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Tissue culture medium and in-vitro regeneration method for ficus pandurata blade

A technology of tissue culture medium and in vitro regeneration, applied in the field of plant tissue culture, can solve problems such as shedding, hindering promotion and application, affecting garden effects, etc., and achieve the effect of increasing differentiation rate

Active Publication Date: 2014-01-22
杭州瓦屋科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Ficus luteus has poor cold resistance, and when the temperature is lower than 5°C, the leaves will wither and turn yellow or even fall off, which affects its garden effect and hinders its promotion and application to northern regions.

Method used

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  • Tissue culture medium and in-vitro regeneration method for ficus pandurata blade
  • Tissue culture medium and in-vitro regeneration method for ficus pandurata blade
  • Tissue culture medium and in-vitro regeneration method for ficus pandurata blade

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Embodiment Construction

[0025] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0026] The test materials used in the embodiments of the present invention are taken from the greenhouse of the Flower Research Center of Chongqing University of Arts and Sciences. The culture conditions in the following experiments are all: after inoculation, at 25±2°C, culture in the dark for 2 weeks, and then culture under the condition of light intensity of 1500-2000 lx.

[0027] 1) Callus induction: Take the young leaves of the Ficus lyre plant grown in the greenhouse that are robust and free from diseases and insect pests, wash them with running water for 10-15 minutes, soak them in washing powder for 3-4 minutes, rinse them with clean water, take them out and put them in the ultra-clean work Surface disinfection with 75% ethanol for 30 seconds, 0.1% mercury liter disinfection for 3-5 minutes on the table, and finally rinse with steri...

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Abstract

The invention discloses a tissue culture medium and an in-vitro regeneration method for a ficus pandurata blade. According to an in-vitro regeneration system of the ficus pandurata blade established through the tissue culture medium and the in-vitro regeneration method, a callus induction medium comprises MS plus 2.0 mg / L of 6-BA plus 0.1mg / L of NAA, a callus proliferation medium comprises MS+6-BA 1.0mg / L+NAA 0.1mg / L, an adventitious bud differential medium MS+6-BA 1.0mg / L+NAA 0.1mg / L and a rooting medium 1 / 2MS+IBA 0.1mg / L+AC 300+carrageenan 6.0g / L+sucrose 30g / L; a blade with a petiole is arranged on an inoculated portion in the mode that the back of the blade makes contact with the tissue culture medium downwardly. By means of the tissue culture medium and the in-vitro regeneration method, in-vitro culture and adventitious bud regeneration of the ficus pandurata blade are subjected to optimized design, and therefore the inductivity of calluses and the differentiation rate of adventitious buds are increased.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a tissue culture medium of Ficus lyre leaves and an in vitro regeneration method. Background technique [0002] Ficus lyrata (Ficus lyrata) is an evergreen tree of the genus Ficus in the family Moraceae. The leaves are thick leathery, dark green, shiny on the surface, entire, with sunken veins, 15-38cm long and 10-17cm wide. It is named for its shape like a violin. Native to tropical Africa, it can be used as a garden tree, street tree, or potted tree; it likes a warm, humid, and sunny environment, with a suitable growth temperature of 25°C to 35°C, a dormant temperature of around 15°C, and a safe overwintering above 5°C; water requirements It is rather wet than dry; it has strong adaptability to light, and can grow well in bright scattered light, avoiding direct sunlight in summer; it is not strict with the soil, and prefers acidity, and is not resistant to barren and...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 娄娟陈泽雄
Owner 杭州瓦屋科技有限公司
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