Radix paeoniae rubrathe liver-protecting active component, and preparation method and purpose thereof

A technology of red peony root and effective components is applied in the application field of preparing liver-protecting drugs, can solve the problems of unclear liver-protecting effect and the like, and achieve the effects of good pharmacological activity and simple separation process

Inactive Publication Date: 2014-03-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far only paeoniflorin in Radix Paeoniae Rubra has been found to protect the liver, but it is not clear whether other active ingredients in Radix Radix Radix Paeoniae Rubra have hepatoprotective effects

Method used

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  • Radix paeoniae rubrathe liver-protecting active component, and preparation method and purpose thereof
  • Radix paeoniae rubrathe liver-protecting active component, and preparation method and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Preparation of effective components of Radix Paeoniae Rubra

[0015] After crushing 200 g of Radix Paeoniae Rubra, add 1 L of ethyl acetate, heat and reflux for extraction for 2 hours to obtain an extract, and concentrate the extract to dryness under reduced pressure to obtain 3.2 g of extract. Mix the extract with 50g of forward silica gel, separate it with a normal phase silica gel column (column height 15cm, diameter 4.65cm), and use petroleum ether (60-90°C) and chloroform with a volume ratio of 3:1 As mobile phase elution, the eluate of the first 3 column volumes was discarded, and the eluate of the fourth column volume was collected, concentrated under reduced pressure, and vacuum-dried to obtain 0.5 g of the effective component.

Embodiment 2

[0016] Example 2 Preparation of effective components of Radix Paeoniae Rubra

[0017] After crushing 500g of Radix Paeoniae Rubra, add 3L of absolute ethanol, heat and reflux for extraction for 2 hours to obtain an extract, and concentrate the extract to dryness under reduced pressure to obtain 70.2g of extract. Mix the extract with 250 g of forward silica gel, separate it with a normal phase silica gel column (column height 50 cm, diameter 7 cm), and use a volume ratio of 1:1 cyclohexane and ethyl acetate as the mobile phase for elution , remove the eluate of the first 3 column volumes, collect the eluate of the 4th column volume, concentrate it under reduced pressure, and vacuum dry to obtain 1.3 g of effective components.

Embodiment 3

[0018] The preparation of embodiment three Radix Paeoniae Rubra effective components

[0019] Take 250 g of Radix Paeoniae Rubra, crush it, add 1.5 L of ethyl acetate and ethanol solution with a volume ratio of 1:1, heat and reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract was concentrated to dryness under reduced pressure to obtain 23.2 g of extract. Mix the extract and 100 g of silica gel, separate it with a normal-phase silica gel column (column height 25 cm, diameter 4.65 cm), use petroleum ether and ethyl acetate (volume ratio: 50:1) as mobile phase elution, discard The eluate of 3 column volumes was collected, and the eluate of the 4th column volume was collected, concentrated under reduced pressure, and vacuum-dried to obtain 0.7 g of the effective component.

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PUM

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Abstract

The invention provides a radix paeoniae rubrathe active component, mainly containing 40%-52% of a compound A ethyl palmitate and 30%-48% of a compound B ethyl linoleate. The medicinal material radix paeoniae rubrathe is crushed, added with ethyl acetate and ethanol and subjected to heating extraction to obtain an extracting solution; the extracting solution is concentrated into an extract; the extract is mixed with silica gel; the mixture is separated by a normal phase silica gel column with petroleum ether and ethyl acetate as mobile phases, so as to obtain an eluent; and the eluent is subjected to concentration and drying to obtain the active component. The extraction separation process provided by the invention is simple and can quickly and accurately obtain the active component. Pharmacological experiments confirm that the active component has hepatoprotective effect and can be used in preparation of medicaments for the treatment of non-alcohol liver injury.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a preparation method of an effective component containing ethyl palmitate and ethyl linoleate from Radix Paeoniae Rubra, and an application of the effective component in preparing liver-protecting medicines. Background technique [0002] Liver injury is a complex process caused by multiple etiologies and involving multiple factors. Liver disease caused by liver injury has become a common and frequently-occurring disease. What is more serious is the liver failure and hepatic encephalopathy caused by it. The morbidity and mortality are high, which greatly threatens human health. Many factors can cause liver damage. In vivo models, especially chemical liver injury models, are similar to human liver diseases in some aspects of morphology and pathophysiology. HepG2 cells have relatively complete liver drug metabolism phase I and phase II enzymes, which have be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/231A61K36/71A61P1/16A61K31/22
Inventor 程翼宇赵筱萍王毅张玉峰
Owner ZHEJIANG UNIV
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