Method for promoting Codonopsis pilosula seed germination by utilizing bacteriabeneficial beneficial bacteria

A technology of seeds and Codonopsis pilosula, applied in the fields of microbiology and plant physiology, can solve problems such as scattered resources, limited quantity of commodities, and contradiction between supply and demand in Codonopsis pilosula market, and achieve simple treatment methods, strong non-pathogenicity and stress resistance, and improved Effects on Vigor and Emergence

Inactive Publication Date: 2014-02-05
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the national Codonopsis ginseng wild resource reserve is about 15,800 tons, but the resources are scattered and the output is declining year by year. The number of commodities that can be provided is extremely limited, and the contradiction between supply and demand in the Codonopsis ginseng market is extremely prominent (Liu Dejun et al. 2001)

Method used

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  • Method for promoting Codonopsis pilosula seed germination by utilizing bacteriabeneficial beneficial bacteria
  • Method for promoting Codonopsis pilosula seed germination by utilizing bacteriabeneficial beneficial bacteria
  • Method for promoting Codonopsis pilosula seed germination by utilizing bacteriabeneficial beneficial bacteria

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Embodiment 1

[0016] Embodiment 1: a kind of method that rhizosphere beneficial bacteria promotes Codonopsis pilosula seed germination, its main feature is that the steps are:

[0017] (1) Disinfection treatment of Codonopsis pilosula seeds: Soak Codonopsis pilosula seeds with full grains, no defects, and uniformity in 75% alcohol for 8 seconds, then soak in 5% sodium hypochlorite for 10 minutes, rinse with sterilized distilled water for 7-8 times, and store in a refrigerator at 4°C overnight;

[0018] (2) Preparation of Bacillus subtilis GB03 bacterial liquid:

[0019] Prepare LB liquid medium first: prepare according to the ratio of sodium chloride 5g / L, tryptone 10g / L and yeast powder 5g / L, adjust the pH to 6.8 with a standard pH meter (PB-10, Beijing Sartorius), Use a vertical pressure steam sterilizer (LDZX-50KBS, Shanghai Shenan) to sterilize at a high temperature of 118-125 °C and wait for cooling. Add 50 μL of GB03 bacterial solution to every 100 mL of LB liquid medium, and at a sp...

Embodiment 2

[0021] Embodiment 2: a kind of method that rhizosphere beneficial bacteria promotes Codonopsis pilosula seed germination, its main feature is that the steps are:

[0022] (1) Disinfection treatment of Codonopsis pilosula seeds: Soak Codonopsis pilosula seeds with full grains, no defects, and uniformity in 75% alcohol for 9 seconds, then soak in 5% sodium hypochlorite for 11 minutes, rinse with sterilized distilled water for 7-8 times, and store in a refrigerator at 2°C overnight;

[0023] (2) Preparation of Bacillus subtilis GB03 bacterial liquid:

[0024] First prepare lysate broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, sterilize at 115-118°C at high temperature, and wait for cooling After the temperature is 20°C, add 80 μL of Bacillus subtilis GB03 bacterial liquid to every 100 mL of LB liquid medium, and measure the concentration of the bacterial liquid a...

Embodiment 3

[0026] Embodiment 3: a kind of method that rhizosphere beneficial bacteria promotes Codonopsis pilosula seed germination, its main feature is that the steps are:

[0027] (1) Disinfection treatment of Codonopsis pilosula seeds: Soak Codonopsis pilosula seeds with full grains, no defects, and uniformity in 75% alcohol for 10 seconds, then soak in 5% sodium hypochlorite for 12 minutes, rinse with sterilized distilled water for 7-8 times, and store in a refrigerator at 4°C overnight;

[0028] (2) Preparation of Bacillus subtilis GB03 bacterial liquid:

[0029] First prepare lysate broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, sterilize at 125-132°C at high temperature, and wait for cooling After the temperature is 35°C, add 100 μL of Bacillus subtilis GB03 bacterial liquid to every 100 mL of LB liquid medium, and measure the concentration of the bacterial liquid...

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Abstract

The invention relates to a method for promoting Codonopsis pilosula seed germination by utilizing bacteriabeneficial beneficial bacteria. The method utilizes solution of Bacillus subtilis belonging to bacteriabeneficial growth promotion microorganisms to steep Codonopsis pilosula seeds, therefore, germination potential and germination rate of the Codonopsis pilosula seeds are remarkably increased, vigor and emergence rate of the Codonopsis pilosula seeds are increased, problems of small Codonopsis pilosula seeds, low germination potential and germination rate and irregular emergence during production are solved, and economic losses on labor and material resources caused by seedling supplement and repeated germination of the Codonopsis pilosula are reduced. Furthermore, the Bacillus subtilis has the advantages of being free of toxicity and harm to people and livestock, free of pollution to environment, non-pathogenic and strong in stress resistance, and can produce a variety of growth hormone-like chemicals and rich antimicrobial substances, promote plant growth and improve plant growth and development. The method is simple, can be widely applied to cultivation of the Codonopsis pilosula, and has important social, economic and ecological economic value.

Description

technical field [0001] The invention belongs to the fields of microbiology and plant physiology, and relates to a method for improving the germination potential and germination rate of Codonopsis pilosula seeds by using rhizosphere beneficial bacteria Bacillus subtilis GB03 strain, thereby promoting the germination of Codonopsis pilosula seeds, and ensuring robust and uniform emergence in the field after seeds are sown. Background technique [0002] Bacillus subtilis has the characteristics of non-toxic and harmless to humans and animals, no pollution to the environment, non-pathogenicity and strong stress resistance. It can produce a variety of growth hormone substances and rich antibacterial substances to promote plant growth. Improve plant growth and development, increase plant resistance to salt stress and other adversities, thereby improving plant disease resistance. [0003] In recent years, Codonopsis pilosula has made new progress in the treatment of coronary heart d...

Claims

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Application Information

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IPC IPC(8): A01G7/06
Inventor 张金林吴永娜王锁民吕昕培李静韩庆庆包爱科
Owner LANZHOU UNIVERSITY
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