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Plant pollen development specific promoter and application thereof

A plant pollen and promoter technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve the problem that there is no promoter specifically expressed in the development of cloned pollen, and achieve good application prospects

Inactive Publication Date: 2014-02-26
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The promoter specifically expressed by this pollen development has not been cloned yet

Method used

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  • Plant pollen development specific promoter and application thereof
  • Plant pollen development specific promoter and application thereof
  • Plant pollen development specific promoter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0017] The plant pollen development-specific promoter of the present invention, its preferred embodiment is:

[0018] Including the following nucleotide sequences:

[0019] ·AATTCATTACATATAACAGCGCCGTAATAACTTGGATCTCATCTATACGTGTGTGAGTTGTAGATATATAA ACAAAATTTATTTTCATTTGTGAAAAAAATATTTATACTAGATTTTTAGATGTGGAAATAGTGCAACAAGC AAAAAGTTACAAATAAAAGAAAATGTGGAAATATGAGGTATAAAATAAAATAGAGAACTAGGACTAGGAGA CTTGTTACTTTGTTAGTGCATGTAGTGGTAAATAACATAAAGTCCATTCAGAACTAGACTATTCTCACCTC TCCAACAATGAACTTAATATAGGCTGCTGGCCTGTTGTGGCCCAGAAAAAGTTAAAGAAGAACAACAAAAA CATGAATTCATTTCCGGTATAAGGTGAAAGATACACATGAAAAATGGTGAGTAGCGTTCAGAAAAGGAAAA GTAACTCAATAGTACAAAAGAAAATCTATTTCCACAATACAAAATAAAACTCATTAATCATGTAGTATCCT TTTTCCTAAATTTCATATTGGATTAATCTTTTAATTTTTTGGTTTGATTGGTAGATCTATGTAATGCATGT ATACCACTTTAACACAAACCTTTTTTCTTACAATTTTTCTTTATGTATTTATTTTTGGTTGGTTTTTATCC CAAAACAGACTAATACAATGCGTGTGTGATGCTTTCACTCTTTCTCAAGCACAATCCGCCTAGACTATGGT CACGTATTCACTCATTTCTTCTTTACTTATTAAAATGTAAAAAATCTGATTAATAATTTTCATTTAATTCT TGCTTTGTCTTTTCATCACCAAACTCA...

specific Embodiment

[0024] Concrete embodiment, as shown in the table below:

[0025]

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Abstract

The invention discloses a plant pollen development specific promoter and an application thereof. The promoter has a full length of 1140 bases, and is used for starting gene expression at the plant pollen mononuclear microspore phase and at the matured pollen grain phase, wherein the expression is only carried out in tapetal layers and pollen. The promoter has good application prospects on genetic engineering breeding, is helpful for understanding biological phenomena of pollen development and male sterility, provides a new regulatory element for plant male sterility genetic engineering, and provides theoretical basis and application guidance for effective control of plant fertility performance, and rapid acquisition of production-available male sterile lines.

Description

technical field [0001] The present invention relates to a transcriptional gene promoter, in particular to a plant pollen development specific promoter and application thereof. Background technique [0002] Plant male sterility refers to the phenomenon that the abnormal anthers of hermaphrodite plants lead to the loss of pollen function, which is common in angiosperms. Breeding male sterile lines using the male sterility characteristics of plants is an important means to simplify the seed production procedure and reduce the cost of seed production in the utilization of crop heterosis. Brassicaceae crops are the most common type of heterosis utilization, male sterile line is an ideal system for producing a generation of hybrids. From the perspective of developmental molecular biology, pollen abortion is the phenotypic manifestation of male sterility in plants. Understanding the whole process and molecular mechanism of pollen development is the basis and key to the study of ma...

Claims

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Application Information

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IPC IPC(8): C12N15/113
Inventor 韩莹琰范双喜程继鸿宋婷婷田佶杨凯
Owner BEIJING UNIV OF AGRI