification method of bean roll leaf borer, which can be applied in the directions of biological testing and material inspection, and can solve the problems of poor stability of identification results and achieve the effect of stable identification results.

Inactive Publication Date: 2013-12-25
广西壮族自治区农业科学院经济作物研究所
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Problems solved by technology

When using natural insect sources in the field to identify the resistance of bean leaf rollers, the occurrence of bean leaf rollers will vary greatly due to differences in years and environmental and climatic conditions. Some years are heavy, and some years are light. The result is less stable

Method used

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Experimental program
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Effect test

Embodiment

[0026] 1. Experimental conditions:

[0027] (1) Participating varieties: 22 varieties, see Table 1 for details;

[0028] (2) Experiment time and place: in the spring of 2010, the economic crop soybean experiment site of Guangxi Academy of Agricultural Sciences;

[0029] (3) Identification method:

[0030] a. Planting of insect-rearing soybean varieties: plant soybean materials accounting for one-third of the area of ​​the net room in the insect-proof net room for raising the bean leaf roller;

[0031] b. Planting of identified soybean varieties: Plant the soybean varieties to be identified and the high-resistance and high-sensitivity control soybean varieties in the same net room, with three repeated randomized block designs;

[0032] c. Collection and raising of bean leaf roller insect source: after the soybean grows to 10 leaves, collect the older larvae of the bean leaf roller in the field, and connect it to the soybean variety used for raising insects in the net room, an...

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Abstract

The invention relates to an identification method of bean leaf webber resistance. The identification method comprises the steps of planting a soybean to be identified in an insect-proof net room, feeding bean leaf webber larvae in the same net room, continuing feeding in the net room with food till pupation, emergence, mating, oviposition and incubation of the larvae to be injurious, investigating population density of the larvae, and identifying the insect resistance of a material according to a grading standard. The method can ensure adequate population density, and is not influenced by years or environmental conditions, so that an identification result is more stable, accurate and reliable.

Description

technical field [0001] The invention belongs to the field of insect resistance identification of crops, and in particular relates to an identification method for resistance to bean leaf rollers. [0002] Background technique [0003] Soybean is native to China and is an important grain and oil crop, occupying an important position in the world's agricultural production. On the one hand, soybean protein content is relatively high, which can reach more than 45%, and it is an important source of vegetable protein; on the other hand, soybean fat content is about 21%, which is an important source of edible oil. The international community attaches great importance to the production and development of soybeans. The trade volume of soybeans accounts for more than half of the total international trade of agricultural products, ranking first. [0004] The bean pyralid (Lamprosema indicata Fabricius) is an important soybean leaf-eating pest. It occurs in all soybean producin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 孙祖东杨守臻陈怀珠蔡昭艳唐向民
Owner 广西壮族自治区农业科学院经济作物研究所
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