Burnet eucalyptus extract for treating AIDS and preparation method thereof

Abstract

The invention provide a sanguisorba extract for treatment of AIDS (acquired immunodeficiency syndrome) and a preparation method thereof, and the sanguisorba extract mainly comprises gallic acid, ellagic acid, hydrolysable tannin and condensed tannin. The sanguisorba extract has excellent inhibitory activity and low cytotoxicity to R5-tropic and X4-tropic AIDS viruses.

Description

technical field

[0001] The invention relates to a Burnet eucalyptus extract and a preparation method thereof, in particular to a Burnet eucalyptus extract for treating AIDS and a preparation method thereof. Background technique

[0002] Burnet is the dry root of Sanguisorba officinalis L. or Sanguisorba officinalis L.var.longifolia (Bert.) YüetLi, a plant of the Rosaceae family. The effect of stopping bleeding, detoxifying and suppressing sores.

[0003] The research on the chemical constituents of Burnet mainly focuses on tannins, triterpenes and triterpene saponins, flavonoids and steroids, among which tannins, triterpenes and triterpene saponins have the highest content. Tannin is considered to be the material basis for its astringent and hemostatic effect.

[0004] Tannins, also known as tannins, are a class of polyphenolic compounds with complex structures existing in plants. In 1920, KFrendenberg divided tannins into two categories according to their chemical struct...

Examples

Embodiment 1

[0040] Take 100 g of coarse powder of Sangui medicinal material, decoct and extract twice with 10 times of water (V / W), 1 hour each time, combine the two extracts, concentrate to dryness under reduced pressure, and obtain 13.7 g of Sangui water extract.

Embodiment 2

[0042] Burnet eucalyptus powder 1000g, ultrasonic extraction with 10 times the amount (V / W) of 60% ethanol twice, each time for 1 hour, the two extracts were combined, concentrated under reduced pressure until alcohol-free, and then washed with ethyl acetate Esters and n-butanol were extracted 3 times respectively to obtain 3 extracts with different polarities: ethyl acetate extract-I (ETOAC-I) 10.2g, n-butanol extract-I (BUOH-I) 18.4g and Aqueous residue (DW) 59.3g.

Embodiment 3

[0044] Burnet elm crude powder 100g, reflux extraction with 10 times the amount (V / W) of 60% ethanol for 2 times, each time for 1 hour, combine the two extracts, concentrate under reduced pressure until no alcohol smell, and obtain 60% alcohol extract 12.5g; the ethanol extract was extracted 5 times with ethyl acetate, the combined extracts were recovered to dryness under reduced pressure, and the ethyl acetate extract-II (ETOAC-II) of Burnet was obtained.

technical field

[0001] The invention relates to a Burnet eucalyptus extract and a preparation method thereof, in particular to a Burnet eucalyptus extract for treating AIDS and a preparation method thereof. Background technique

[0002] Burnet is the dry root of Sanguisorba officinalis L. or Sanguisorba officinalis L.var.longifolia (Bert.) YüetLi, a plant of the Rosaceae family. The effect of stopping bleeding, detoxifying and suppressing sores.

[0003] The research on the chemical constituents of Burnet mainly focuses on tannins, triterpenes and triterpene saponins, flavonoids and steroids, among which tannins, triterpenes and triterpene saponins have the highest content. Tannin is considered to be the material basis for its astringent and hemostatic effect.

[0004] Tannins, also known as tannins, are a class of polyphenolic compounds with complex structures existing in plants. In 1920, KFrendenberg divided tannins into two categories according to their chemical struct...

Examples

Embodiment 1

[0040] Take 100 g of coarse powder of Sangui medicinal material, decoct and extract twice with 10 times of water (V / W), 1 hour each time, combine the two extracts, concentrate to dryness under reduced pressure, and obtain 13.7 g of Sangui water extract.

Embodiment 2

[0042] Burnet eucalyptus powder 1000g, ultrasonic extraction with 10 times the amount (V / W) of 60% ethanol twice, each time for 1 hour, the two extracts were combined, concentrated under reduced pressure until alcohol-free, and then washed with ethyl acetate Esters and n-butanol were extracted 3 times respectively to obtain 3 extracts with different polarities: ethyl acetate extract-I (ETOAC-I) 10.2g, n-butanol extract-I (BUOH-I) 18.4g and Aqueous residue (DW) 59.3g.

Embodiment 3

[0044] Burnet elm crude powder 100g, reflux extraction with 10 times the amount (V / W) of 60% ethanol for 2 times, each time for 1 hour, combine the two extracts, concentrate under reduced pressure until no alcohol smell, and obtain 60% alcohol extract 12.5g; the ethanol extract was extracted 5 times with ethyl acetate, the combined extracts were recovered to dryness under reduced pressure, and the ethyl acetate extract-II (ETOAC-II) of Burnet was obtained.

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